Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Författarna vill tacka Avdelningen för Vector-Borne Diseases Animal Resources Branch, särskilt Andrea Peterson, Lisa Massoudi, Verna O&#39;Brien, och John Liddell för deras vård och underhåll av möss och kaniner. Vi vill också tacka Amy Ullmann, Theresa Russell, och Barbara J. Johnson för deras bidrag mot detta manuskript. Slutligen vill vi tacka för Alissa Eckert i Office för Associate Director för kommunikation vid CDC för att producera grafiska illustrationer och Judy Lavelle för att koppla alla de juridiska i samband med inspelningen av detta manuskript.

1. Hemolymfa kollektion för preparatet (Film 1) <ol><li> Försiktigt avlägsna aktivt mata fästingar från ett djur och placera i 3% topisk väteperoxid under 5 minuter och sedan i 70% etanol under 10 minuter till ytan sterilisera. </li><li> Med en pap penna rita en cirkel på ett silan belagd objektglas och placera bocken i pap pennan cirkeln. <ol><li> Silan-belagda objektglas användes för bästa vidhäftning av hemolymfa till objektglas. </li></ol></li><li> Visa fästingen under ett ...

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Insamlingen av fästingen hemolymfa, spottkörtlar och saliv är viktig i studiet av fästingburen patogen överföring, förekomst, spridning, spridning och uthållighet i både fästingen och värden 6,11-13,20,23,29. Det finns flera sätt att dissekera en fästing 30,31. Men när samlar spottkörtlarna är det viktigt att dissekera fästingen ordentligt så spottkörtlarna inte spruckit eller förlorade i fästingen kvarlevor. När väl spottkörtlarna avlägsnas från fästingen de måste tvät...

<table border="1"><tbody><tr><td> Reagens </td><td> Företaget </td><td> Katalognr </td></tr><tr><td> Väteperoxid </td><td> Fisher </td><td> H312-500 </td></tr><tr><td> Etanol </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> Boston Bioproducts </td><td> BM-2205 </td></tr><tr><td> Dumont fin pincett (3C) </td><td> Fisher </td><td> NC9906085 </td></tr><tr><td> Silanbehandlade mikroskopobjektglas </td><td> BioWorld </td><td> 42.763.007-1 </td></tr><tr><td> Pap penna </td><td> BioWorld </td><td> 21.750.008-1 </td></tr><tr><td> Super frost plus objektglas </td><td> Fisher </td><td> 12-550-18 </td></tr><tr><td> Pilokarpin </td><td> Sigma </td><td> P6503-5G </td></tr><tr><td> Proteasinhibitorcocktail </td><td> Sigma </td><td> P2714 </td></tr><tr><td> # 11 disponibla skalpell </td><td> Feather </td><td> 2975 # 11 </td></tr><tr><td> Giftfritt modellera </td><td&gt; Fisher </td><td> S17307 </td></tr><tr><td> Kapillärrören </td><td> Chase vetenskapliga Glas, Inc </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Fästingar finns i hela världen och drabbar människor med många fästingburna sjukdomar. Fästingar är vektorer för patogener som orsakar Lyme sjukdom och fästingburen återfallsfeber (Borrelia spp.)., Rocky Mountain spotted fever (Rickettsia rickettsii), ehrlichios (Ehrlichia chaffeensis och E. equi), anaplasmos (Anaplasma phagocytophilum), encefalit (tick- borne encephalitis virus), babesios (Babesia spp.)., Colorado tick feber (Coltivirus) och tularemi (Francisella tularensis) 1-8. Att bli ordentligt överföras till den mottagande dessa infektiösa agens reglerar differentiellt genuttryck, interagera med tick proteiner och migrerar genom fästingen 3,9-13. Till exempel anpassar Lyme smittämnet, Borrelia burgdorferi, genom ojämn genuttryck för att festen och hungersnöd stadier av fästingens enzootisk cykel 14,15. Dessutom förbrukar som Ixodes tick enblodmjöl Borrelia replikera och migrera från tarmkanalen till hemocoel, där de reser till spottkörtlarna och överförs till värden med ut saliven 9,16-19. Som en fästing matar värden svarar vanligen med en stark hemostatiska och medfödda immunsvar 11,13,20-22. Trots dessa värdsvar, I. scapularis kan mata flera dagar eftersom fästingen saliv innehåller proteiner som är immunmodulerande, lytiska medel, antikoagulantia och fibrinolysins att hjälpa fästingen utfodring 3,11,20,21,23. De immunmodulerande aktiviteter innehas av tick saliv eller salivkörtelextrakt (SGE) underlätta överföring, spridning och spridning av många fästingburna patogener 3,20,24-27. För att ytterligare förstå hur fästingburen smittämnen orsaka sjukdom är det viktigt att dissekera aktivt utfodring fästingar och samla fästing saliv. Denna video protokoll visar dissekering tekniker förinsamling av hemolymfa och avlägsnande av spottkörtlarna från att aktivt mata I. scapularis nymfer efter 48 och 72 timmar efter mus placering. Vi visar också att saliv samling från en vuxen hona I. scapularis fästing.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Insamlingen av infekterade fästingar hemolymfa, spottkörtlar och saliv är viktigt att studera hur fästingburen patogener orsaka sjukdom. I detta protokoll visar vi hur man samlar in hemolymfa och spottkörtlar matas<em&gt; Ixodes scapularis</em&gt; Nymfer. Vi visar också att saliven samling från kvinnliga<em&gt; I. scapularis</em&gt; Vuxna.

Saliv, spottkörtel och hemolymfa Collection från Ixodes scapularis fästingar

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

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Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

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Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

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Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

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Rening av högavkastande extracellulära vesikel preparat bort från virus

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Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...