Name: clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)
Uploaded: 2012-03-17T14:00:00
Description: Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

LT har modtaget tilskud fra den Forschungsförderungsprogramm af universitetet Medicine Göttingen. PL og MB blev støttet af DFG Research Center for Molekylær Fysiologi af hjernen (CMPB), Göttingen. Forfatterne takker Dr. Lars Tatenhorst for hjælp med videography og Birgit Liebau om hjælp med lyd og video redigering.

Dyrene blev indkøbt fra Jackson Laboratory (# 002.726) 1. De er klinisk scorede og underkastes en test af motorisk funktion (rotarod test) og muskelstyrke (hængende wire test). Alle disse test og senere aflivningen af ​​dyr med henblik på at forberede rygmarven er blevet udført i meget tæt overensstemmelse med de lokale retningslinjer for korrekt afvikling af dyreforsøg. 1. Klinisk score Bortset fra vurdering til en kropsvægt på mus undersøg...

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Den SOD1 (G93A) genetisk musemodellen er et værdifuldt dyremodel til undersøgelse af sygdommens forløb fremadskridende motoneuron tab sammenlignes med human amyotrofisk lateral sklerose 8. En række forskellige behandlingsgrupper paradigmer er blevet evalueret i denne model og udgør grundlaget for senere teste i humane kliniske undersøgelser 8-10. For at være i stand til at detektere signifikante forskelle i en eksperimentel behandling undersøgelse i disse mus, er det yderst vigtigt at indbe...

<table border="1"><tbody><tr><td></td><td> Producent </td><td> Bestillingsinformation </td></tr><tr><td> Adfærdsmæssig testning </td><td></td><td></td></tr><tr><td> Rota-Rod for mus </td><td> Ugo Basile, Comerio, Italien </td><td> # 47600 </td></tr><tr><td> Hængende wire enhed </td><td> Skræddersyet </td><td></td></tr><tr><td colspan="3"></td></tr><tr><td> Spinal Cord Forberedelse </td><td></td><td></td></tr><tr><td> Operationsbordet Operation lampe Beskyttelseshandsker </td><td></td><td></td></tr><tr><td> &quot;Iris&quot; Sakse, vinklet til side </td><td> Fin Science Tools, Heidelberg, Tyskland </td><td> 14063-09 </td></tr><tr><td> Cohan-Vännäs Spring saks, lige </td><td> Fin Science Tools, Heidelberg, Tyskland </td><td> 15000-10 </td&gt; </tr><tr><td> Micro tang </td><td> Hammacher, Solingen, Tyskland </td><td> HWC 111-10 </td></tr><tr><td> Skalpel &quot;präzisa plus&quot; </td><td> Dahlhausen, Köln, Tyskland </td><td> 11.000.00.510, fig 10 </td></tr></tbody></table>

clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)

Amyotrofisk lateral sklerose (ALS) er en fatal neurodegenerativ lidelse resulterer i progressiv degeneration af motoneuroner. Peak debut er omkring 60 år for sporadisk sygdom og omkring 50 år for familiær sygdommen. På grund af sin progressivt forløb, 50% af patienterne dør inden for 30 måneder fra symptomdebut. For at evaluere hidtil ukendte behandlingsmuligheder for denne sygdom, har genetiske musemodeller for ALS er genereret ud fra human familiær mutationer i SOD-genet, såsom SOD1 (G93A) mutation. De fleste vigtige aspekter, der skal evalueres i modellen er samlet overlevelse, kliniske forløb og motorisk funktion. Her vil vi demonstrere den kliniske evaluering, viser ledning af to adfærdsmæssige motoriske tests og give kvantitative pointsystemer for alle parametre. Fordi en dybtgående analyse af ALS musemodel normalt kræver en immunhistokemisk undersøgelse af rygmarven, vi demonstrere sin forberedelse i detaljer anvende gøreRsaI laminektomi metode. Eksempler på histologiske fund er påvist. Den omfattende anvendelse af de afbildede eksamen metoder i undersøgelser af musemodel af ALS vil gøre det muligt for forskeren at pålideligt teste fremtidige terapeutiske muligheder, der kan danne grundlag for senere humane kliniske forsøg.

Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS

En musemodel for amyotrofisk lateral sklerose (ALS) undersøges klinisk og adfærdsmæssigt. Som en forudsætning for en ledsagende immunhistologisk analyse udarbejdelsen af ​​rygmarven er afbildet i detaljer.

Klinisk Test og fjernelse af rygmarv i en musemodel for Amyotrofisk Lateral Sclerose (ALS)

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder resulting in progressive degeneration of motoneurons. Peak of onset is around 60 ...

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Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis ALS

Improved methods for assessing bulbar impairment are necessary for expediting diagnosis of bulbar dysfunction in ALS, for predicting disease progression ...

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

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Akut og kronisk Tactile sensorisk analyse efter Rygmarvsskader i rotter

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

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The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis

Utility af dissocierede Intrinsic Hand muskelatrofi i diagnosen af ​​Amyotrofisk Lateral Sclerose

The split hand phenomenon refers to predominant wasting of thenar muscles and is an early and specific feature of amyotrophic lateral sclerosis (ALS). A ...

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Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

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High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Calibrated Forceps Model of Spinal Cord Compression Injury

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Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...

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A Neonatal Mouse Spinal Cord Compression Injury Model

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Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...