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Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

DOI :

10.3791/54259-v

7:10 min

July 29th, 2016

July 29th, 2016

12,224 Views

1R&D Reagents, Miltenyi Biotec GmbH, 2In vivo Tumorbiology, Oncotest GmbH

Human tumor xenografts are vascularized and infiltrated by cells of mouse origin during the growth phase in vivo. To circumvent the bias caused by these contaminating cells during downstream analysis, we developed a method allowing for comprehensive depletion of all mouse cells by magnetic cell sorting.

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Depletion

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