Name: roux-en-y gastric bypass operation in rats
Uploaded: 2012-06-11T13:00:00
Description: Watch this Scientific Journal Video about Roux-en-Y Gastric Bypass Operation in Rats at JoVE.com

Marco Bueter ve Florian Seyfried Deutsche Forschungsgemeinschaft (DFG) tarafından desteklendi. Thomas A Lutz İsviçre Ulusal Araştırma Vakfı (SNF) tarafından desteklenmiştir. Marco Bueter ve Thomas bir başka Ulusal Sağlık Enstitüsü (NIH) gelen ve Bütünleştirici İnsan Fizyolojisi için Zürih Merkezi (ZIHP) desteğinden Lutz. Carel W le Roux Sağlık Klinisyen bir bilim adamı ödülü Bölümü tarafından desteklenmiştir. Imperial College London NIHR Biyomedikal Araştırma Merkezi fon programından destek almaktadır.

1. Preoperatif Bakım <ol><li> Ameliyattan bir gece önce sıçan gıda çıkarın. </li><li> 2 lt / dk% 4-5 izofluran ve O2 akışı ile odasında anestezi neden. </li><li> Sternumdan elektrikli tıraş makinesi kullanılarak pelvise karın Tıraş. </li><li> Izotermal ısıtma yastığı sırtüstü pozisyonda anestezi sıçan yerleştirin. </li><li> Roket ucu konisi içinde farelerin burun yerleştirmeden önce göz merhemi (Vitagel) uygulayın. </li><li> % 2-3 izofluran konsantrasyonu ve 2 lt / dak O2 akışı ile...

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İnsanlarda Roux-en-Y gastrik bypass prosedürü 1967 yılında Mason tarafından açıklanan ve 1983 19 Torres&#39;in bugünkü formuna güncellenmiştir. Şu anda, prosedürü küçük bir gastrik poş ve proksimal ince barsak bypass oluşur. Ameliyat öncesi ve sonrası anatomisinin şematik bir resmidir Şekil 1 &#39;de verilmiştir. İnsanlarda gastrik bypass yaklaşık% 15-30 2 kilo kaybına neden olur ve korur. Vücut ağırlığının çoğunlu?...

<table border="1"><tbody><tr><td> Jenerik adı </td><td> Marka adı </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> Enrofloxacin </td><td> Baytril% 2.5 </td><td> Provet AG </td><td> 1036 </td></tr><tr><td> Flunixin </td><td> Finadyne </td><td> Graeub </td><td> 908040 </td></tr><tr><td> Buprenorphin </td><td> Temgesic </td><td> Reckitt Benckiser </td><td> 138976 </td></tr><tr><td> Izofluran </td><td> IsoFlo </td><td> Graeub </td><td> 902035 </td></tr><tr><td> A vitamini </td><td> Vitagel </td><td> Bausch &amp; Lomb </td><td> 690 </td></tr><tr><td> Iyot çözeltisi </td><td> Betadine </td><td> Mundipharma </td><td> 111141 </td></tr><tr><td> % 0.9 NaCl </td><td> % 0.9 NaCl </td><td> B. Braun </td><td> 534534 </td></tr></tbody></table> Tablo 1. Ilaçlar. <table border="1"><tbody><tr><td> Isim </td><td> Boyut </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> PDS II </td><td> 4-0 </td><td> Ethicon </td><td> Z924H </td></tr><tr><td> PDS II </td><td> 5-0 </td><td> Ethicon </td><td> Z925H </td></tr><tr><td> PDS II </td><td> 6-0 </td><td> Ethicon </td><td> PUU2971E </td></tr><tr><td> PDS II </td><td> 7-0 </td><td> Ethicon </td><td> Z1370E </td></tr><tr><td> Vicryl </td><td> 4-0 </td><td> Ethicon </td><td> V451H </td></tr></tbody></table> Tablo 2. Dikişler. <table border="1"><tbody><tr><td> Isim </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> Bisturi sapı No: 3 </td><td> Aesculap </td><td> BB073R </td></tr><tr><td> Bisturi bıçakları yok. 10 </td><td> Swann-Morton </td><td> 0301 </td></tr><tr><td> İğne tutucu </td><td> Aesculap </td><td> BM124R </td></tr><tr><td> Doku forseps </td><td> Aesculap </td><td> BD555R </td></tr><tr><td> Metzenbaum makas, düz </td><td> Aesculap </td><td> BC022R </td></tr><tr><td> Metzenbaum makas, kavisli </td><td> Aesculap </td><td> BC023R </td></tr><tr><td> Hassas kavisli makas, </td><td> Aesculap </td><td> BC061R </td></tr><tr><td> Arter forseps, kavisli </td><td> Aesculap </td><td> BH109R </td></tr><tr><td> Arter forseps, kavisli, 1x2 dişler </td><td> Aesculap </td><td> BH121R </td></tr><tr><td> Prob, çift-uçlu </td><td> Aesculap </td><td> BN113R </td></tr><tr><td> Mikro iğne tutucu </td><td> Aesculap </td><td> FM 541R </td></tr><tr><td> Mikro forseps </td><td> Aesculap </td><td> FM571R </td></tr><tr><td> Mikro makas </td><td> AescULAP&#39;ın </td><td> FM470R </td></tr><tr><td> Tek göz koter </td><td> John Weiss Uluslararası </td><td> 0111122 </td></tr><tr><td> Pamuk tomurcukları </td><td> Hartmann AG </td><td> 9679369 </td></tr></tbody></table> Tablo 3. Cerrahi ekipmanlar.

