Name: saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks
Uploaded: 2012-02-21T13:00:00
Description: Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Yazarlar vektör kaynaklı hastalıkların Hayvan Kaynakları Şubesi Bölümü teşekkür etmek istiyorum, özellikle Andrea Peterson, Lisa Massoudi, Elçin O&#39;Brien ve fare ve tavşan bakımı ve bakım için John Liddell. Biz de bu yazının doğru katkıları için Amy Ullmann, Theresa Russell, ve Barbara J. Johnson teşekkür etmek istiyorum. Son olarak, bu yazının filme ile ilgili tüm legalities yönlendirmek için grafik çizimler ve Judy Lavelle üretmek için CDC, İletişim için Associate Direktörü Office Alissa Eckert kabul etmek istiyorum.

1. Slayt hazırlanması için hemolimf toplama (Film 1) <ol><li> Yavaşça yüzeyi sterilize ila 10 dakika süreyle% 70 etanol içinde daha sonra 5 dakika boyunca% 3 topikal hidrojen peroksit ve bir hayvan yerden aktif besleme kenelerin kaldırmak ve. </li><li> Pap kalem silan kaplı mikroskop lamı üzerine bir daire çizin ve pap kalem daire içinde kene yerleştirin ile. <ol><li> Silan kaplı slaytlar mikroskop lamı için hemolimf en iyi yapışması için kullanılır. </li></ol></li>...

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Kene hemolimf, tükrük bezleri ve tükürük toplama kene kaynaklı patojen bulaşma çalışmada önemlidir, yaygınlık, yaygınlaştırma, proliferasyon ve kene ve ana 6,11-13,20,23,29 hem de kalıcılık. Bir kene 30,31 incelemek için çeşitli yollar vardır. Ancak, tükrük bezleri alırken düzgün böylece tükürük bezlerinin yırtılması veya kenenin kalıntıların kayıp değildir kene incelemek için önemlidir. Tükürük bezleri onlar ortabağırsak kontaminasyonu gidermek için ...

<table border="1"><tbody><tr><td> Reaktif </td><td> Şirket </td><td> Katalog Numarası </td></tr><tr><td> Hidrojen peroksit </td><td> Balıkçı </td><td> H312-500 </td></tr><tr><td> Etanol </td><td> Acros </td><td> 61509-5000 </td></tr><tr><td> PBS </td><td> Boston Bioproducts </td><td> BM-2205 </td></tr><tr><td> Dumont Güzel forseps (3C) </td><td> Balıkçı </td><td> NC9906085 </td></tr><tr><td> Silan tedavi mikroskop </td><td> Bioworld </td><td> 42763007-1 </td></tr><tr><td> Pap kalem </td><td> Bioworld </td><td> 21750008-1 </td></tr><tr><td> Süper don artı mikroskop </td><td> Balıkçı </td><td> 12-550-18 </td></tr><tr><td> Pilokarpin </td><td> Sigma </td><td> P6503-5G </td></tr><tr><td> Proteaz inhibitörü kokteyl </td><td> Sigma </td><td> P2714 </td></tr><tr><td> # 11 bistüri atılabilir </td><td> Tüy </td><td> 2975 # 11 </td></tr><tr><td> Zehirli kil modelleme </td><td&gt; Fisher </td><td> S17307 </td></tr><tr><td> Kapiler tüpler </td><td> Chase bilimsel Cam, inc </td><td> 40A502 </td></tr></tbody></table>

saliva, salivary gland, and hemolymph collection from ixodes scapularis ticks

Keneler dünya çapında bulunur ve birçok kene kaynaklı hastalıklar insanlarda eziyet edilir. Keneler, Lyme hastalığı ve kene kaynaklı tekrarlayan ateş (Borrelia spp.), Rocky Mountain Benekli Ateşi (Rickettsia rickettsii), Ehrlichiosis (Ehrlichia chaffeensis ve E. equi), anaplazmoz (Anaplasma phagocytophilum), ansefalit (kene neden patojenler için vektörleri kaynaklı ensefalit virüsü), babesiosis (Babesia spp.), Kolorado kene ateşi (Coltivirus) ve tularemi (Francisella tularensis) 1-8. Düzgün konak içine aktarılacak bu enfeksiyon ajanları farklı şekilde, gen ekspresyonu düzenleyen kene proteinleri ile etkileşim ve kene 3,9-13 yoluyla göç ederler. Örneğin, Lyme hastalığı ajanı, Borrelia burgdorferi, kenenin enzootik döngüsü 14,15 şölen ve kıtlık aşamalarına farklı gen ifade aracılığıyla uyarlar. Dahası, bir Ixodes kene gibi bir tüketirbloodmeal Borrelia çoğaltmak ve ortabağırsak gelen onlar tükürük bezlerinin seyahat ve sınırdışı tükürük 9,16-19 ile ev sahibi içine iletilir hemocoel, göç. Kene beslenirken ev sahibi genellikle güçlü bir hemostatik ve immün yanıtı 11,13,20-22 ile yanıt verir. Bu konak yanıtları rağmen, I. kene tükürük 3,11,20,21,23 besleyen kene yardım immünomodülatör olan proteinlerin parçalayıcı ajanlar, antikoagülanlar ve fibrinolysins içerdiğinden scapularis birkaç gün süreyle besleyebilir. Kene tükürük veya tükürük bezi ekstraktı (SGE) tarafından sahip immünomodülatör faaliyetleri çok sayıda kene kaynaklı patojenlerin 3,20,24-27 iletimi, çoğalması ve yayılması kolaylaştırmak. Daha da kenelerin beslenme aktif incelemek ve kene tükürük toplamak esastır enfeksiyöz ajanlar hastalığı nasıl neden kene kaynaklı anlamak için. Bu video protokol için diseksiyon teknikleri gösteriyorhemolimf toplanması ve aktif I. beslenmesini tükürük bezlerinin çıkarılması scapularis periler 48 sonra ve 72 saat sonrası fare yerleşimi. Ayrıca yetişkin bir dişi I. tükürük toplama göstermek scapularis kene.

