Name: detection of bacteria using fluorogenic dnazymes
Uploaded: 2012-05-28T13:00:00
Duration: 13 min 20 s
Description: Watch this Scientific Journal Video about Detection of Bacteria Using Fluorogenic DNAzymes at JoVE.com

מימון עבור עבודה זו ניתן על ידי למדעי הטבע וההנדסה המועצה למחקר בקנדה (NSERC) וברשת ביו סנטינל נייר.

<ol>
	<li>Zourob, M., Elwary, S., Truner, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems. , (2008).</li><li>Call, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Challenges+and+Opportunities+for+Pathogen+Detection+Using+DNA+Microarrays.">Challenges and Opportunities for Pathogen Detection Using DNA Microarrays.</a> Crit. Rev. Microbiol. 31, 91-99 (2005).</li><li>Lazcka, O., Campo, D. e. l., J, F., Muñoz, F. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogen+detection:+A+perspective+of+traditional+methods+and+biosensors.">Pathogen detection: A perspective of traditional methods and biosensors.</a> Biosens. Bioelectron. 22, 1205-1217 (2007).</li><li>Velusamy, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+overview+of+foodborne+pathogen+detection:+In+the+perspective+of+biosensors.">An overview of foodborne pathogen detection: In the perspective of biosensors.</a> Biotechnol. Adv. 28, 232-254 (2010).</li><li>Ali, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluorogenic+DNAzyme+Probes+as+Bacterial+Indicators.">Fluorogenic DNAzyme Probes as Bacterial Indicators.</a> Angew. Chem. Int. Ed. 50, 3751-3754 (2011).</li><li>Navani, N. K., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nucleic+acid+aptamers+and+enzymes+as+sensors.">Nucleic acid aptamers and enzymes as sensors.</a> Curr. Opin. Chem. Biol. 10, 272-281 (2006).</li><li>Liu, J., Cao, Z., Lu, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+Nucleic+Acid+Sensors.">Functional Nucleic Acid Sensors.</a> Chem. Rev. 109, 1948-1998 (2009).</li><li>Li, Y., Lu, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Functional Nucleic Acids for Analytical Applications. , (2009).</li><li>Tuerk, C., Gold, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+evolution+of+ligands+by+exponential+enrichment:+RNA+ligands+to+bacteriophage+T4+DNA+polymerase.">Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.</a> Science. 249, 505-510 (1990).</li><li>Ellington, A. D., Szostak, J. 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K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DNA+as+a+versatile+chemical+component+for+catalysis,+encoding,+and+stereocontrol.">DNA as a versatile chemical component for catalysis, encoding, and stereocontrol.</a> Angew. Chem. Int. Ed. 49, 7180-7201 (2010).</li><li>Mei, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+efficient+RNA-cleaving+DNA+enzyme+that+synchronizes+catalysis+with+fluorescence+signaling.">An efficient RNA-cleaving DNA enzyme that synchronizes catalysis with fluorescence signaling.</a> J. Am. Chem. Soc. 125, 412-420 (2003).</li><li>Liu, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assemblage+of+signaling+DNA+enzymes+with+intriguing+metal-ion+specificities+and+pH+dependences.">Assemblage of signaling DNA enzymes with intriguing metal-ion specificities and pH dependences.</a> J. Am. Chem. Soc. 125, 7539-7545 (2003).</li><li>Kandadai, S. A., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+a+catalytically+efficient+acidic+RNA-cleaving+deoxyribozyme.">Characterization of a catalytically efficient acidic RNA-cleaving deoxyribozyme.</a> Nucleic Acids Res. 33, 7164-7175 (2005).</li><li>Rupcich, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quenching+of+fluorophore-labeled+DNA+oligonucleotides+by+divalent+metal+ions:+implications+for+selection,+design,+and+applications+of+signaling+aptamers+and+signaling+deoxyribozymes.">Quenching of fluorophore-labeled DNA oligonucleotides by divalent metal ions: implications for selection, design, and applications of signaling aptamers and signaling deoxyribozymes.</a> J. Am. Chem. Soc. 128, 780-790 (2005).