roux-en-y gastric bypass operation in rats

Şu anda, kanıtlanmış bir ölüm parası ile önemli ve bakımı vücut ağırlığı kaybı sağlamaya yönelik morbid obezite tedavisi için en etkili tedavi obezite cerrahisi 1,2. Sonuç olarak, en sık yapılan operasyon 3 olan Roux-en-Y gastrik bypass (gastrik bypass) ile son yıllarda dünya çapında yapılan obezite operasyon sayısında sürekli bir artış olmuştur. Bu arka plana karşı, gastrik bypass indükler ve vücut ağırlığı kaybı sürekliliği açısından fizyolojik mekanizmaları anlamak önemlidir. Bu mekanizmalar henüz tam olarak anlaşılamamıştır, ancak düşük açlık içerebilir ve yağ ve şeker 8,9 yüksek gıda değiştirilmiş, tuz ve böbrek 10 su işleme olarak değiştirilmiş tercihi, doyma 4,5, artan enerji harcaması 6,7 arttı iyi gut Mikrobiyota 11 değişiklikler gibi. Gastrik bypass sonrası görülen tür değişiklikler en azından kısmen nasıl kaynaklanıyor olabilirGastrik bypass peptid-YY (PYY) ve glukagon benzeri peptit-1-(GLP-1) ile tokluk yayın, besinlerin varlığında gut tarafından yayımlanan hormonlar artar ve bu yeme azaltmak için cerrahi hormonal değiştirir 12. Son yirmi yılda sıçan kullanarak çok sayıda çalışmalar fazla gastrik bypass sonrası fizyolojik değişiklikleri araştırmak amacıyla yapılmıştır. Gastrik bypass sıçan modelinde yakından zaman profili ve insan kilo kaybı büyüklüğü taklit eden değil, aynı zamanda araştırmacılar uygun kontrollerin kullanılması gibi kritik anatomik ve fizyolojik faktörler kontrol ve manipüle etmeyi sağlayan olarak en değerli bir deney aracı değil olduğu kanıtlanmıştır. Sonuç olarak, başka bir yerde daha ayrıntılı 13-15 gözden literatürde mevcut sıçan gastrik bypass modelleri geniş bir dizi var. Bu modellerin kesin cerrahi tekniğin açıklaması büyük ölçüde değişmektedir ve kese büyüklüğü açısından örneğin farklıdır, uzuvuzunlukları ve vagal sinir korunması. Rapor ise, ölüm oranları 0 olarak% 35 15 arasında değişmektedir görünüyor. Ayrıca, cerrahi farklı suşları ve yaştaki erkek farelerde neredeyse sadece yapılmıştır. Ameliyat öncesi ve sonrası beslenme de önemli ölçüde değişmiştir. Yayınlanan gastrik bypass sıçan modellerinde Teknik ve deneysel varyasyonlar gastrik bypass dahil potansiyel fizyolojik mekanizmalar karşılaştırılması ve kimlik zorlaştırıyor. Bu modellerin herhangi bir üstün olduğunu net bir kanıt yoktur, ancak tutarlı ve karşılaştırılabilir veriler elde etmek prosedürünün standardizasyonu için yükselen bir ihtiyaç vardır. Dolayısıyla bu madde, daha önceden onaylanmış ve yayınlanmış gastrik bypass sıçan modelinin teknik ve deneysel ayrıntıları özetlemek ve tartışmayı amaçlamaktadır.

Watch this Scientific Journal Video about Roux-en-Y Gastric Bypass Operation in Rats at JoVE.com

Roux-en-Y Gastric Bypass Operation in Rats

Gastrik bypass sıçan modellerini kullanarak çok sayıda çalışma son zamanlarda Roux-en-Y gastrik bypass işlemleri altta yatan fizyolojik mekanizmaları ortaya çıkarmak için yapılmıştır. Bu makale, göstermek ve bu deneysel aracın avantajları ve sınırlamaları anlamak için bizim yayınlanan gastrik bypass sıçan modelinin teknik ve deneysel ayrıntıları tartışmayı amaçlamaktadır.

Sıçanlarda Roux-en-Y Gastrik Bypass Operasyonu

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