Name: high-throughput synthesis of carbohydrates and functionalization of polyanhydride nanoparticles
Uploaded: 2012-07-06T12:00:00
Description: Watch this Scientific Journal Video about High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles Video at JoVE.com

Forfatterne ønsker å takke den amerikanske Army Medical Research og forsyningskommando Command (Grant # W81XWH-10-1-0806) og National Institutes of Health (Grant # U19 AI091031-01 og Grant # 1R01GM090280) for økonomisk støtte. BN erkjenner Balloun professoratet i kjemisk og biologisk Engineering og NLBP erkjenner Wilkinson professoratet i Tverrfaglig Engineering. Vi takker Julia Vela for bistand henne i å utføre nanopartikkel funksjonalisering eksperimenter.

1. High-throughput Karbohydrat Synthesis <ol><li> Før den automatiserte syntese av dimannoside, et passende beskyttet sukker donor, typisk trichloroacetimidate, og akseptor, hovedsakelig en alkenyl fluorous alkohol, er syntetisert på benken-top. </li><li> Et program er skrevet for automatisert syntese av dimannoside. En skjematisk fremstilling av den grunnleggende automatisert prosedyren er presentert i figur 2. I programmet er det sikret at før tillegg av arrangøren, er blandingen av donor og aks...

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Effekten av karbohydrater som målretting agenter til direkte nanopartikkel interaksjoner til immunceller tidligere har blitt vist 10, 11. Tidligere forskning i våre laboratorier har vist at spesifikke sukker knyttet til polyanhydride nanopartikler er i stand til å målrette ulike CLRs på antigenpresenterende celler (APC), og dermed styrke aktivering av immunceller som kan være viktig for videre T-celleaktivering 10, 11. Men for å oppnå optimal målretting flere parametre, for eksempel den po...

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high-throughput synthesis of carbohydrates and functionalization of polyanhydride nanoparticles

Transdisiplinær tilnærminger som involverer områder som materiale design, nanoteknologi, kjemi og immunologi må utnyttes til rasjonelt utforme effektive vaksiner bærere. Nanopartikkel-baserte plattformer kan forlenge varigheten av vaksinen antigener, noe som kan forbedre vaksinen immunogenisitet en. Flere biologisk nedbrytbare polymerer har vært studert som vaksine levering biler 1; spesielt, har polyanhydride partikler vist evne til å gi vedvarende utgivelsen av stabile protein antigener og for å aktivere antigenpresenterende celler og modulere immunresponsen 2-12. Den molekylære utformingen av disse vaksine bærere må integrere rasjonelle valg av polymer egenskaper samt inkorporering av hensiktsmessige målretting agenter. Høy gjennomstrømning automatisert fabrikasjon av målretting ligander og functionalized partikler er et kraftig verktøy som vil forbedre evnen til å studere et bredt range av eiendommer og vil føre til utformingen av reproduserbare vaksine levering enheter. Tilsetting av målretting ligander som kan bli gjenkjent av spesifikke reseptorer på immunceller har vist seg å modulere og skreddersy immunresponser 10,11,13 C-type lektin reseptorer (CLRs) er mønstergjenkjenning reseptorer (PRRs) som anerkjenner karbohydrater tilstede på overflaten av patogener. Stimulering av immunceller via CLRs gir forbedret internalisering av antigen og påfølgende presentasjon for ytterligere T-celleaktivering 14,15. Derfor karbohydrat molekylene spiller en viktig rolle i studiet av immunreaksjoner, men bruken av disse biomolekyler ofte lider av mangel på tilgjengeligheten av strukturelt veldefinerte og rene karbohydrater. En automatisering plattform basert på iterativ løsning-fase reaksjoner kan muliggjøre rask og kontrollert syntese av disse syntetisk utfordrende molekylene bruker vesentlig lavere building blokk mengder enn tradisjonelle solid fase metoder 16,17. Heri vi rapporterer en protokoll for automatisert løsning-fase syntese av oligosakkarider som mannose-baserte rettet ligander med fluorous fast-fase ekstraksjon for mellomlagring rensing. Etter utvikling av automatiserte metoder for å gjøre karbohydrat-baserte målretting agent, beskriver vi metoder for feste sin på overflaten av polyanhydride nanopartikler ansette en automatisert robot satt opp betjenes av LabVIEW som tidligere beskrevet 10. Overflate funksjonalisering med karbohydrater har vist effekt i målretting CLRs 10,11 og øke gjennomstrømningen av fabrikasjon metode for å avdekke kompleksiteten forbundet med en multi-parametrisk system vil være av stor verdi (Figur 1a).

Watch this Scientific Journal Video about High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles Video at JoVE.com

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles Video (Video) | JoVE

I denne artikkelen, er en høy gjennomstrømning metode presenteres for syntese av oligosakkarider og deres tilknytning til overflaten av polyanhydride nanopartikler for videre bruk i målretting spesifikke reseptorer på antigenpresenterende celler.

High-throughput syntese av karbohydrater og funksjonalisering av Polyanhydride Nanopartikler

Transdisciplinary approaches involving areas such as material design, nanotechnology, chemistry, and immunology have to be utilized to rationally design ...

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High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

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