Name: clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)
Uploaded: 2012-03-17T14:00:00
Description: Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

LT ha ricevuto sovvenzioni da parte del Forschungsförderungsprogramm della Medicina dell&#39;Università di Göttingen. PL e MB sono stati sostenuti dal Centro di Ricerca per la DFG Fisiologia molecolare del cervello (CMPB), Göttingen. Gli autori ringraziano il Dott. Lars Tatenhorst per l&#39;assistenza e la videografia con Birgit Liebau aiuto con audio e video editing.

Gli animali sono stati acquistati da Jackson Laboratory (# 002.726) 1. Essi sono clinicamente segnato e sottoposto ad un test di funzionamento del motore (rotarod test) e della forza muscolare (appesa prova del filo). Tutti questi test e il successivo abbattimento degli animali al fine di preparare il midollo spinale sono stati eseguiti in accordo molto vicino alle linee guida locali per corretto svolgimento degli esperimenti sugli animali. 1. Punteggio clinico <p class="jove_co...

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Il (G93A) modello murino SOD1 genetico è un modello animale valido per studiare il corso della malattia del motoneurone perdita progressiva paragonabile umana sclerosi amiotrofica laterale 8. Una varietà di paradigmi di trattamenti diversi sono stati valutati in questo modello e rappresentano una base per la successiva prova in studi clinici umani 8-10. Per poter rilevare differenze significative in uno studio sperimentale di trattamento in questi topi, è di importanza eminente includere almeno ...

<table border="1"><tbody><tr><td></td><td> Fabbricante </td><td> Informazioni per l&#39;ordinazione </td></tr><tr><td> Test comportamentale </td><td></td><td></td></tr><tr><td> Rota-Rod per il Mice </td><td> Ugo Basile, Comerio, Italia </td><td> # 47600 </td></tr><tr><td> Hanging dispositivo a filo </td><td> Su misura </td><td></td></tr><tr><td colspan="3"></td></tr><tr><td> Preparazione Spinal Cord </td><td></td><td></td></tr><tr><td> Operation Table Spia di funzionamento Guanti di protezione </td><td></td><td></td></tr><tr><td> &quot;Iris&quot; Forbici, angolato a lato </td><td> Strumenti Scienza Belle, Heidelberg, Germania </td><td> 14063-09 </td></tr><tr><td> Cohan-Vännäs Forbici primavera, diritte </td><td> Strumenti Scienza Belle, Heidelberg, Germania </td><td> 15000-10 </td&gt; </tr><tr><td> Micro pinza </td><td> Hammacher, Solingen, Germania </td><td> HWC 111-10 </td></tr><tr><td> Scalpel &quot;präzisa plus&quot; </td><td> Dahlhausen, Köln, Germania </td><td> 11.000.00.510, figura 10 </td></tr></tbody></table>

clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)

La sclerosi laterale amiotrofica (SLA) è una malattia neurodegenerativa letale con conseguente progressiva degenerazione dei motoneuroni. Il picco di insorgenza è di circa 60 anni per la malattia sporadica e circa 50 anni per la malattia familiare. Grazie al suo andamento progressivo, il 50% dei pazienti muore entro 30 mesi dall&#39;inizio dei sintomi. Per valutare nuove opzioni di trattamento per questa malattia, modelli murini di SLA genetici sono stati generati sulla base umani mutazioni nel gene familiari SOD, come la (G93A) mutazione SOD1. Aspetti più importanti che devono essere valutati nel modello sono la sopravvivenza globale, decorso clinico e la funzione motoria. Qui, dimostriamo la valutazione clinica, mostrano la conduzione di due test del motore comportamentali e fornire sistemi di punteggio per tutti i parametri quantitativi. Poiché un&#39;analisi approfondita del modello murino ALS di solito richiede un esame immunoistochimica del midollo spinale, abbiamo dimostrato la sua preparazione in dettaglio l&#39;applicazione del farelaminectomia metodo rsal. Esemplari risultati istologici sono dimostrati. La completa applicazione dei metodi di esame descritti negli studi sul modello murino di SLA consentirà al ricercatore di verificare in modo affidabile per future opzioni terapeutiche che possono fornire una base per le sperimentazioni cliniche umane successive.

Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS

Un modello murino per la sclerosi laterale amiotrofica (SLA) è una valutazione clinica e comportamentale. Come prerequisito per un&#39;analisi accompagnamento immunoistologico la preparazione del midollo spinale è raffigurato in dettaglio.

Test clinici e la rimozione del midollo spinale in un modello murino di sclerosi laterale amiotrofica (SLA)

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder resulting in progressive degeneration of motoneurons. Peak of onset is around 60 ...

Research

JoVE Journal

Medicine

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis ALS

Un protocollo per la valutazione completa della disfunzione bulbare nella Sclerosi Laterale Amiotrofica (SLA)

Improved methods for assessing bulbar impairment are necessary for expediting diagnosis of bulbar dysfunction in ALS, for predicting disease progression ...

Ricerca

Medicina

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

Intraspinale trapianto di cellule cervicali targeting per corno ventrale nella Sclerosi Laterale Amiotrofica e le lesioni traumatiche del midollo spinale

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Acuta e Cronica test sensoriale tattile dopo lesione del midollo spinale in ratti

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusion Model of Severe Spinal Cord Injury in Rats

Un modello Contusione di gravi lesioni del midollo spinale nei ratti

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis

Utilità di dissociata intrinseca mano Atrofia muscolare nella diagnosi di Sclerosi Laterale Amiotrofica

The split hand phenomenon refers to predominant wasting of thenar muscles and is an early and specific feature of amyotrophic lateral sclerosis (ALS). A ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Controllata corticale modello Impact per trauma cranico

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

Un sistema radio-telemetria per monitorare la funzionalità cardiovascolare in ratti con midollo spinale e Transection embrionali staminali neurali Innesti cellulari

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Calibrato forcipe Modello di Spinal Cord Injury Compression

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

Un Sistema di punteggio rapida fenotipica neurologica per la valutazione di progressione della malattia nel SOD1-G93A modello murino della SLA

The SOD1-G93A transgenic mouse is the most widely used animal model of amyotrophic lateral sclerosis (ALS). At ALS TDI we developed a phenotypic screening ...

A Neonatal Mouse Spinal Cord Compression Injury Model

Un neonatale mouse Spinal Cord Injury compressione Modello

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...