Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Les auteurs tiennent à remercier la Division de Vector-Borne Direction générale des ressources animales maladies, en particulier Andrea Peterson, Lisa Massoudi, Verna O&#39;Brien, et John Liddell pour leur entretien et de maintenance des souris et des lapins. Nous aimerions également remercier Amy Ullmann, Theresa Russell, et Barbara J. Johnson pour leurs contributions à l&#39;égard de ce manuscrit. Enfin, nous tenons à remercier Alissa Eckert dans le bureau du directeur adjoint pour la communication à la CDC pour la réalisation des illustrations graphiques et Judy Lavelle pour diriger tous les aspects juridiques liés à la prise de vue de ce manuscrit.

1. Collecte hémolymphe de préparation des lames (Film 1) <ol><li> Retirez délicatement activement tiques d&#39;alimentation d&#39;un animal et le lieu dans le peroxyde d&#39;hydrogène à 3% topique pour 5 minutes, puis dans l&#39;éthanol à 70% pendant 10 minutes à la surface de stériliser. </li><li> Avec un stylo pap dessiner un cercle sur une lame de microscope enduite de silane et de placer la tique dans le cercle stylo pap. <ol><li> Silane lames enduites sont utilisés pour une ...

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M., Fikrig, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+and+migration+of+Borrelia+burgdorferi+in+Ixodes+ticks+during+blood+feeding.">Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding.</a> Am. J. Trop. Med. Hyg. 53, 397-404 (1995).</li><li>Horka, H., Cerna-Kyckova, K., Skallova, A., Kopecky, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tick+saliva+affects+both+proliferation+and+distribution+of+Borrelia+burgdorferi+spirochetes+in+mouse+organs+and+increases+transmission+of+spirochetes+to+ticks.">Tick saliva affects both proliferation and distribution of Borrelia burgdorferi spirochetes in mouse organs and increases transmission of spirochetes to ticks.</a> Int. J. Med. Microbiol. 299, 373-380 (2009).</li><li>Brossard, M., Wikel, S. 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La collection de l &#39;hémolymphe tique, les glandes salivaires et la salive est important dans l&#39;étude des tiques transmission d&#39;agents pathogènes, la prévalence, la diffusion, la prolifération, et la persistance dans les deux la tique et l&#39;hôte 6,11-13,20,23,29. Il ya plusieurs façons de disséquer une tique 30,31. Toutefois, lors de la collecte des glandes salivaires, il est essentiel de disséquer la tique proprement les glandes salivaires ne sont pas rompues ou perdu dans l...

<table border="1"><tbody><tr><td> Réactif </td><td> Entreprise </td><td> Numéro de catalogue </td></tr><tr><td> Le peroxyde d&#39;hydrogène </td><td> Pêcheur </td><td> H312-500 </td></tr><tr><td> L&#39;éthanol </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> Bioproduits Boston </td><td> BM-2205 </td></tr><tr><td> Dumont fine pince (3C) </td><td> Pêcheur </td><td> NC9906085 </td></tr><tr><td> Silane lames de microscope traitées </td><td> Bioworld </td><td> 42763007-1 </td></tr><tr><td> Stylo Pap </td><td> Bioworld </td><td> 21750008-1 </td></tr><tr><td> Super Frost, plus des lames de microscope </td><td> Pêcheur </td><td> 12-550-18 </td></tr><tr><td> Pilocarpine </td><td> Sigma </td><td> P6503-5G </td></tr><tr><td> Cocktail d&#39;inhibiteurs de protéase </td><td> Sigma </td><td> P2714 </td></tr><tr><td> # 11 scalpel jetable </td><td> Plume </td><td> 2975 # 11 </td></tr><tr><td> Pâte à modeler non toxique </td><td&gt; Fisher </td><td> S17307 </td></tr><tr><td> Les tubes capillaires </td><td> Chase scientifiques verre, inc </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Les tiques se trouvent dans le monde entier et affligent les humains avec de nombreuses maladies transmises par les tiques. Les tiques sont des vecteurs d&#39;agents pathogènes qui causent la maladie de Lyme et fièvre récurrente à poux (Borrelia spp.), Rocky fièvre pourprée des montagnes (Rickettsia rickettsii), l&#39;ehrlichiose (Ehrlichia chaffeensis et E. equi), l&#39;anaplasmose (Anaplasma phagocytophilum), l&#39;encéphalite (tic- virus de l&#39;encéphalite transmise par), de la babésiose (Babesia spp.), de la fièvre à tiques du Colorado (Coltivirus), et la tularémie (Francisella tularensis) 1-8. Pour être correctement transmis à l&#39;hôte de ces agents infectieux différentielle réguler l&#39;expression génique, interagir avec des protéines de tiques, et migrent à travers la tique 3,9-13. Par exemple, l&#39;agent de la maladie de Lyme, Borrelia burgdorferi, s&#39;adapte à travers l&#39;expression différentielle des gènes aux stades de fête et de la famine de cycle enzootique de la tique 14,15. En outre, comme une tique Ixodes consomme unerepas de sang Borrelia reproduire et migrer de l&#39;intestin moyen dans le hémocèle, où ils se rendent aux glandes salivaires et sont transmis à l&#39;hôte avec la salive expulsées 9,16-19. Comme une tique se nourrit de l&#39;hôte répond généralement avec une forte réponse immunitaire innée et hémostatique 11,13,20-22. En dépit de ces réponses de l&#39;hôte, I. scapularis peut nourrir pendant plusieurs jours en raison salive de la tique contient des protéines qui sont immunomodulateur, des agents lytiques, les anticoagulants, et fibrinolysins pour aider la tique nourrir 3,11,20,21,23. Les activités immunomodulatrices possédés par la salive de tique ou extrait des glandes salivaires (SGE) de faciliter la transmission, la prolifération et la diffusion de nombreux agents pathogènes transmis par les tiques 3,20,24-27. Pour mieux comprendre comment tiques agents infectieux provoquer une maladie, il est essentiel de disséquer les tiques se nourrissent activement et de recueillir la salive des tiques. Ce protocole vidéo montre les techniques de dissection pourla collection de l &#39;hémolymphe et la suppression des glandes salivaires de nourrir activement I. nymphes scapularis après 48 et 72 heures le placement de la souris après. Nous démontrons également le prélèvement de salive d&#39;un adulte femelle I. tique scapularis.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

La collection de l &#39;hémolymphe tique infectée, des glandes salivaires et la salive est important d&#39;étudier comment les tiques agents pathogènes causent la maladie. Dans ce protocole, nous montrer comment recueillir les glandes salivaires et l&#39;hémolymphe de se nourrir<em&gt; Ixodes scapularis</em&gt; Nymphes. Nous démontrons également le prélèvement de salive de la femelle<em&gt; I. scapularis</em&gt; Les adultes.

La salive, les glandes salivaires, et Collection hémolymphe de tiques Ixodes scapularis

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

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Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

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Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

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Préparation du murin des glandes salivaires sous-maxillaires pour la microscopie intravitale verticale

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Purification des préparations extracellulaires de vésicule à haut rendement loin du virus

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Divulgation de la collection d’hémolymphe et inoculation des blastospores de Métarhizium dans les tiques de Rhipicephalus MICROPLUS vers les études de pathologie des invertébrés

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...