Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Os autores gostariam de agradecer à Divisão de Vector Borne-Doenças Animais Branch Resources, especificamente Andrea Peterson, Lisa Massoudi, Verna O&#39;Brien, e John Liddell para seu cuidado e manutenção dos ratos e coelhos. Gostaríamos também de agradecer a Amy Ullmann, Theresa Russell, e Barbara J. Johnson para suas contribuições para este manuscrito. Finalmente, gostaríamos de reconhecer Alissa Eckert no Gabinete do Diretor Adjunto de Comunicação do CDC para produzir as ilustrações gráficas e Judy Lavelle para dirigir todos os assuntos legais relacionados com a filmagem deste manuscrito.

1. Coleta de hemolinfa para a preparação de slides (Filme 1) <ol><li> Remover suavemente activamente carrapatos de alimentação a partir de um animal e em lugar de peróxido de hidrogénio a 3% tópica durante 5 minutos e, em seguida, em etanol a 70% durante 10 minutos à superfície esterilizar. </li><li> Com uma caneta pap desenhar um círculo sobre uma lâmina de microscópio com revestimento de silano e coloque o carrapato dentro do círculo caneta pap. <ol><li> Silano lâminas reve...

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A coleta de hemolinfa do carrapato, glândulas salivares e saliva é importante no estudo da transmissão de patógenos transmitida por carrapatos, a divulgação, prevalência, proliferação e persistência, tanto carrapato e do exército 6,11-13,20,23,29. Existem várias maneiras de dissecar um carrapato 30,31. No entanto, ao coletar as glândulas salivares é fundamental para dissecar o carrapato corretamente para as glândulas salivares não são rompidas ou perdido em restos do carrapato. Uma...

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saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Carrapatos são encontrados em todo o mundo e afligem os seres humanos com muitas doenças transmitidas por carrapatos. Os carrapatos são vetores de patógenos que causam a doença de Lyme e transmitida por carrapatos febre recorrente (Borrelia spp.), Febre maculosa (Rickettsia rickettsii), erliquiose (Ehrlichia chaffeensis e E. equi), anaplasmose (Anaplasma phagocytophilum), encefalite (tick- vírus da encefalite transmitida), babesiose (Babesia spp.), Colorado febre do carrapato (Coltivirus), e tularemia (Francisella tularensis) 1-8. Para ser adequadamente transmitida para o anfitrião destes agentes infecciosos diferencialmente regulam a expressão gênica, interagir com as proteínas de carrapatos, e migram através do carrapato 3,9-13. Por exemplo, o agente da doença de Lyme, Borrelia burgdorferi, adapta-se através da expressão diferencial de genes para as fases de festa e fome de ciclo enzoótico do carrapato 14,15. Além disso, como carrapatos um Ixodes consome umafarinha de sangue Borrelia replicar e migrar do intestino para a hemocele, onde eles viajam para as glândulas salivares e são transmitidos para o host com a saliva expelida 9,16-19. Como um carrapato se alimenta do hospedeiro geralmente responde com uma resposta hemostática e inata imunológico forte 11,13,20-22. Apesar destas respostas de acolhimento, I. scapularis pode se alimentar por vários dias porque a saliva do carrapato contém proteínas que são imunomoduladores, agentes líticos, anticoagulantes, e fibrinolysins para ajudar o carrapato alimentando 3,11,20,21,23. As atividades imunomoduladoras possuídos pela saliva do carrapato ou extrato de glândula salivar (SGE) facilitar a transmissão, a proliferação e disseminação de inúmeros carrapatos patógenos 3,20,24-27. Para entender melhor como carrapatos agentes infecciosos causam a doença é essencial para dissecar alimentando ativamente carrapatos e coletar a saliva do carrapato. Este protocolo de vídeo demonstra técnicas de dissecação paraa recolha de hemolinfa e à remoção das glândulas salivares de alimentação activamente I. scapularis ninfas após 48 e 72 horas pós posicionamento do mouse. Nós também demonstramos a coleta da saliva de uma fêmea adulta I. carrapato scapularis.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

A coleta de hemolinfa do carrapato infectado, glândulas salivares e saliva é importante estudar como a transmitida por carrapatos patógenos causam doenças. Neste protocolo, demonstramos como coletar glândulas salivares e hemolinfa de alimentação<em&gt; Ixodes scapularis</em&gt; Ninfas. Nós também demonstramos a coleta da saliva da fêmea<em&gt; I. scapularis</em&gt; Adultos.

A saliva, glândula salivar, e Coleta de hemolinfa de carrapatos Ixodes scapularis

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

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Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

RNA de interferência em Carrapatos

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Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

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A Method for the Measurement of Salivary Gland Function in Mice

Um método para a medição da função da glândula salivar em ratos

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Preparação de murino das glândulas salivares Submandibular para microscopia Intravital vertical

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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Isolação de pilhas epithelial salivares das glândulas salivares humanas para o crescimento in vitro como Salispheres ou monolayers

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Purificação de preparações de vesículas extracelulares de alto rendimento longe do vírus

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

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Divulgando a coleta de Hemolinfas e a inoculação de blastósporos de Metarhizium em carrapatos de Rhipicephalus MICROPLUS para estudos de patologia de invertebrados

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...