</li><li>Shen, Y., Brennan, J. D., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterizing+the+secondary+structure+and+identifying+functionally+essential+nucleotides+of+pH6DZ1,+a+fluorescence-signaling+and+RNA-cleaving+deoxyribozyme.">Characterizing the secondary structure and identifying functionally essential nucleotides of pH6DZ1, a fluorescence-signaling and RNA-cleaving deoxyribozyme.</a> Biochemistry. 44, 12066-12076 (2005).</li><li>Chiuman, W., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Revitalization+of+six+abandoned+catalytic+DNA+species+reveals+a+common+three-way+junction+framework+and+diverse+catalytic+cores.">Revitalization of six abandoned catalytic DNA species reveals a common three-way junction framework and diverse catalytic cores.</a> J. Mol. Biol. 357, 748-754 (2006).</li><li>Chiuman, W., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolution+of+high-branching+deoxyribozymes+from+a+catalytic+DNA+with+a+three-way+junction.">Evolution of high-branching deoxyribozymes from a catalytic DNA with a three-way junction.</a> Chem. Biol. 13, 1061-1069 (2006).</li><li>Shen, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Catalysis+and+rational+engineering+of+trans-acting+pH6DZ1,+an+RNA-cleaving+and+fluorescence-signaling+deoxyribozyme+with+a+four-way+junction+structure.">Catalysis and rational engineering of trans-acting pH6DZ1, an RNA-cleaving and fluorescence-signaling deoxyribozyme with a four-way junction structure.</a> Chem BioChem. 7, 1343-1348 (2006).</li><li>Ali, M. M., Kandadai, S. A., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+pH3DZ1+-+An+RNA-cleaving+deoxyribozyme+with+optimal+activity+at+pH+3.">Characterization of pH3DZ1 - An RNA-cleaving deoxyribozyme with optimal activity at pH 3.</a> Can. J. Chem. 85, 261-273 (2007).</li><li>Chiuman, W., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+signaling+platforms+built+from+a+small+catalytic+DNA+and+doubly+labeled+fluorogenic+substrates.">Efficient signaling platforms built from a small catalytic DNA and doubly labeled fluorogenic substrates.</a> Nucleic Acids Res. 35, 401-405 (2007).</li><li>Chiuman, W., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simple+fluorescent+sensors+engineered+with+catalytic+DNA+MgZ+based+on+a+non-classic+allosteric+design.">Simple fluorescent sensors engineered with catalytic DNA MgZ based on a non-classic allosteric design.</a> PLoS ONE. 2, e1224 (2007).</li><li>Shen, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Entrapment+of+fluorescence+signaling+DNA+enzymes+in+sol+gel-derived+materials+for+metal+ion+sensing.">Entrapment of fluorescence signaling DNA enzymes in sol gel-derived materials for metal ion sensing.</a> Anal. Chem. 79, 3494-3503 (2007).</li><li>Kandadai, S. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+an+RNA-cleaving+deoxyribozyme+with+optimal+activity+at+pH+5.">Characterization of an RNA-cleaving deoxyribozyme with optimal activity at pH 5.</a> Biochemistry. 48, 7383-7391 (2009).</li><li>Zhao, W., Brook, M. A., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Periodic+assembly+of+nanospecies+on+repetitive+DNA+sequences+generated+on+gold+nanoparticles+by+rolling+circle+amplification.">Periodic assembly of nanospecies on repetitive DNA sequences generated on gold nanoparticles by rolling circle amplification.</a> Methods Mol. Biol. 474, 79-90 (2008).</li><li>Navani, N. K., Mok, W. K., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+selection+of+protein-binding+DNA+aptamers+as+ligands+for+biosensing+applications.">In vitro selection of protein-binding DNA aptamers as ligands for biosensing applications.</a> Methods Mol. Biol. 504, 399-415 (2009).</li><li>Ali, M. M., Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colorimetric+sensing+by+using+allosteric-DNAzyme-coupled+rolling+circle+amplification+and+a+peptide+nucleic+acid-organic+dye+probe.">Colorimetric sensing by using allosteric-DNAzyme-coupled rolling circle amplification and a peptide nucleic acid-organic dye probe.</a> Angew. Chem. Int. Ed. 48, 3512-3515 (2009).</li></ol>

