Name: do-it-yourself device for recovery of cryopreserved samples accidentally dropped into cryogenic storage tanks
Uploaded: 2012-05-11T13:00:00
Description: Watch this Scientific Journal Video about Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks at JoVE.com

“在真核细胞中，利用生物信息学和分子生物学方法的建模细菌蛋白质异戊烯”玛丽·路易丝·安德鲁斯奖的癌症研究计划，弗吉尼亚州科学院主办，“血浆中的RNA编辑的生理意义的项目过程中，DIY低温检索设备已测试树突状细胞“由托马斯·Jeffress和凯特·米勒Jeffress纪念信托和国家合同16.740.11.0364（科教部，俄罗斯）。我们想感谢援助与RNA的完整性实验贝丝EOM。

1。大会Cryotolerant设备Cryoboxes检索<ol><li>用钳子，理顺3片面强领带（ 图1A），使L形图1b所示的强领带注：侧。强领带的尺寸可以选择取决于杜瓦颈部直径。 </li><li>确保两个这样的强强烈的领带ţ表带使用皇冠螺栓螺母，垫圈和螺钉形成Cryoscoop基地，在图3（AB）所示（ 图2）注：两强关系是用来允许检索5年半英寸x 5½英寸cryoboxes。 </li>...

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	<li>Kittel, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Advances in Cryogenic Engineering. , 41 (1996).</li><li>Edeskuty, F. J., Walter, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Stewart Safety in the handling of cryogenic fluids. , (1996).</li><li>Council, N. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Prudent Practices in the Laboratory: Handling and Management of Chemical Hazards. , (2011).</li><li>Muyal, J., Muyal, V., Kaistha, B., Seifart, C., Fehrenbach, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+comparison+of+RNA+extraction+techniques+from+frozen+and+fresh+lung+tissues:+checkpoint+towards+gene+expression+studies.">Systematic comparison of RNA extraction techniques from frozen and fresh lung tissues: checkpoint towards gene expression studies.</a> Diagnostic Pathology. 4, 9 (2009).</li><li>Sambrook, J., Russell, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Molecular cloning: a laboratory manual. , (2001).</li><li>Holland, N. T., Smith, M. T., Eskenazi, B., Bastaki, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+sample+collection+and+processing+for+molecular+epidemiological+studies.">Biological sample collection and processing for molecular epidemiological studies.</a> Mutation Research/Reviews in Mutation Research. 543, 217-234 (2003).</li><li>Lynch, P. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=CELL,+TISSUE+AND+ORGAN+CULTURE+|+Storage+and+Cryopreservation.">CELL, TISSUE AND ORGAN CULTURE | Storage and Cryopreservation.</a> Encyclopedia of Rose Science. , 106-111 (2003).</li><li>Crawford, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Plastics Engineering. , (1998).</li><li>Gorenc, B., Tinyou, R., Syam, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Steel designers' handbook. , (1996).</li><li>Harper, C. A., Petrie, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Plastics+materials+and+processes:+a+concise+encyclopedia.">Plastics materials and processes: a concise encyclopedia.</a> Wiley-Interscience. , (2003).</li></ol>

检索cryoboxes和冷冻管从杜瓦罐底部的最常用的方法之一是迁置到一个备用的容器中的液态氮和拉出样品留在烧瓶或漂浮在倒出的液氮。然而，这是一个不安全的做法，可能会导致低温烫伤或窒息，由于长时间暴露在液氮蒸汽2。其他常见的方式检索堕落的冷冻管涉及使用离心管钳3。然而，黑暗的空间内充满液氮罐的能见度有限，限制了其可操作性。 如上所述?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>希尔曼集团镀开槽平1-3/8×4英尺14计</td><td>当地的五金商店</td><td> N / A </td><td> 1个单位的要求</td></tr><tr><td>皇冠螺栓不锈钢＃6 - 32×3/8英寸扁圆头十字径机螺杆</td><td>当地的五金商店</td><td>型号＃42281 </td><td>需要4个单位</td></tr><tr><td>冠螺栓3/8英寸x 1-1/2英寸镀锌钢护舷垫圈</td><td>当地的五金商店</td><td>型号＃20122 </td><td>需要4个单位</td></tr><tr><td>冠螺栓锌镀＃8-32机螺杆螺帽</td><td>当地的五金商店</td><td>型号＃18512 </td><td>需要4个单位</td></tr><tr><td>辛普森强铁BC40Z 4英寸半基地In.x4 </td><td>本地硬件购物</td><td>型号＃BC40Z </td><td>需要2个单位</td></tr><tr><td>辛普森强铁6英寸x 6英寸ţ表带</td><td>当地的五金商店</td><td>型号：66T </td><td> 1个单位的要求</td></tr><tr><td>奥奈达6“滤网</td><td>本地杂货店</td><td>型号：7859W </td><td> 1个单位的要求</td></tr><tr><td>该的希尔曼集团镀锌六角螺母3/8-16 </td><td>当地的五金商店</td><td>型号＃：810509 </td><td>需要6个单位</td></tr><tr><td>该的希尔曼集团镀锌六角螺栓3/8- 16 </td><td>当地的五金商店</td><td></td><td>需要4个单位</td></tr><tr><td>绝缘泡沫</td><td>当地的五金商店</td><td></td><td> 1个单位的要求</td></tr><tr><td>螺杆驱动程序</td><td>当地的五金商店</td><td></td><td> 1个单位的要求</td></tr><tr><td>钳子</td><td>当地的五金商店</td><td></td><td> 1个单位的要求</td></tr></tbody></table>

do-it-yourself device for recovery of cryopreserved samples accidentally dropped into cryogenic storage tanks

液氮是无色，无嗅，极冷（-196℃）的液体在压力下保持。它通常被用来作为一个长期的生物材料，如血液，细胞和组织1,2长期储存的低温液体。液氮低温性质，而理想的样品保存，可引起接触活组织快速冷冻-被称为“低温烧伤”2，这可能会导致密切合作，参与从杜瓦瓶样品的存储和检索的人严重冻伤。此外，液氮蒸发，降低空气中的氧气浓度，并可能导致窒息，特别是在密闭空间2。 在实验室，生物样品往往存储在冷冻管或不锈钢架堆放在杜瓦罐1 cryoboxes。提供这些存储架，以防止打滑从机架盒与长轴成底部杜瓦在常规处理。然而，过于频繁，箱或珍贵样品小瓶溜出去和充满液氮罐的底部下沉。在这种情况下，样本可以不厌其烦地检索后液氮转移到一个备用的容器中或丢弃它。然后可以相对安全地从回收空杜瓦盒和瓶。然而，低温液态氮，其扩张速度的性质，使沉船样本检索的危险。它通常被推荐样本检索从未由一个人进行了安全处。另一种方法是使用市售的酷抓斗或钳拉出瓶3。在黑暗的充满液体的杜瓦瓶的能见度有限，但在其使用带来的一个主要的限制。 在这篇文章中，我们描述了一个Cryotolerant DIY的检索设备，这使得从杜瓦样本检索含有低温液体既安全建设第二容易。

Watch this Scientific Journal Video about Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks at JoVE.com

Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks

在这里，我们提出了成本低，耐用cryotolerant设备样品从充满液氮的杜瓦罐检索。易于施工和设备的模块化设计使得低温储罐的样本检索过程的安全和方便。

做自己动手恢复冻存样品的设备不慎掉落到低温储罐

Liquid nitrogen is colorless, odorless, extremely cold (-196 &deg;C) liquid kept under pressure. It is commonly used as a cryogenic fluid for long term ...

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