Name: a murine closed-chest model of myocardial ischemia and reperfusion
Uploaded: 2012-07-17T12:00:00
Description: Watch this Scientific Journal Video about A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion at JoVE.com

我们感谢丹尼尔迪埃尔灌注固定技术为他的意见。

1。麻醉诱导<ol><li>异氟醚诱导，鼠标放到感应框，它是连接到蒸汽集到3.0％和0.5 L / min的氧流量。 </li><li>无意识与触觉的刺激，未能诱发反应和前肢或后肢踏板撤回反射缺席实现后，放置在一个温度控制的手术台鼠标在仰卧位。鼻锥通过感应框连接到蒸汽维持麻醉。放倒头固定用5-0尼龙线的上门牙，以方便插管。 </li><li>插入直肠温度探头的身体核心温度保持在37°C。用胶带固定四肢。越过较低?...

<ol>
	<li>Lim, S. Y., Davidson, S. M., Hausenloy, D. J., Yellon, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preconditioning+and+postconditioning:+the+essential+role+of+the+mitochondrial+permeability+transition+pore.">Preconditioning and postconditioning: the essential role of the mitochondrial permeability transition pore.</a> Cardiovasc. Res. 75, 530-535 (2007).</li><li>Eckle, T., Koeppen, M., Eltzschig, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+a+Hanging+Weight+System+for+Coronary+Artery+Occlusion+in+Mice.">Use of a Hanging Weight System for Coronary Artery Occlusion in Mice.</a> J. Vis. Exp. (50), e2526 (2011).</li><li>Michael, L. 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我们已经修改通过访问通过左侧肋间开胸的心脏和领导法援署缝线，在左锁骨中线胸部封闭胸小鼠模型。保持骨左肋不变，将最大限度地减少创伤，需要止痛药物，外科手术部位感染，从而，促进恢复。通过保留左动脉内部哺乳动物有没有电灼需要。我们离开以后方便缝合皮下组织循环及使用吊重系统定义的闭塞。胸外模型使心肌缺血再灌注的所有研究方案的应用与手术创伤和随后的免疫反应<sup...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>汽</td><td> drägerwerk公司</td><td> isoflo </td></tr><tr><td>显微镜</td><td>徕卡</td><td> M80的</td></tr><tr><td>光源</td><td>肖特</td><td>吉隆坡1500液晶</td></tr><tr><td> homeothermic毯控制单元</td><td>哈佛仪器</td><td></td></tr><tr><td> MiniVent类型845 </td><td> HUGO高盛Elektronik公司</td><td></td></tr><tr><td> 8-0普理灵</td><td>爱惜康</td><td> BV130-56.5毫米3/8c </td></tr><tr><td> 6-0普理灵</td><td>爱惜康</td><td> BV-19.3毫米3/8c </td></tr><tr><td> kalt缝合针大小为3 </td><td>福斯特</td><td> 12050-03 </td></tr><tr><td>三苯</td><td> Sigma Aldrich公司</td><td> 93145 </td&gt; </tr><tr><td>酞菁蓝</td><td> Heubach GmbH德国有限公司</td><td></td></tr><tr><td> PowerLab系统</td><td> ADInstruments </td><td></td></tr></tbody></table>

a murine closed-chest model of myocardial ischemia and reperfusion

开胸冠状动脉结扎模型开胸手术创伤修改不同的机制参与缺血再灌注诱导的免疫反应。免疫反应包括细胞因子的表达和释放或分泌的内源性配体的先天免疫受体。先天免疫系统的激活可能调节心肌梗死面积。我们已经修改现有的小鼠封闭胸部模型，使用吊重研究心肌预处理和后处理和先天免疫在心肌缺血再灌注损伤中的作用，这可能是有用的。这种模式使动物心肌缺血的发作前从手术创伤恢复。 挥发性麻醉药已激烈的研究和预处理缺血心脏的影响是众所周知的。然而，这种保护作用排除在开胸冠状动脉结扎模型，它的使用。因此，另一个优势是次使用Ë以及可控的挥发性麻醉药仪器在慢性闭胸模型，因为他们的预处理效果持续长达72小时。慢性心脏疾病，间歇性缺血，多打款是这种模式的其他可能的应用。 为慢性闭胸模型，气管插管和通风的小鼠接受外侧钝通过的第4肋间开胸。继左前降连字前的鉴定，通过下面的船只都缝合两端螺纹通过封堵。然后，两个缝合两端都通过胸壁，打结，形成一个循环，并留在皮下组织。胸部关闭和恢复为5天之后，老鼠再次麻醉，胸部皮肤被重新吊重迷上了心电图控制下的循环。 在缺血/再灌注协议结束时，心中可以与TTC染色的梗塞大小评估或进行灌注固定，允许​​除了组织学和免疫组化形态的研究。

Watch this Scientific Journal Video about A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion at JoVE.com

A Murine Closed-chest Model of Myocardial Ischemia and Reperfusion

手术创伤引起的炎症反应。被称为细胞因子和内源性配体调节缺血再灌注后心肌梗死面积。我们提出了小鼠缺血及使用吊重的再灌注，以减少开胸手术的效果修改封闭胸部模型。

闭胸鼠心肌缺血再灌注模型

Surgical trauma by thoracotomy in open-chest models of coronary ligation induces an immune response which modifies different mechanisms involved in ...

Research

JoVE Journal

Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

常温心脏骤停和心肺复苏术：缺血再灌注损伤的小鼠模型

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

科研

医学

Reproducable Paraplegia by Thoracic Aortic Occlusion in a Murine Model of Spinal Cord Ischemia-reperfusion

可重放截瘫胸主动脉阻断脊髓缺血再灌注的小鼠模型

Background
Lower extremity paralysis continues to complicate aortic interventions. The lack of understanding of the underlying pathology has hindered ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

心肌缺血再灌注损伤的通过左前降支结扎小鼠模型

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

A Rat Model of Ventricular Fibrillation and Resuscitation by Conventional Closed-chest Technique

室颤由传统的封闭胸技术大鼠模型和复苏

A rat model of electrically-induced ventricular fibrillation followed by cardiac resuscitation using a closed chest technique that incorporates the basic ...

Murine Model of Intestinal Ischemia-reperfusion Injury

肠缺血再灌注损伤的小鼠模型

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Confirmation of Myocardial Ischemia and Reperfusion Injury in Mice Using Surface Pad Electrocardiography

小鼠使用表面垫心电图心肌缺血再灌注损伤的确认

Many animal models have been established for the study of myocardial remodeling and heart failure due to its status as the number one cause of mortality ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

组织和采血程序标准化改善闭合性胸猪心肌梗死模型的研究

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

A Closed-chest Model to Induce Transverse Aortic Constriction in Mice

诱导小鼠横向主动脉狭窄的闭合胸模型

Research on cardiac hypertrophy and heart failure is frequently based on pressure overload mouse models induced by TAC. The standard procedure is to ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

在穆林模型中缺血再灌注损伤后干细胞的心肌内传递延迟

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...