Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

著者らは、マウスやウサギの彼らの手入れや、保守のために特別にベクトルを媒介とする疾病の動物資源支店の部門、アンドレアソン、リサマスウーディー、ヴァーナ·オブライエン、ジョン·リデルに感謝します。また、この原稿に向かって彼らの貢献のためにエイミー·ウルマン、テレサ·ラッセル、バーバラJ.ジョンソンに感謝します。最後に、この原稿の撮影に関連付けられているすべての合法性を導くためのグラフィックイラストやジュディ·ラヴェルを製造するためのCDCのコミュニケーションのためのアソシエートディレクターのオフィスでアリッサ·エッカートを確認したいと思います。

1。スライドの準備のために体液のコレクション （動画1） <ol><li>穏やかに表面殺菌に10分間、5分間、その後70％エタノールで3％、局所過酸化水素の動物や場所からダニを供給積極的に削除します。 </li><li> PAPペンでシランコーティングされた顕微鏡スライド上に円を描くとPAPペンサークル内での目盛りを配置します。 <ol><li>シランコートしたスライドは、顕微鏡スライ?...

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ダニの体液、唾液腺、唾液のコレクションはダニ媒介性病原体の伝播の研究に重要であり、有病率、普及、増殖、目盛り、およびホスト6,11-13,20,23,29両方の持続性。目盛り30,31を分析する方法はいくつかあります。しかし、唾液腺を収集するときに唾液腺が破裂したり、ダニの遺骨が失われないように適切に目盛りを細かくすることが重要です。唾液腺は、それらが腸の汚染を...

<table border="1"><tbody><tr><td> 試薬 </td><td> 会社 </td><td> カタログ番号 </td></tr><tr><td>過酸化水素</td><td>フィッシャー</td><td> H312-500 </td></tr><tr><td>エタノール</td><td>アクロス</td><td> 61509-5000 </td></tr><tr><td> PBS </td><td>ボストンのバイオ製品</td><td> BM-2205 </td></tr><tr><td>デュモンファイン鉗子（3C） </td><td>フィッシャー</td><td> NC9906085 </td></tr><tr><td>シラン処理顕微鏡スライド</td><td> Bioworld </td><td> 42763007から1 </td></tr><tr><td> PAPペン</td><td> Bioworld </td><td> 21750008から1 </td></tr><tr><td>スーパーフロストプラス顕微鏡スライド</td><td>フィッシャー</td><td> 12-550-18 </td></tr><tr><td>ピロカルピン</td><td>シグマ</td><td> P6503-5G </td></tr><tr><td>プロテアーゼ阻害剤カクテル</td><td>シグマ</td><td> P2714 </td></tr><tr><td> ＃11使い捨てメス</td><td>フェザー</td><td> 2975＃11 </td></tr><tr><td>無毒粘土</td><td&gt;フィッシャー</td><td> S17307 </td></tr><tr><td>キャピラリーチューブ</td><td>チェイス科学グラス株式会社</td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

ダニは世界中に存在し、多くのダニ媒介性疾患を持つ人間を苦しめるされています。ダニはライム病とダニ媒介性回帰熱（ ボレリア属）、ロッキー山紅斑熱（ ロッキー山紅斑熱リケッチア ）、エーリキア症（ エールリヒアchaffeensisとE.エクイ ）、アナプラズマ病（ アナプラズマphagocytophilum）、脳炎（ダニの原因となる病原体のベクトルです。媒介性脳炎ウイルス）、バベシア症（ バベシア属）、コロラドダニ熱（Coltivirus）と、野兎病（ 野兎病菌 ）1-8。適切にホストに送信するこれらの感染性病原体は、差動、遺伝子発現を調節する目盛りのタンパク質と相互作用し、ダニ3,9-13を通して移行します。たとえば、ライム病剤、 ボレリアburgdorferiはは 、ダニの風土病サイクル14,15の祝宴と飢饉段階に差動遺伝子発現を介して適応します。また、 マダニティックとしては、消費吸血ボレリアは、唾液腺に移動すると追放された唾液9,16-19を使用してホストに送信され血体腔、腸内から複製と移行します。 ダニが供給されたホストは、通常、強力な止血及び先天性免疫応答11,13,20-22で応答します。これらのホストの応答にもかかわらず、I.ダニの唾液が3,11,20,21,23を供給するダニを支援するために免疫であるタンパク質、溶解剤、抗凝固剤、及びfibrinolysinsが含まれているため、scapularisは数日間供給できます。ダニの唾液や唾液腺抽出物（SGE）が保有する免疫調節活性は、多数のダニ媒介性病原体3,20,24-27の送信、増殖、普及を促進します。それは積極的に摂食ダニを分析し、ダニの唾液を収集することが不可欠である、さらにダニ媒介性感染症の病気を引き起こす方法を理解する。このビデオプロトコルは、解剖の技術を示しています体液の収集と積極的にIを供給から唾液腺の除去48時間および72時間後にマウスを配置した後scapularisニンフ。また、成人女性Iから唾液採取を実証scapularisダニ。

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

ダニ媒介性病原体が病気を引き起こす方法感染したダニの体液のコレクション、唾液腺、唾液は勉強することが重要です。このプロトコルでは、餌から体液と唾液腺を収集する方法を示しています<em&gt;イクソデス·スカプラリス</em&gt;ニンフ。また、女性から唾液採取を実証<em&gt; I. scapularis</em&gt;大人。

マダニScapularisダニの唾液、唾液腺、および体液コレクション

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

ダニのRNA干渉

Ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors ...

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

ハイブリダイゼーション<em&gt;その場で</em&gt;唾液腺、卵巣、および媒介蚊の胚の

Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes.  Investigation of transcripts and gene ...

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

ライム病研究における送信および外因診断法のために動物にダニの摂食

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

環境病原体に対するヒトの暴露を測定するために唾液抗体マルチプレックスイムノアッセイの開発

The etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

飼育<em&gt; Ixodes肩甲骨、</em&gt;ブラック・ティック・ティック：未熟期をマウスに与える

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

A Method for the Measurement of Salivary Gland Function in Mice

マウスの唾液腺機能の測定法

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

マウス顎下腺唾液腺の直立した生体顕微鏡検査のための準備

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

唾液圏または単層としての体外増殖のためのヒト唾液腺からの唾液上皮細胞の単離

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

ウイルスから離れた高収率細胞外小胞製剤の精製

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

無脊椎動物の病理学研究に向けた Hemolymph のMetarhizium Blastospores の収集とRhipicephalus Microplusダニへの接種の開示

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...