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רוב השיטות המקובלות לזיהוי חיידקים היום הם איטיים או (חיידקים קלאסי) או טכנית בדרישה (נוגדנים, PCR). לכן, אנו מאמינים כי הדור הבא של כלי איתור צריך לספק מהירות כלפי ופשטות. לצורך כך, יצרנו RNA-השחיטה ואת הקרינה, איתות DNAzyme שיכולים לשמש לפיתוח מבחני פשוטים לדווח נוכחות של חיידקים דרך הדור האות פלואורסצנטי. התכונות DNAzyme בדיקה, RFD-EC1, מופעל על ידי CEM הנוצר במהלך הצמיחה של א &#39; coli בתקשורת ובתרבות. מאז השיטה שלנו משתמשת בתערובות תאיים הגולמי של חיידק כיעד של איתור עוקף את מיצוי היעד מייגע וצעדים הגברה, ניתן להשתמש בו כדי להגדיר פשוט מאוד, &quot;תמהיל-and-לקרוא&quot; סוג של מבחני לגילוי חיידקי. השימוש DNAzyme שלנו אינו מוגבל בשיטה המבוססת על זיהוי הקרינה. לדוגמה, זיהוי colorimetric באמצעות אותה מערכת DNAzyme assay גלהיות מתוכנן בשיטה דיווחו בעבר כי מנצל הגברה מעגל מתגלגל יחד עם צבע אורגני. 32 אנו צופים את השימוש DNAzymes לגילוי חיידקי כמו השדרה אטרקטיבי ליצור biosensors חיידקים חדשים עם פשטות תפעולית גדולה יותר.

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> שם מגיב </td><td> חברה </td><td> מספר קטלוגי או מודל </td></tr><tr><td> אגר </td><td> BioShop קנדה </td><td> AGR003 </td></tr><tr><td> אמוניום persulfate (APS) </td><td> BioShop קנדה </td><td> AMP001 </td></tr><tr><td> Acrylamide / Bis-acrylamide (40%, 29:1) </td><td> BioShop קנדה </td><td> ACR004 </td></tr><tr><td> בור חומצה </td><td> BioShop קנדה </td><td> BOR001 </td></tr><tr><td> Bromophenol כחול </td><td> Sigma-Aldrich </td><td> B8026 </td></tr><tr><td> EDTA </td><td> א.מ. מדע </td><td> EXO539-1 </td></tr><tr><td> HCl </td><td> Sigma-Aldrich </td><td> 38281 </td></tr><tr><td> HEPES </td><td> Bioshop קנדה </td><td> HEP001 </td></tr><tr><td> LB מרק </td><td> Sigma-Aldrich </td><td> L3022 </td></tr><tr><td> MgCl 2 </td><td> EMDכימיקלים </td><td> B10149-34 </td></tr><tr><td> NaCl </td><td> BioShop קנדה </td><td> SOD002 </td></tr><tr><td> NaOAc </td><td> EMD כימיקלים </td><td> SXO255-1 </td></tr><tr><td> NaOH </td><td> EMD כימיקלים </td><td> SXO590-1 </td></tr><tr><td> SDS </td><td> BioShop קנדה </td><td> SDS001 </td></tr><tr><td> TEMED </td><td> BioShop קנדה </td><td> TEM001 </td></tr><tr><td> טריס בסיס </td><td> BioShop קנדה </td><td> BST666 </td></tr><tr><td> Tween 20 </td><td> Sigma-Aldrich </td><td> P9416 </td></tr><tr><td> אוריאה </td><td> BioShop קנדה </td><td> URE001 </td></tr><tr><td> Xylenecyanol FF </td><td> Sigma-Aldrich </td><td> X4126 </td></tr><tr><td> ה-DNA concentrator </td><td> Thermo Scientific </td><td> Savant DNA SpeedVac 120 </td></tr><tr><td> Millex יחידת המסנן </td><td> Millipore </td><td> SLGP033RS </td></tr><tr><td> ג&#39;ל טיפים העמסה </tד&gt; <td> Diamed </td><td> TEC200EX-K </td></tr><tr><td> ImageQuant תוכנה </td><td> מולקולרית דינמיקה </td><td> גרסה 5.0 </td></tr><tr><td> Kimwipes </td><td> קימברלי קלארק מקצועי </td><td> 34705 </td></tr><tr><td> מיני Vortexer </td><td> VWR </td><td> 58816-121 </td></tr><tr><td> Parafilm </td><td> Pechiney אריזות פלסטיק </td><td> PM996 </td></tr><tr><td> פטרי מנות </td><td> פישר סיינטיפיק </td><td> Fisherbrand 08-757-12 </td></tr><tr><td> Stripettor פלוס (פיפטה האקדח) </td><td> קורנינג </td><td> 07764714 </td></tr><tr><td> קוורץ cuvettes </td><td> וריאן Inc </td><td> 66-100216-00 </td></tr><tr><td> שאכר / חממה </td><td> ניו ברונסוויק מדעי </td><td> הסדרה הקלאסית C24 </td></tr><tr><td> טייפון סורק </td><td> GE Healthcare </td><td> 9200 משתנה מצב </td></tr><tr><td> סרכזת </td><td> Beckman Coulter </td><td> אלגרה X22-R </td></tr><tr><td> ספקטרופוטומטר UV </td><td> Thermo Scientific </td><td> GenesysUV 10 </td></tr><tr><td> הקרינה ספקטרופוטומטר </td><td> וריאן Inc </td><td> קרי ליקוי חמה </td></tr></tbody></table>

