Name: clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)
Uploaded: 2012-03-17T14:00:00
Description: Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

LT har mottatt stipend støtte fra Forschungsförderungsprogramm ved Universitetet Medicine Göttingen. PL og MB ble støttet av DFG Research Center for molekylær fysiologi av hjernen (CMPB), Göttingen. Forfatterne takker Dr. Lars Tatenhorst for hjelp med videography og Birgit Liebau for hjelp med lyd-og videoredigering.

Dyrene ble kjøpt fra Jackson Laboratory (# 002726) 1. De er klinisk scoret og utsatt for en test av motorisk funksjon (rotarod test) og muskulær styrke (hengende ledning test). Alle disse testene og senere avliving av dyrene for å forberede ryggmargen har blitt utført i tett henhold til de lokale retningslinjene for forsvarlig gjennomføring av dyreforsøk. 1. Klinisk Resultat Bortsett fra vurderingen for kroppsvekt mus blir undersøkt for tegn på m...

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Den SOD1 (G93A) genetisk mus modell er et verdifullt dyr modell for å studere sykdommen løpet av progressiv motoneuron tap sammenlignes med menneskelig amyotrofisk lateral sklerose 8. En rekke ulike behandlingstilbud paradigmer har blitt evaluert i denne modellen, og representerer et grunnlag for senere testing i kliniske studier 8-10. For å kunne oppdage signifikante forskjeller i en eksperimentell behandlingsstudie i disse musene, er det av største viktighet å omfatte minst 24 kull-matchet k...

<table border="1"><tbody><tr><td></td><td> Produsent </td><td> Bestillingsinformasjon </td></tr><tr><td> Behavioral testing </td><td></td><td></td></tr><tr><td> Rota-Rod for Mus </td><td> Ugo Basile, Comerio, Italia </td><td> # 47600 </td></tr><tr><td> Hengende ledning enhet </td><td> Custom-made </td><td></td></tr><tr><td colspan="3"></td></tr><tr><td> Spinal Cord Forberedelse </td><td></td><td></td></tr><tr><td> Drift Tabell Drift lampe Vernehansker </td><td></td><td></td></tr><tr><td> &quot;Iris&quot; Saks, vinklet til side </td><td> Fin Science Verktøy, Heidelberg, Tyskland </td><td> 14063-09 </td></tr><tr><td> Cohan-Vännäs Spring Saks, rette </td><td> Fin Science Verktøy, Heidelberg, Tyskland </td><td> 15000-10 </td&gt; </tr><tr><td> Micro tang </td><td> Hammacher, Solingen, Tyskland </td><td> HWC 111-10 </td></tr><tr><td> Skalpell &quot;präzisa pluss&quot; </td><td> Dahlhausen, Köln, Tyskland </td><td> 11.000.00.510, 10 FIG </td></tr></tbody></table>

clinical testing and spinal cord removal in a mouse model for amyotrophic lateral sclerosis (als)

Amyotrofisk lateral sklerose (ALS) er en fatal nevrodegenerativ lidelse som fører til progressiv degenerasjon av motoneurons. Peak av utbruddet er rundt 60 år for den sporadiske sykdommen og rundt 50 år for familiær sykdom. På grunn av sin progressive kurs, 50% av pasientene dør innen 30 måneder etter symptomdebut. For å kunne vurdere nye behandlingsalternativer for denne sykdommen, har genetiske musemodeller av ALS er generert basert på menneskelige familiære mutasjoner i SOD genet, som SOD1 (G93A) mutasjon. De fleste viktige aspekter som må vurderes i modellen er total overlevelse, klinisk forløp og motorisk funksjon. Her viser vi den kliniske vurderingen, viser gjennomføring av to atferdsmessige motoriske tester og gi kvantitative scoring systemer for alle parametere. Fordi en grundig analyse av ALS mus modell krever vanligvis en immunhistokjemisk undersøkelse av ryggmargen, viser vi til utarbeidelse i detalj bruk av dorsal laminektomi metode. Eksemplariske histologiske funn blir demonstrert. Den omfattende bruken av de avbildede undersøkelsesmetoder i studier på mus modell av ALS vil gjøre forskeren til pålitelig teste fremtidige terapeutiske muligheter som kan gi grunnlag for senere kliniske studier.

