Name: electroantennographic bioassay as a screening tool for host plant volatiles
Uploaded: 2012-05-06T14:00:00
Description: Watch this Scientific Journal Video about Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles at JoVE.com

Данное исследование было проведено при USDA-ARS КРИС проекта 5325-42000-037-00D и с результатами CRADA 58-3K95-7-1198 и TFCA 58-5325-8-419. Авторы выражают благодарность Suterra за дар (Z, Z) -11,13-hexadecadienal, Б. Хигби для продуктивного обсуждения и Дж. Бейкер по оказанию технической помощи.

1. Подготовка Летучие обнаружен с растением-хозяином для скрининга ЕАГ <ol><li> После соответствующей идентификации и аутентификации всех летучих веществ с помощью GC-MS, выполнить ЕАГ слоеного анализ каждого из доступных нестабильной. Первоначальная проверка может быть небольшое количе?...

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Использование electroantennogram записи как биопробы, чтобы определить хеморецепции ответы целевой насекомых является довольно распространенным и многочисленных исследований с использованием ЕАГ в качестве детектора для стоков из газовой хроматограмме (GC-EAD) можно найти в литературе. 9,10</s...

<table border="1"><tbody><tr><td> Название реагента </td><td> Компания </td><td> Номер по каталогу </td><td> Комментарии </td></tr><tr><td> Ацетофенона </td><td> Альфа-Aesar </td><td> A12727 </td><td> Женский положительный контроль </td></tr><tr><td> (Z, Z) -11,13-Hexadecadienal </td><td> Suterra </td><td></td><td> Мужской положительный контроль </td></tr><tr><td> α-Humulene </td><td> Aldrich </td><td> 53675 </td><td> Сесквитерпеновые </td></tr><tr><td> 2-Undecanone </td><td> Aldrich </td><td> U1303 </td><td> Жирные кислоты, производные </td></tr><tr><td> 2-фенилэтанола </td><td> Aldrich </td><td> 77861 </td><td> Бензеноидных </td></tr><tr><td> Пентан </td><td> EMD </td><td> PX0167-1 </td><td> Растворитель </td></tr><tr><td> 4-канальный контроллер приобретение </td><td> Syntech </td><td> ЯDAC-4 </td><td></td></tr><tr><td> ЕАГ зонд, предварительного усилителя </td><td> Syntech </td><td> Тип PRG-2 </td><td></td></tr><tr><td> Антенна держатель </td><td> Syntech </td><td> Для PRG-2 </td><td> Вилка электрод </td></tr><tr><td> Стимул контроллер </td><td> Syntech </td><td> CS-55 </td><td> Расход воздуха и клубы </td></tr><tr><td> Гель спектры электродов </td><td> Паркер </td><td> 12-02 </td><td></td></tr><tr><td> Биопроб дисков </td><td> Ватман </td><td> 2017-006 </td><td> 6 мм </td></tr><tr><td> Пастера пипец </td><td> VWR </td><td> 14673-010 </td><td> 5 ¾ &quot;(14,6 см) </td></tr><tr><td> Парафильмом M </td><td> Bemis </td><td> PM-992 </td><td></td></tr></tbody></table>

electroantennographic bioassay as a screening tool for host plant volatiles

Plant volatiles play an important role in plant-insect interactions. Herbivorous insects use plant volatiles, known as kairomones, to locate their host plant.1,2 When a host plant is an important agronomic commodity feeding damage by insect pests can inflict serious economic losses to growers. Accordingly, kairomones can be used as attractants to lure or confuse these insects and, thus, offer an environmentally friendly alternative to pesticides for insect control.3 Unfortunately, plants can emit a vast number volatiles with varying compositions and ratios of emissions dependent upon the phenology of the commodity or the time of day. This makes identification of biologically active components or blends of volatile components an arduous process. To help identify the bioactive components of host plant volatile emissions we employ the laboratory-based screening bioassay electroantennography (EAG). EAG is an effective tool to evaluate and record electrophysiologically the olfactory responses of an insect via their antennal receptors. The EAG screening process can help reduce the number of volatiles tested to identify promising bioactive components. However, EAG bioassays only provide information about activation of receptors. It does not provide information about the type of insect behavior the compound elicits; which could be as an attractant, repellent or other type of behavioral response. Volatiles eliciting a significant response by EAG, relative to an appropriate positive control, are typically taken on to further testing of behavioral responses of the insect pest. The experimental design presented will detail the methodology employed to screen almond-based host plant volatiles4,5 by measurement of the electrophysiological antennal responses of an adult insect pest navel orangeworm (Amyelois transitella) to single components and simple blends of components via EAG bioassay. The method utilizes two excised antennae placed across a "fork" electrode holder. The protocol demonstrated here presents a rapid, high-throughput standardized method for screening volatiles. Each volatile is at a set, constant amount as to standardize the stimulus level and thus allow antennal responses to be indicative of the relative chemoreceptivity. The negative control helps eliminate the electrophysiological response to both residual solvent and mechanical force of the puff. The positive control (in this instance acetophenone) is a single compound that has elicited a consistent response from male and female navel orangeworm (NOW) moth. An additional semiochemical standard that provides consistent response and is used for bioassay studies with the male NOW moth is (Z,Z)-11,13-hexdecadienal, an aldehyde component from the female-produced sex pheromone.6-8

Watch this Scientific Journal Video about Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles at JoVE.com

Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles

Метод быстро экран летучие растения-хозяина путем измерения электрофизиологических ответ взрослого orangeworm пупка (<em&gt; Amyelois transitella</em&gt;) Антенны на отдельные компоненты и смеси через electroantennographic анализ показал.

Electroantennographic биопроб в качестве скринингового инструмента для летучих растения-хозяина

Plant volatiles play an important role in plant-insect interactions. Herbivorous insects use plant volatiles, known as kairomones, to locate their host ...

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