detection of bacteria using fluorogenic dnazymes

Outbreaks linked to food-borne and hospital-acquired pathogens account for millions of deaths and hospitalizations as well as colossal economic losses each and every year. Prevention of such outbreaks and minimization of the impact of an ongoing epidemic place an ever-increasing demand for analytical methods that can accurately identify culprit pathogens at the earliest stage. Although there is a large array of effective methods for pathogen detection, none of them can satisfy all the following five premier requirements embodied for an ideal detection method: high specificity (detecting only the bacterium of interest), high sensitivity (capable of detecting as low as a single live bacterial cell), short time-to-results (minutes to hours), great operational simplicity (no need for lengthy sampling procedures and the use of specialized equipment), and cost effectiveness. For example, classical microbiological methods are highly specific but require a long time (days to weeks) to acquire a definitive result.1 PCR- and antibody-based techniques offer shorter waiting times (hours to days), but they require the use of expensive reagents and/or sophisticated equipment.2-4 Consequently, there is still a great demand for scientific research towards developing innovative bacterial detection methods that offer improved characteristics in one or more of the aforementioned requirements. Our laboratory is interested in examining the potential of DNAzymes as a novel class of molecular probes for biosensing applications including bacterial detection.5
DNAzymes (also known as deoxyribozymes or DNA enzymes) are man-made single-stranded DNA molecules with the capability of catalyzing chemical reactions.6-8 These molecules can be isolated from a vast random-sequence DNA pool (which contains as many as 1016 individual sequences) by a process known as "in vitro selection" or "SELEX" (systematic evolution of ligands by exponential enrichment).9-16 These special DNA molecules have been widely examined in recent years as molecular tools for biosensing applications.6-8
Our laboratory has established in vitro selection procedures for isolating RNA-cleaving fluorescent DNAzymes (RFDs; Fig. 1) and investigated the use of RFDs as analytical tools.17-29 RFDs catalyze the cleavage of a DNA-RNA chimeric substrate at a single ribonucleotide junction (R) that is flanked by a fluorophore (F) and a quencher (Q). The close proximity of F and Q renders the uncleaved substrate minimal fluorescence. However, the cleavage event leads to the separation of F and Q, which is accompanied by significant increase of fluorescence intensity.
More recently, we developed a method of isolating RFDs for bacterial detection.5 These special RFDs were isolated to "light up" in the presence of the crude extracellular mixture (CEM) left behind by a specific type of bacteria in their environment or in the media they are cultured (Fig. 1). The use of crude mixture circumvents the tedious process of purifying and identifying a suitable target from the microbe of interest for biosensor development (which could take months or years to complete). The use of extracellular targets means the assaying procedure is simple because there is no need for steps to obtain intracellular targets.
Using the above approach, we derived an RFD that cleaves its substrate (FS1; Fig. 2A) only in the presence of the CEM produced by E. coli (CEM-EC).5 This E. coli-sensing RFD, named RFD-EC1 (Fig. 2A), was found to be strictly responsive to CEM-EC but nonresponsive to CEMs from a host of other bacteria (Fig. 3).
Here we present the key experimental procedures for setting up E. coli detection assays using RFD-EC1 and representative results.

