Name: roux-en-y gastric bypass operation in rats
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Description: Watch this Scientific Journal Video about Roux-en-Y Gastric Bypass Operation in Rats at JoVE.com

马尔科Bueter和弗洛里安希耶福里德由德意志研究联合会（DFG）的支持。托马斯卢茨由瑞士国家研究基金会（SNF）的支持。马尔科Bueter和托马斯卢茨进一步收到来自美国国立卫生研究院（NIH）和从苏黎世中心（ZIHP）结合人体生理学的资金。卡雷尔W·勒鲁，由卫生部临床医师科学家奖部的支持。伦敦帝国学院收到从NIHR生物医学研究中心资助计划的支持。

1。术前护理<ol><li>从手术前大鼠过夜的食物。 </li><li>诱导麻醉室用4-5％的异氟醚和O2流量2升/分钟。 </li><li>从胸骨到骨盆使用电动剃须刀刮胡子腹部。 </li><li>放置在麻醉大鼠仰卧位温加热垫。 </li><li>申请之前放置在老鼠的头锥吻眼膏（Vitagel）。 </li><li>麻醉维持2-3％的异氟醚浓度和氧流量2升/分钟。 </li><li>解决方案与优碘消毒皮肤。 </li><li>确认麻醉深度，用钳子捏脚趾之间的后腿。 </li><li>围?...

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D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exogenous+peptide+YY3-36+and+Exendin-4+further+decrease+food+intake,+whereas+octreotide+increases+food+intake+in+rats+after+Roux-en-Y+gastric+bypass.">Exogenous peptide YY3-36 and Exendin-4 further decrease food intake, whereas octreotide increases food intake in rats after Roux-en-Y gastric bypass.</a> Int. J. Obes. (Lond). , (2011).</li><li>Kreymann, B., Williams, G., Ghatei, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucagon-like+peptide-1+7-36:+a+physiological+incretin+in+man.">Glucagon-like peptide-1 7-36: a physiological incretin in man.</a> Lancet. 2, 1300-1304 (1987).</li><li>le Roux, C. W., Batterham, R. L., Aylwin, S. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Techniques+of+laparoscopic+gastric+bypass:+on-line+survey+of+American+Society+for+Bariatric+Surgery+practicing+surgeons.">Techniques of laparoscopic gastric bypass: on-line survey of American Society for Bariatric Surgery practicing surgeons.</a> Surg. Obes. Relat. Dis. 4, 166-172 (2008).</li><li>Bueter, M., Lowenstein, C., Ashrafian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vagal+sparing+surgical+technique+but+not+stoma+size+affects+body+weight+loss+in+rodent+model+of+gastric+bypass.">Vagal sparing surgical technique but not stoma size affects body weight loss in rodent model of gastric bypass.</a> Obes. Surg. 20, 616-622 (2010).</li><li>Korner, J., Bessler, M., Cirilo, L. 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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+inhibitory+effects+of+peripheral+administration+of+peptide+YY(3-36)+and+glucagon-like+peptide-1+on+food+intake+are+attenuated+by+ablation+of+the+vagal-brainstem-hypothalamic+pathway.">The inhibitory effects of peripheral administration of peptide YY(3-36) and glucagon-like peptide-1 on food intake are attenuated by ablation of the vagal-brainstem-hypothalamic pathway.</a> Brain Res. 1044, 127-131 (2005).</li><li>Adrian, T. E., Ballantyne, G. H., Longo, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Deoxycholate+is+an+important+releaser+of+peptide+YY+and+enteroglucagon+from+the+human+colon.">Deoxycholate is an important releaser of peptide YY and enteroglucagon from the human colon.</a> Gut. 34, 1219-1224 (1993).</li><li>Nakatani, H., Kasama, K., Oshiro, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serum+bile+acid+along+with+plasma+incretins+and+serum+high-molecular+weight+adiponectin+levels+are+increased+after+bariatric+surgery.">Serum bile acid along with plasma incretins and serum high-molecular weight adiponectin levels are increased after bariatric surgery.</a> Metabolism. 58, 1400-1407 (2009).</li><li>Ashrafian, H., le Roux, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+surgery+and+gut+hormones+-+a+review+of+bariatric+entero-humoral+modulation.">Metabolic surgery and gut hormones - a review of bariatric entero-humoral modulation.</a> Physiol. Behav. 97, 620-631 (2009).</li><li>Choban, P. S., Flancbaum, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+Roux+limb+lengths+on+outcome+after+Roux-en-Y+gastric+bypass:+a+prospective,+randomized+clinical+trial.+Obes.">The effect of Roux limb lengths on outcome after Roux-en-Y gastric bypass: a prospective, randomized clinical trial. Obes.</a> Surg. 12, 540-545 (2002).</li><li>Gleysteen, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Five-year+outcome+with+gastric+bypass:+Roux+limb+length+makes+a+difference.">Five-year outcome with gastric bypass: Roux limb length makes a difference.</a> Surg. Obes. Relat. Dis. 5, 242-247 (2009).</li><li>Lee, S., Sahagian, K. G., Schriver, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+between+varying+Roux+limb+lengths+and+weight+loss+in+gastric+bypass.">Relationship between varying Roux limb lengths and weight loss in gastric bypass.</a> Curr. Surg. 63, 259-263 (2006).</li><li>Hayes, M. R., Kanoski, S. E., De Jonghe, B. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+common+hepatic+branch+of+the+vagus+is+not+required+to+mediate+the+glycemic+and+food+intake+suppressive+effects+of+glucagon-like-peptide-1.">The common hepatic branch of the vagus is not required to mediate the glycemic and food intake suppressive effects of glucagon-like-peptide-1.</a> Am. J. Physiol. Regul. Integr. Comp. Physiol. 301, R1479-R1485 (2011).</li></ol>

