Name: determining soil-transmitted helminth infection status and physical fitness of school-aged children
Uploaded: 2012-08-22T12:00:00
Description: Watch this Scientific Journal Video about Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children at JoVE.com

Forfatterne er taknemmelige for de forskellige befolkningsgrupper i Yunnan provinsen, Folkerepublikken Kina og Agboville, Côte d&#39;Ivoire, for deres deltagelse i de ovenfor beskrevne undersøgelser. Vi står i gæld til det lokale felt teams, der har bistået i afprøvningen og gennemførelsen af ​​protokollerne. Vi er især taknemmelige til følgende kolleger fra det schweiziske Tropiske og Public Health Institute: Yvette Endriss for at tilvejebringe de mikroskopiske billeder, Hanspeter Marti og hans team til laboratorie support og Stefanie Krauth for hendes hjælp under video optagelserne. Endelig takker vi de to anonyme dommere til en række nyttige kommentarer.

1. Kato-Katz Teknik <ol><li> Anbring en standard Kato-Katz skabelon med et hul til fastholdelse 41,7 milligram (mg) af fæces på et mikroskopobjektglas. </li><li> Scoop 2-3 gram (g) af en frisk fækal prøve på et stykke avis eller aluminiumfolie og trykke et stykke ståltråd eller plastnet oven at sigte det. </li><li> Anvendelse af en lille spatel, skrabe den sigtede materiale fra masken og helt udfylde hullet i Kato-Katz skabelon med det. For at fjerne overskydende fækalt materiale, nivellere indholdet af hullet...

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De tre protokoller er beskrevet i dette dokument er blevet testet og udført i den syd-vestlige del af Yunnan-provinsen, Folkerepublikken Kina blandt medlemmer af Bulang etniske mindretal 21 og i forskellige dele af Afrika 6,13,14. Der er flere skabeloner til rådighed for Kato-Katz teknik. Hvert enkelt hul-størrelse vil levere en særskilt mængde af fækalt materiale. Til beregning af EPG&#39;er er det derfor nødvendigt at kende enhed af skabelonen, således at mult...

Bemærk: Materialer på denne liste er generiske og kan fås fra forskellige kilder. <table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer </td></tr><tr><td> Kato-Katz kit [400 plast skabeloner med et hul på 6 millimeter (mm) (diameter) i en 1,5 mm tyk skabelon, 400 plastic spatel, en 20 m rolle nylonnet på 80 maskestørrelse, en 20 m rolle hydrofil cellofan, 34 um tykke.] </td><td> Vestergaard Frandsen </td><td></td><td> Bemærk venligst, at glycerin-malakitgrønt løsning ikke er inkluderet i dette kit, men kan købes fra nogen kemisk leverandør. </td></tr><tr><td> Glastragt [omkring 8 centimeter (cm) bred og 6 cm dyb] </td><td></td><td></td><td> The glastragt for Baermann teknikken skal kunne rumme omkring 60 ml vand. The størrelse gummislange og gaze afhænger af den anvendte type glastragt. </td></tr><tr><td> Biologisk mikroskop </td><td> Olympus </td><td> CX21LED/CX21 </td><td></td></tr><tr><td> Centrifuger </td><td> Shanghai Medical Instruments Group </td><td> 80 - 2T </td><td></td></tr><tr><td> Indspillede signaler </td><td> Bitworks Design </td><td> Team Bleep Test Version 1.3.1 </td><td> iPhone-applikation </td></tr><tr><td> Pulsur </td><td> POLAR </td><td> FT1 </td><td> Watch og transmitter </td></tr></tbody></table>

determining soil-transmitted helminth infection status and physical fitness of school-aged children