Watch this Scientific Journal Video about Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS at JoVE.com

Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis ALS

En mus modell for amyotrofisk lateral sklerose (ALS) er undersøkt klinisk og behaviorally. Som en forutsetning for en ledsagende immunhistokjemi analyse utarbeidelse av ryggmargen er avbildet i detalj.

Klinisk testing og Spinal Cord fjerning i en mus modell for Amyotrofisk lateral sklerose (ALS)

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder resulting in progressive degeneration of motoneurons. Peak of onset is around 60 ...

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Clinical Testing and Spinal Cord Removal in a Mouse Model for Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis (ALS)

A Protocol for Comprehensive Assessment of Bulbar Dysfunction in Amyotrophic Lateral Sclerosis ALS

Improved methods for assessing bulbar impairment are necessary for expediting diagnosis of bulbar dysfunction in ALS, for predicting disease progression ...

Intraspinal Cell Transplantation for Targeting Cervical Ventral Horn in Amyotrophic Lateral Sclerosis and Traumatic Spinal Cord Injury

Respiratory compromise due to phrenic motor neuron loss is a debilitating consequence of a large proportion of human traumatic spinal cord injury (SCI) ...

Acute and Chronic Tactile Sensory Testing after Spinal Cord Injury in Rats

Akutt og kronisk Taktil Sensory Testing etter ryggmargsskade i Rats

Spinal cord injury (SCI) impairs sensory systems causing allodynia1-8. To identify cellular and molecular causes of allodynia, sensitive and valid sensory ...

A Contusion Model of Severe Spinal Cord Injury in Rats

En Contusion modell av alvorlig ryggmargsskade i rotter

The translational potential of novel treatments should be investigated in severe spinal cord injury (SCI) contusion models. A detailed methodology is ...

Utility of Dissociated Intrinsic Hand Muscle Atrophy in the Diagnosis of Amyotrophic Lateral Sclerosis

Nytten av Spaltet Intrinsic Hånd muskelatrofi i diagnostikk av amyotrofisk lateral sklerose

The split hand phenomenon refers to predominant wasting of thenar muscles and is an early and specific feature of amyotrophic lateral sclerosis (ALS). A ...

Controlled Cortical Impact Model for Traumatic Brain Injury

Kontrollert Cortical Impact Modell for traumatisk hjerneskade

Every year over a million Americans suffer a traumatic brain injury (TBI).  Combined with the incidence of TBIs worldwide, the physical, emotional, ...

A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts

En Radio-telemetrisk system for å overvåke kardiovaskulær funksjon i rotter med ryggmargstran og Embryonic Neural Stem Cell Grafts

High thoracic or cervical spinal cord injury (SCI) can lead to cardiovascular dysfunction. To monitor cardiovascular parameters, we implanted a catheter ...

Calibrated Forceps Model of Spinal Cord Compression Injury

Calibrated pins Modell av ryggmargskompresjon Injury

Compression injuries of the murine spinal cord are valuable animal models for the study of spinal cord injury (SCI) and spinal regenerative therapy. The ...

A Quick Phenotypic Neurological Scoring System for Evaluating Disease Progression in the SOD1-G93A Mouse Model of ALS

En rask Fenotypisk Nevrologisk Scoring System for evaluering av sykdomsprogresjon i SOD1-G93A musemodell for ALS

The SOD1-G93A transgenic mouse is the most widely used animal model of amyotrophic lateral sclerosis (ALS). At ALS TDI we developed a phenotypic screening ...

A Neonatal Mouse Spinal Cord Compression Injury Model

En Neonatal Mouse ryggmargskompresjon Injury Model

Spinal cord injury (SCI) typically causes devastating neurological deficits, particularly through damage to fibers descending from the brain to the spinal ...