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Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)

14.0K Views

11:54 min

April 29th, 2012

DOI :

10.3791/3918-v

April 29th, 2012


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Slow dividing Cells

Chapters in this video

0:02

Title

0:44

Introduction

1:43

Preparing Glioblastoma Single Cell Suspension

3:06

Staining with Cell Trace Carboxyfluorescein Succinimidyl Ester (CFSE) Green Fluorescent Dye

5:37

Flow Cytometry Analysis of CFSE Stained Glioblastoma Cells

6:44

Isolation of the Slow-dividing Cell Population using Fluorescent Activated Cell Sorting (FACS)

10:02

Representative Results

11:12

Conclusion

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