Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Gli autori desiderano ringraziare la Divisione di Vector-Borne Diseases Branch Animal Resources, in particolare Andrea Peterson, Lisa Massoudi, Verna O&#39;Brien e John Liddell per la loro cura e manutenzione dei topi e conigli. Vorremmo anche ringraziare Amy Ullmann, Theresa Russell, e Barbara J. Johnson per il loro contributo verso questo manoscritto. Infine, vorremmo riconoscere Alissa Eckert presso l&#39;Ufficio del Direttore Associato per la Comunicazione presso il CDC per la produzione di illustrazioni grafiche e Judy Lavelle per dirigere tutti gli aspetti legali connessi con le riprese di questo manoscritto.

1. Raccolta emolinfa per preparazione del vetrino (Movie 1) <ol><li> Delicatamente rimuovere attivamente zecche alimentazione da un animale e posto in 3% di perossido di idrogeno topico per 5 minuti e poi in etanolo 70% per 10 minuti per sterilizzare superficie. </li><li> Con una penna pap disegnare un cerchio su un vetrino da microscopio silano rivestito e mettere il segno di spunta all&#39;interno del cerchio penna pap. <ol><li> Silano vetrini rivestiti vengono utilizzati per la migliore...

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La raccolta di emolinfa tick, ghiandole salivari, e la saliva è importante nello studio delle zecche trasmissione dei patogeni, la prevalenza, la diffusione, la proliferazione, e la persistenza sia il segno di spunta e l&#39;host 6,11-13,20,23,29. Ci sono diversi modi per sezionare un segno di spunta 30,31. Tuttavia, quando la raccolta delle ghiandole salivari è fondamentale per analizzare correttamente il segno di spunta in modo che le ghiandole salivari non sono rotti o persi nei resti della ze...

<table border="1"><tbody><tr><td> Reagente </td><td> Azienda </td><td> Numero di catalogo </td></tr><tr><td> Il perossido di idrogeno </td><td> Pescatore </td><td> H312-500 </td></tr><tr><td> Etanolo </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> Boston Bioproducts </td><td> BM-2205 </td></tr><tr><td> Dumont fine pinza (3C) </td><td> Pescatore </td><td> NC9906085 </td></tr><tr><td> Silano vetrini da microscopio trattati </td><td> Bioworld </td><td> 42763007-1 </td></tr><tr><td> Pap penna </td><td> Bioworld </td><td> 21750008-1 </td></tr><tr><td> Super gelo più vetrini da microscopio </td><td> Pescatore </td><td> 12-550-18 </td></tr><tr><td> Pilocarpine </td><td> Sigma </td><td> P6503-5G </td></tr><tr><td> Cocktail di inibitori della proteasi </td><td> Sigma </td><td> P2714 </td></tr><tr><td> # 11 monouso bisturi </td><td> Piuma </td><td> 2975 # 11 </td></tr><tr><td> Modellare l&#39;argilla non tossico </td><td&gt; Fisher </td><td> S17307 </td></tr><tr><td> Tubi capillari </td><td> Chase scientifica Glass, inc </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Le zecche sono presenti in tutto il mondo e di affliggere gli esseri umani con molte malattie trasmesse da zecche. Le zecche sono vettori di agenti patogeni che causano la malattia di Lyme e zecche febbre ricorrente (Borrelia spp.), Febbre delle Montagne Rocciose (Rickettsia rickettsii), ehrlichiosi (Ehrlichia chaffeensis e E. equi), anaplasmosi (Anaplasma phagocytophilum), encefalite (tick- virus dell&#39;encefalite Borne), babesiosi (Babesia spp.), febbre tick Colorado (Coltivirus), e la tularemia (Francisella tularensis) 1-8. Per essere correttamente trasmessi nell&#39;ospite questi agenti infettivi differenziale regolare l&#39;espressione genica, interagiscono con le proteine ​​zecca, e migrano attraverso il segno di spunta 3,9-13. Ad esempio, l&#39;agente della malattia di Lyme, Borrelia burgdorferi, si adatta attraverso l&#39;espressione genica differenziale alle fasi festa e la carestia del ciclo di leucosi della zecca 14,15. Inoltre, come una zecca Ixodes consumafarina di sangue Borrelia replicarsi e migrare dal midgut nel hemocoel, dove si spostano per le ghiandole salivari e vengono trasmessi nell&#39;ospite con la saliva espulsi 9,16-19. Come un segno di spunta alimenta l&#39;host risponde in genere con una forte risposta immunitaria innata emostatico e 11,13,20-22. Nonostante queste risposte di accoglienza, I. scapularis può alimentare per diversi giorni, perché la saliva tick contiene proteine ​​che sono immunomodulatori, agenti litici, anticoagulanti e fibrinolysins per aiutare il segno di spunta di alimentazione 3,11,20,21,23. Le attività immunomodulanti possedute dal saliva tick o estratti di ghiandola salivare (SGE) facilitare la trasmissione, la proliferazione e la diffusione di numerose zecche patogeni 3,20,24-27. Per capire meglio come zecche agenti infettivi causare la malattia è indispensabile analizzare attivamente l&#39;alimentazione zecche e raccogliere la saliva tick. Questo protocollo video mostra tecniche di dissezione perla raccolta di emolinfa e la rimozione delle ghiandole salivari da attivamente alimentazione I. ninfe scapularis dopo 48 e 72 ore dopo il posizionamento del mouse. Dimostriamo anche il prelievo della saliva da una femmina adulta I. scapularis tick.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

La raccolta di emolinfa zecca infetta, ghiandole salivari, e la saliva è importante studiare come zecche patogeni causare la malattia. In questo protocollo si dimostra come raccogliere emolinfa ghiandole salivari e di nutrirsi<em&gt; Ixodes scapularis</em&gt; Ninfe. Dimostriamo anche il prelievo della saliva da femmina<em&gt; I. scapularis</em&gt; Adulti.

Saliva, le ghiandole salivari, e Collection emolinfa dalle zecche Ixodes scapularis

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

RNA interferenza nelle zecche

Ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors ...

Ricerca

Immunologia e infezioni

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Ibridazione<em&gt; In situ</em&gt; Delle ghiandole salivari, ovaie, e gli embrioni di Vector zanzare

Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes.  Investigation of transcripts and gene ...

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Alimentazione di zecche su animali per trasmissione e Xenodiagnosis a Lyme Disease Research

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Lo sviluppo di un anticorpo salivare Multiplex immunoenzimatico per misurare l&#39;esposizione umana ad agenti patogeni ambientali

The etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

impennata<em&gt; Ixodes scapularis,</em&gt; Il Tick Black-legged: Alimentazione stadi immaturi sui topi

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

A Method for the Measurement of Salivary Gland Function in Mice

Un metodo per la misurazione della funzione della ghiandola salivaria in topi

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Preparazione della ghiandola salivare sottomandibolare murino per microscopia Intravital verticale

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Isolamento delle cellule epiteliali salivari dalle ghiandole salivari umane per la crescita in vitro come Salispheres o Monostrati

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purificazione dei preparati vescicoli extracellulari ad alto rendimento lontano dal virus

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Rivelare la raccolta di hemolymph e l'inoculo di Metarhizium blastospores in Rhipicephalus MicroPlus zecche verso gli studi di patologia degli invertebrati

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...