Watch this Scientific Journal Video about Detection of Bacteria Using Fluorogenic DNAzymes at JoVE.com

Detection of Bacteria Using Fluorogenic DNAzymes

דיווחנו לאחרונה גישה חדשנית להפקת בדיקות DNAzyme fluorogenic כי ניתן להחיל על הקמת פשוט, &quot;תמהיל-and-לקרוא&quot; assay ניאון לגילוי חיידקי. אלו בדיקות דנ&quot;א מיוחד לזרז את המחשוף של מצע ה-DNA-RNA-chromophore שונה chimeric בנוכחות תערובת תאי גולמי (CEM) המיוצר על ידי חיידק מסוים, ובכך לתרגם את גילוי החיידק לדור אותות הקרינה. בדוח זה נתאר את הליכי הניסוי העיקריים שבהם בדיקה DNAzyme מסוים כונה &quot;RFD-EC1&quot; מועסק לצורך זיהוי של החיידק המודל,<emEscherichia coli&gt; (E. coli)</em&gt;.

זיהוי של חיידקים באמצעות DNAzymes Fluorogenic

Outbreaks linked to food-borne and hospital-acquired pathogens account for millions of deaths and hospitalizations as well as colossal economic losses ...

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Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce

שילוב של הדגימה דבק קלטת מבוססי פלואורסצנטי באתרו הכלאה עבור איתור מהיר של סלמונלה על תוצרת טרייה

This protocol describes a simple approach for adhesive-tape-based sampling of tomato and other fresh produce surfaces, followed by on-tape fluorescence in ...

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex זיהוי של חיידקים דוגמאות מורכבות קלינית הסביבה באמצעות oligonucleotide מצמידים פלורסנט microspheres

Bacterial vaginosis (BV) is a recurring polymicrobial syndrome that is characterized by a change in the "normal" microbiota from Lactobacillus-dominated ...

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

בעקבות בזמן אמת השפעת גורמי דלקת ריאות ארסיים בעכבר דלקת ריאות דגם אקוטי באמצעות חיידקי Bioluminescent

Pneumonia is one of the major health care problems in developing and industrialized countries and is associated with considerable morbidity and mortality. ...

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

זיהוי מהיר של חיידקים גראם שליליים מהדם תרבות מרק שימוש MALDI-TOF המוני ספקטרומטריית

An important role of the clinical microbiology laboratory is to provide rapid identification of bacteria causing bloodstream infection. Traditional ...

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

באתרו איתור של חיידקים בתוך רקמות מוטבעות-פרפין באמצעות DNA בדיקה שכותרת Digoxin מיקוד 16S rRNA

The presence of bacteria within the pocket epithelium and underlying connective tissue in gingival biopsies from patients with periodontitis has been ...

Biomimetic Materials to Characterize Bacteria-host Interactions

חומרי Biomimetic לאפיין אינטראקציות חיידקים-מארח

Bacterial attachment to host cells is one of the earliest events during bacterial colonization of host tissues and thus a key step during infection. The ...

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7

והקאה הפתוגן הקרנה באמצעות מגנטו-פלורסנט ננו-חיישן: איתור מהיר של e. Coli O157:H7

Enterohemorrhagic Escherichia coli O157:H7 has been linked to both waterborne and foodborne illnesses, and remains a threat despite the food- and ...

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

זיהוי העדינה של סמנים ביולוגיים באמצעות הטבעה מולקולרית מבוסס ביוסנסור קיבולי

The ability to detect and quantitate biomolecules in complex solutions has always been highly sought-after within natural science; being used for the ...

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

פלטפורמה תפוקה גבוהה להקרנה של סלמונלה spp /שיגלה spp.

Fecal-oral transmission of acute gastroenteritis occurs from time to time, especially when people who handled food and water are infected by Salmonella ...

Assaying for Inorganic Polyphosphate in Bacteria

Assaying עבור הפוליפוספאט אורגניים של חיידקים

Inorganic polyphosphate (polyP) is a biological polymer found in cells from all domains of life, and is required for virulence and stress response in many ...