Watch this Scientific Journal Video about Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks at JoVE.com

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

Keneler hemolimf, tükrük bezleri ve tükürük toplama patojenler hastalıklara nasıl neden kene kaynaklı incelemek önemlidir. Bu protokolü biz beslenmesini hemolimf ve tükrük bezleri toplamak nasıl gösterilmektedir<em&gt; Scapularis Ixodes</em&gt; Periler. Ayrıca kadın tükürük toplama göstermek<em&gt; I. scapularis</em&gt; Yetişkinler.

Ixodes scapularis Keneler gelen tükürük, Tükürük Bezi ve hemolimf Koleksiyonu

Ticks are found worldwide and afflict humans with many tick-borne illnesses. Ticks are vectors for pathogens that cause Lyme disease and tick-borne ...

Research

JoVE Journal

Immunology and Infection

Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks

RNA Interference in Ticks

Keneler de RNA Girişim

Ticks are obligate hematophagous ectoparasites of wild and domestic animals and humans, and are considered to be second worldwide to mosquitoes as vectors ...

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes

Melezleme<em&gt; In situ</em&gt; Bezler, Yumurtalık ve Vektör Sivrisinek ve Embriyoların

Mosquitoes are vectors for a diverse set of pathogens including arboviruses, protozoan parasites and nematodes.  Investigation of transcripts and gene ...

Feeding of Ticks on Animals for Transmission and Xenodiagnosis in Lyme Disease Research

Lyme Hastalığı Araştırma İletim ve Xenodiagnosis için Hayvanlar Keneler besleme

Transmission of the etiologic agent of Lyme disease, Borrelia burgdorferi, occurs by the  attachment and blood feeding of Ixodes species ticks on ...

Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens

Bir Tükürük Antikor Multiplex Immuoassay Gelişmekte Çevre Patojenler İnsan Pozlama&#39;yı Tedbir

The etiology and impacts of human exposure to environmental pathogens are of major concern worldwide and, thus, the ability to assess exposure and ...

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

Rearing Ixodes scapularis, the Black-legged Tick: Feeding Immature Stages on Mice

yetiştirme<em&gt; Geyik kenesi,</em&gt; Siyah Ayaklı Tik Tak: Fare üzerinde Olgunlaşmamış Evreleri Besleme

Ixodes scapularis, the vector of Lyme disease, is one of the most important disease vectors in the eastern and Midwestern United States. This species is a ...

A Method for the Measurement of Salivary Gland Function in Mice

Tükürük bezi işlevi farelerde ölçülmesi için bir yöntem

Patients with Sj&#246;gren&#39;s syndrome, an autoimmune disease affecting the exocrine glands, develop salivary gland inflammation and have reduced ...

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Dik Intravital mikroskopi için fare Submandibular Tükürük bezi hazırlanması

The submandibular salivary gland (SMG) is one of the three major salivary glands, and is of interest for many different fields of biological research, ...

Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers

Salispheres veya Monolayers olarak In vitro büyüme için ınsan tükürük bezlerinden tükürük epitelyal hücrelerin yalıtım

The salivary glands are a site of significant interest for researchers interested in multiple aspects of human disease. One goal of researchers is to ...

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Yüksek Verim Ekstrasellüler Vezikül Preparatlarının Virüsten Uzaklaştırılması

One of the major hurdles in the field of extracellular vesicle (EV) research today is the ability to achieve purified EV preparations in a viral infection ...

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Hemolymph toplama ve Metarhizyum blastosporların omurgasız patoloji çalışmalarına doğru Rhipicephalus Microplus ticks içine inoculation

Ticks are obligate hematophagous ectoparasites and Rhipicephalus microplus has great importance in veterinary medicine because it causes anemia, weight ...