在人类空肠Roux-en-Y胃旁路手术的过程是由梅森在1967年首次描述，并修改其目前的形式由托雷斯在1983年19。目前，该程序包括胃小囊和近端小肠旁路。 图1给出了一个在术前和术后的解剖示意图。 在人类的胃绕道手术诱发和保持身体约15-30％的重量损失。期间减少食物的摄入量，改变食物的喜好和大概增加能源开支4-6,8-10部分原因是由于手术后的头?...

<table border="1"><tbody><tr><td> 通用名称 </td><td> 品牌名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>恩诺沙星</td><td> baytril 2.5％ </td><td> provet公司</td><td> 1036 </td></tr><tr><td>氟尼辛</td><td> finadyne </td><td> graeub </td><td> 908040 </td></tr><tr><td> buprenorphin </td><td> temgesic </td><td>利洁时</td><td> 138976 </td></tr><tr><td>异氟醚</td><td> IsoFlo </td><td> graeub </td><td> 902035 </td></tr><tr><td>维生素A </td><td> vitagel </td><td>博士伦</td><td> 690 </td></tr><tr><td>碘溶液</td><td>优碘</td><td> mundipharma </td><td> 111141 </td></tr><tr><td>氯化钠0.9％ </td><td>氯化钠0.9％ </td><td>贝朗</td><td> 534534 </td></tr></tbody></table> 表1。药品。 <table border="1"><tbody><tr><td> 名称 </td><td> 大小 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td> PDS的二</td><td> 4-0 </td><td>爱惜康</td><td> Z924H </td></tr><tr><td> PDS的二</td><td> 5-0 </td><td>爱惜康</td><td> Z925H </td></tr><tr><td> PDS的二</td><td> 6-0 </td><td>爱惜康</td><td> PUU2971E </td></tr><tr><td> PDS的二</td><td> 7-0 </td><td>爱惜康</td><td> Z1370E </td></tr><tr><td> vicryl </td><td> 4-0 </td><td>爱惜康</td><td> V451H </td></tr></tbody></table> 表2。缝线。 <table border="1"><tbody><tr><td> 名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>手术刀手柄第3号</td><td>蛇牌</td><td> BB073R </td></tr><tr><td>没有手术刀片。 10 </td><td>斯旺 - 莫顿</td><td> 0301 </td></tr><tr><td>持针器</td><td>蛇牌</td><td> BM124R </td></tr><tr><td>组织钳</td><td>蛇牌</td><td> BD555R </td></tr><tr><td>梅岑鲍姆剪刀，直</td><td>蛇牌</td><td> BC022R </td></tr><tr><td>梅岑鲍姆剪刀，弯曲</td><td>蛇牌</td><td> BC023R </td></tr><tr><td>精致的剪刀，弯曲</td><td>蛇牌</td><td> BC061R </td></tr><tr><td>动脉钳，弯曲</td><td>蛇牌</td><td> BH109R </td></tr><tr><td>动脉钳，弯曲，1X2的牙齿</td><td>蛇牌</td><td> BH121R </td></tr><tr><td>探头，双端</td><td>蛇牌</td><td> BN113R </td></tr><tr><td>微针座</td><td>蛇牌</td><td>调频541R </td></tr><tr><td>微型镊子</td><td>蛇牌</td><td> FM571R </td></tr><tr><td>微型剪刀</td><td> AESCulap </td><td> FM470R </td></tr><tr><td>一次性眼烧灼</td><td>约翰·维斯国际</td><td> 0111122 </td></tr><tr><td>棉花棒</td><td>哈特曼公司</td><td> 9679369 </td></tr></tbody></table> 表3。外科手术设备。

