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DOI :
10.3791/3938-v
•
13:11 min
July 12th, 2012
Chapters
0:05
Title
1:58
Programming by Early Life Adversity
2:45
Collecting Brain Tissue Punches
4:02
Nucleic Acid Extraction from Brain Punches
6:08
Bisulfite PCR
7:41
Bisulfite Sequencing
10:57
The Effect of ELS on the Expression and Methylation of AVP
12:35
Conclusion
一个精简的工作流程,研究DNA甲基化和基因表达变化后早期生活压力。从新生鼠和离散脑组织隔离母体分离开始,我们代表的协议,同时为随后的亚硫酸氢钠测序和RT-PCR分析DNA和RNA分离脑组织拳。
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