Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

저자는 벡터 매개로 질환 동물 리소스 지점 부문 감사드립니다, 특히 안드레아 피터슨, 리사 Massoudi, Verna 오브라이언, 그리고 생쥐와 토끼들의 관심과 유지 보수를위한 존 Liddell. 우리는 또한이 원고 향한 그들의 공헌에 대해 에이미 Ullmann, 테레사 러셀, 그리고 바바라 J. 존슨 감사드립니다. 마지막으로, 우리는이 원고의 촬영과 관련된 모든 위법를 찍는 동안 그래픽 삽화와 주디 Lavelle를 생산 CDC에서 커뮤니케이 션을위한 준회원 이사의 사무실에 Alissa Eckert을 인정하고 싶습니다.

1. 슬라이드 준비 Hemolymph 컬렉션 (동영​​상 1) <ol><li> 부드럽게 표면이 멸균을 위해 10 분간 70 % 에탄올에 나서 5 분 3퍼센트 국소 과산화수소의 동물과 장소에서 적극적으로 먹이 진드기를 제거합니다. </li><li> 자궁 펜은 실란 코팅 현미경 슬라이드에 동그라미를 그려와 자궁 펜 서클 내에서 움직이게 장소. <ol><li> 실란 코팅 슬라이드는 현미경 슬라이드에 hemolymph 최고의 준?...

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진드기의 hemolymph, 침샘, 그리고 타액의 컬렉션 진드기 매개로 병원체 전송 연구에 중요하다, 유행, 보급, 확산, 그리고 진드기 및 호스트 6,11-13,20,23,29 모두에서 끈기. 진드기 30,31 해부하는 방법에는 여러 가지가 있습니다. 그러나 침샘을 수집했을 때 제대로 때문에 침샘이 파열이나 진드기의 유골이 안되는 진드기를 해부하는 것이 중요합니다. 침샘들이 midgut 오염을 제거하고 침?...

<table border="1"><tbody><tr><td> 시약 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> 과산화수소 </td><td> 어부 </td><td> H312-500 </td></tr><tr><td> 에탄올 </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> 보스턴 Bioproducts </td><td> BM-2205 </td></tr><tr><td> 뒤몽 파인 포셉 (3C) </td><td> 어부 </td><td> NC9906085 </td></tr><tr><td> 실란 처리 현미경 슬라이드 </td><td> Bioworld </td><td> 42763007-1 </td></tr><tr><td> 자궁 펜 </td><td> Bioworld </td><td> 21750008-1 </td></tr><tr><td> 슈퍼 서리 플러스 현미경 슬라이드 </td><td> 어부 </td><td> 12-550-18 </td></tr><tr><td> Pilocarpine </td><td> 시그마 </td><td> P6503-5G </td></tr><tr><td> 테아제 억제제 칵테일 </td><td> 시그마 </td><td> P2714 </td></tr><tr><td> # 11 메스 일회용 </td><td> 깃털 </td><td> 2975 # 11 </td></tr><tr><td> 독성 모델링 점토 </td><td&gt; 피셔 </td><td> S17307 </td></tr><tr><td> 모세관 튜브 </td><td> 체이스 과학적 유리, INC </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

진드기는 전세계 찾은 많은 진드기 매개로 질병으로 인간을 괴롭히다하고 있습니다. 진드기 라임 병 및 진드기 매개로 회귀열 (Borrelia SPP.), 록키 마운틴 뇌척수 막염 (Rickettsia rickettsii), ehrlichiosis (Ehrlichia chaffeensis와 E 동등), anaplasmosis (Anaplasma phagocytophilum), 뇌염 (똑딱똑딱의 원인 병원체에 대한 벡터입니다 매개 뇌염 바이러스), babesiosis (Babesia SPP.), 콜로라도 진드기 열 (Coltivirus) 및 tularemia (Francisella tularensis) 1-8. 제대로 호스트로 전송하기 위해서는 이러한 전염성 요원들은 differentially, 유전자 발현을 조절 진드기 단백질과 상호 작용하고, 진드기 3,9-13을 통해 마이 그 레이션. 예를 들어, 라임 병 에이전트, Borrelia burgdorferi는 진드기의 enzootic 사이클 14,15의 향연과 기근 단계로 차등 유전자 발현을 통해 적응. 또한, Ixodes의 진드기와 같은가를 소비bloodmeal Borrelia은 복제 및 midgut에서 그들이 침샘을 여행하고 퇴학 타액 9,16-19있는 호스트로 전송될 hemocoel,로 마이 그 레이션. 진드기는 피드로서 호스트는 일반적으로 강한 hemostatic와 타고난 면역 반응 11,13,20-22로 응답합니다. 이러한 호스트 반응에도 불구하고, 전 진드기의 타액은 3,11,20,21,23 먹이 진드기들을위한 immunomodulatory있는 단백질, lytic 대리인, anticoagulants 및 fibrinolysins가 포함되어 있기 때문에 scapularis는 몇 일 동안 먹을 수 있습니다. 진드기의 타액이나 침샘 추출물 (SGE)에 의해 점령 immunomodulatory 활동이 많은 진드기 매개로 병원균 3,20,24-27의 전염, 확산 및 보급을 촉진. 더 이상 그것이 진드기 먹이 적극적으로 해부하고 진드기의 타액을 수집하는 데 필수적이며 전염성 요원들이 질병을 일으킬 방법 진드기 매개로 이해하기 위해서. 이 비디오 프로토콜에 대한 해부 기술을 보여줍니다hemolymph의 수집 및 적극적 나도 먹이로부터 침샘의 제거 scapularis nymphs 48 후 72 시간 포스트 마우스 배치. 우리는 또한 성인 여성 I.에서 타액 수집을 보여줍니다 scapularis 눈금.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

감염된 진드기의 hemolymph, 침샘, 그리고 타액의 컬렉션 병원균이 질병을 일으킬 방법 진드기 매개로 공부하는 것이 중요합니다. 이 프로토콜에서는 먹이로부터 hemolymph과 침샘을 수집하는 방법을 보여줍니다<em&gt; scapularis을 Ixodes</em&gt; nymphs. 우리는 또한 여성에서 타액 수집을 보여줍니다<em&gt; 전 scapularis</em&gt; 성인.

Ixodes Scapularis 진드기의 타액, 침샘 및 Hemolymph 컬렉션

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

진드기의 RNA 간섭

Ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors ...

연구

면역학 및 감염병학

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

이종 교잡<em&gt; 현장에서</em&gt; 침샘, 난소, 그리고 벡터 모기의 태아의

Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes.  Investigation of transcripts and gene ...

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

라임 병 연구의 변속기 및 Xenodiagnosis에 대한 동물에 진드기의 먹이

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

타액 항체 멀티 플렉스 면역을 개발하는 것은 환경 병원체의 인체 노출을 측정하는

The etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

사육<em&gt; Ixodes의 scapularis,</em&gt; 블랙 다리 틱 : 마우스에 미성숙 단계를 먹이

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

A Method for the Measurement of Salivary Gland Function in Mice

쥐에 있는 침 샘 기능의 측정 하는 방법

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

똑바로 Intravital 현미경 Murine Submandibular 침 샘의 준비

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

바이러스로부터 멀리 떨어진 고수율 세포 외 소포 제제의 정제

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

무척 추 병리학 연구를 향한 Rhipicephalus 마이크로 플러스 진드기에 Metarhizium 발 파 포자의 혈 림프 수집 및 접종을 공개

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...