roux-en-y gastric bypass operation in rats

目前，最有效的疗法是治疗病态肥胖引起重大和维持身体消瘦，一个成熟的死亡率受益减肥手术1,2。因此，出现了近年来空肠Roux-en-Y胃旁路（胃绕道）是最常执行的操作3全球减肥行动的数量在稳步上升。在此背景下，重要的是要了解胃绕道诱导和保持身体消瘦的生理机制。这些机制还没有完全理解，但可能包括减少饥饿和增加的饱食4,5，能源开支增加6,7，改变食物中的脂肪和糖8,9高，改变盐和水的处理，肾脏10偏好以及在肠道菌群的改变11。胃绕道手术后见过这样的变化可能至少部分源于如何手术改变荷尔蒙的环境，因为胃绕道手术会增加餐后释放肽YY（PYY）和胰高血糖素样肽1（GLP-1），营养物质的存在是由肠道释放激素，减少吃12。 在过去二十年中已经开展了许多研究使用大鼠胃绕道手术后的生理变化，以进一步调查。胃绕道大鼠模型已被证明是一个有价值的实验工具并非最不重要的，因为它与模拟时间剖面和人类减肥的幅度，但也使研究人员能够控制和操纵关键的解剖和生理因素，包括使用适当的控制。因此，有各种各样的大鼠胃旁路模式在审查更多的细节13日至15日在其他地方的文学。这些模型的精确的手术技术的描述差别很大，并在袋大小不同，例如，肢体长度和迷走神经的保护。如果报道，死亡率似乎范围从0到35％，15。此外，手术已经进行了几乎完全不同品系和年龄的雄性大鼠。术前和术后的饮食也差异显着。 在公布的胃绕道手术大鼠模型和实验技术的变化复杂的胃绕道手术所涉及的潜在生理机制的比较和鉴别。有没有明确的证据，这些模型是优越的，但有一个标准化的程序的新兴需要取得一致和可比的数据。因此，本文旨在总结和讨论我们以前审定并公布的胃绕道手术大鼠模型的技术和实验的细节。

Watch this Scientific Journal Video about Roux-en-Y Gastric Bypass Operation in Rats at JoVE.com

Roux-en-Y Gastric Bypass Operation in Rats

最近大量使用胃绕道大鼠模型的研究已进行了揭露空肠Roux-en-Y胃绕道手术的基本生理机制。本文旨在展示和讨论我们公布的胃绕道手术大鼠模型的技术和实验的细节，以了解本实验工具的优势和局限性。

空肠Roux-en-Y胃搭桥手术大鼠

Currently, the most effective therapy for the treatment of morbid obesity to induce significant and maintained body weight loss with a proven mortality ...

Research

JoVE Journal

Medicine

Acute Myocardial Infarction in Rats

大鼠急性心肌梗死

With heart failure leading the cause of death in the USA (Hunt), biomedical research is fundamental to advance medical treatments for cardiovascular ...

科研

医学

Orthotopic Liver Transplantation in Rats

大鼠原位肝移植

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Intraoperative Gastroscopy for Tumor Localization in Laparoscopic Surgery for Gastric Adenocarcinoma

胃镜检查术在腹腔镜手术的肿瘤定位于胃腺癌

Determining resection margins for gastric cancer, which are not exposed to the serosal surface of the stomach, is the most important procedure during ...

Vein Interposition Model: A Suitable Model to Study Bypass Graft Patency

静脉介入型号：研究旁路移植通畅一个合适的模型

Bypass grafting is an established treatment method for coronary artery disease. Graft patency continues to be the Achilles heel of saphenous vein grafts. ...

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

袖胃切除术的技术和改良的小鼠空肠Roux-en-Y胃旁路

Obesity is a major public health issue, with a prevalence of 4 to 28% for men and 6.2 to 36.5% for women in Europe (from 2003 to 2008). Morbid obesity is ...

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

小鼠体外循环模型: 一种新方法

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...

One-anastomosis Gastric Bypass (OAGB) in Rats

One-anastomosis Gastric Bypass OAGB in Rats

大鼠胃旁路 (oagb) 的一次吻合

The goal of this protocol is to set up a preclinical model of bariatric surgery and, more specifically, OAGB in obese rats. Based on this preclinical ...

Preparation of Decellularized Kidney Scaffolds in Rats

在大鼠中准备去细胞化肾脏脚手架

Tissue engineering is a cutting-edge discipline in biomedicine. Cell culture techniques can be applied for regeneration of functional tissues and organs ...

A Protocol for Roux-en-Y Gastric Bypass in Rats using Linear Staplers

使用线性压花器在老鼠身上进行胃旁路的规程

Roux-en-Y gastric bypass (RYGB) is commonly performed for the treatment of severe obesity and type 2 diabetes. However, the mechanism of weight loss and ...

Robotic Central Pancreatectomy with Roux-en-Y Pancreaticojejunostomy

机器人中央胰腺切除术与鲁恩-Y胰腺切除术

Central pancreatectomy is a parenchyma-sparing alternative to distal pancreatectomy in patients with a benign or low-grade malignant tumor in the body of ...