Jord-transmitterede helminther (STH) infektioner er almindelige. Faktisk er mere end 1 milliard mennesker ramt, især i udviklingslandene, hvor fattigdommen hersker og hygiejne adfærd, vandforsyning og sanitet er ofte mangelfuld 1,2. Ascaris lumbricoides, Trichuris trichiura, og de ​​to hageorm arter, Ancylostoma duodenale og Necator americanus, er den mest udbredte STHs 3. Den estimerede globale byrde på grund af hageorm sygdom, ascariasis, og trichuriasis er 22,1, 10,5, og 6,4 mio disability-adjusted life years (DALY), henholdsvis 4. Desuden er en anslået 30 til 100.000.000 mennesker smittet med Strongyloides stercoralis, de mest forsømte STH arter af global betydning, som velsagtens også forårsager en betydelig indvirkning på folkesundheden 5,6. Multiple-arter infektioner (dvs. forskellige STHs nærede i et enkelt individ) er fælles, og infektioner har været lin<html> KED til sænket produktivitet og dermed økonomiske udsigter i udviklingslandene 1,3. Til diagnosticering af fælles STHs, anbefaler World Health Organization (WHO) den Kato-Katz teknik 7,8, hvilket er en forholdsvis enkel metode til bestemmelse af forekomsten og intensiteten af sådanne infektioner. Det letter påvisning af parasitæg at smittede forsøgspersoner passerer i deres afføring. Med hensyn til diagnosticering af S.stercoralis, er der i øjeblikket ingen enkel og præcist værktøj til rådighed. The Baermann teknik er den mest anvendte fremgangsmåde til diagnose. Princippet bag Baermann teknik er, at aktive S.stercoralis larver migrere ud af en belyst frisk fækal prøve som larverne er phototactic 9. Det kræver mindre avancerede laboratorie materialer og er mindre tidskrævende end kultur og immunologiske metoder 5. t &quot;&gt; morbiditeter associeret med STH infektioner spænder fra akutte, men fælles symptomer, såsom mavesmerter, diarré og kløe, til kroniske symptomer, såsom anæmi, under-og fejlernæring, og kognitiv svækkelse 10. Da symptomerne er generelt uspecifikke og subtile, de ofte ubemærket, betragtes som en normal tilstand med ramte individer eller behandles som symptomer på andre sygdomme, der kan være mere almindelige i en given indstilling. Det er således tænkeligt, at den sande byrde STH infektioner er undervurderet med vurdering værktøjer bygger på selv-deklarerede tegn og symptomer, som normalt er tilfældet i befolkningsbaserede undersøgelser. I slutningen af 1980&#39;erne og begyndelsen af 1990&#39;erne, fremhævede Stephenson og kolleger mulighed for STH infektioner sænker fysiske egnethed drenge i alderen 6-12 år 11,12. Denne linje af videnskabelig undersøgelse fået ny fremdrift for nylig 13,14,15. Den 20-meter (m) shuttle run test blev udviklet end valideret af Léger et al. 16 og anvendes i hele verden til at måle aerob kondition af børn 17. Testen er nem at standardisere og kan udføres hvor en 20-meter lang og flad kører kursus og en lydkilde er til rådighed, der gør brugen attraktiv i ressourcerige begrænsede indstillinger 13. For at lette og standardisere forsøg på at vurdere, om STH infektioner have en effekt på den fysiske egnethed skolebørn, præsenterer vi metoder til at diagnosticere STH infektioner eller foranstaltning fysisk fitness, der er nemme at udføre og alligevel giver nøjagtige og reproducerbare resultater. Dette vil bidrage til at skabe nye beviser vedrørende de sundhedsmæssige konsekvenser af STH infektioner.

Watch this Scientific Journal Video about Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children at JoVE.com

Determining Soil-transmitted Helminth Infection Status and Physical Fitness of School-aged Children

Kronisk infektion med jord-transmitteres helminter (STHs) forårsager malabsorption, forkrøbling, og spilder på det voksende barn. Derfor er det sandsynligt, at disse infektioner også reducere fysiske egnethed for børn. Her vil vi visualisere to teknikker til diagnosticering af STHs og den 20-meter shuttle run test for at vurdere børns fysiske kondition.

Bestemmelse Jord-transmitteret Helminth Infektion status og fysiske egnethed skolebørn

Soil-transmitted helminth (STH) infections are common. Indeed, more than 1 billion people are affected, mainly in the developing world where poverty ...

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