Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

授予的“德意志研究联合会”（EI 866/1-1）苏艾森哈特支持这项工作。

1。麻醉和监测<ol><li>适当的国家和机构伦理应该在之前进行动物实验。继批准从伦理委员会麻醉男性只SD大鼠的体重从120 - 180克。提供2 - 3％异氟醚，异氟醚蒸发器和一个有机玻璃箱，通过放置在大鼠内。 </li><li>尽快达到适当的麻醉水平（缺乏反应脚趾或尾巴捏）大鼠加权和腹侧颈区剃光。 </li><li>放在一个加热垫在背卧鼠体温保持在37°C和异氟醚适用于2卷用硅胶面具％。 </li></ol><p class="jove_co...

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	<li>Cetin, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+fucoidin+(a+neutrophil+rolling+inhibitor)+on+ischemia+reperfusion+injury:+experimental+study+in+rat+epigastric+island+flaps.">Protective effect of fucoidin (a neutrophil rolling inhibitor) on ischemia reperfusion injury: experimental study in rat epigastric island flaps.</a> Ann. Plast. Surg. 47, 540-546 (2001).</li><li>Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+xanthine+oxidase+and+granulocytes+in+ischemia-reperfusion+injury.">Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury.</a> Am. J. Physiol. 255, H1269-H1275 (1988).</li><li>Lazarus, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+mast+cells+in+ischaemia-reperfusion+injury+in+murine+skeletal+muscle.">The role of mast cells in ischaemia-reperfusion injury in murine skeletal muscle.</a> J Pathol. 191, 443-448 (2000).</li><li>van den Heuvel, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Review:+Ischaemia-reperfusion+injury+in+flap+surgery.">Review: Ischaemia-reperfusion injury in flap surgery.</a> J. Plast. Reconstr. Aesthet. Surg. 62, 721-726 (2009).</li><li>Rosen, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+surface+lectins+in+the+immune+system.">Cell surface lectins in the immune system.</a> Semin. Immunol. 5, 237-247 (1993).</li><li>van der Flier, A., Sonnenberg, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Function+and+interactions+of+integrins.">Function and interactions of integrins.</a> Cell Tissue Res. 305, 285-298 (2001).</li><li>Panes, J., Perry, M., Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Leukocyte-endothelial+cell+adhesion:+avenues+for+therapeutic+intervention.">Leukocyte-endothelial cell adhesion: avenues for therapeutic intervention.</a> Br. J. Pharmacol. 126, 537-550 (1999).</li><li>Gavins, F. N., Chatterjee, B. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravital+microscopy+for+the+study+of+mouse+microcirculation+in+anti-inflammatory+drug+research:+focus+on+the+mesentery+and+cremaster+preparations.">Intravital microscopy for the study of mouse microcirculation in anti-inflammatory drug research: focus on the mesentery and cremaster preparations.</a> J. Pharmacol. Toxicol. Methods. 49, 1-14 (2004).</li><li>Sutton, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injury+of+the+renal+microvascular+endothelium+alters+barrier+function+after+ischemia.">Injury of the renal microvascular endothelium alters barrier function after ischemia.</a> Am. J. Physiol. Renal. Physiol. 285, 191-198 (2003).</li><li>Serracino-Inglott, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+nitric+oxide+synthase+expression+during+hepatic+ischemia-reperfusion.">Differential nitric oxide synthase expression during hepatic ischemia-reperfusion.</a> Am. J. Surg. 185, 589-595 (2003).</li><li>Eppinger, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mediators+of+ischemia-reperfusion+injury+of+rat+lung.">Mediators of ischemia-reperfusion injury of rat lung.</a> Am J Pathol. 150, 1773-1784 (1997).</li><li>Dumont, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+imaging+of+apoptotic+cell-membrane+changes+at+the+single-cell+level+in+the+beating+murine+heart.">Real-time imaging of apoptotic cell-membrane changes at the single-cell level in the beating murine heart.</a> Nat Med. 7, 1352-1355 (2001).</li><li>Baez, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+open+cremaster+muscle+preparation+for+the+study+of+blood+vessels+by+in+vivo+microscopy.">An open cremaster muscle preparation for the study of blood vessels by in vivo microscopy.</a> Microvasc Res. 5, 384-394 (1973).</li><li>Woeste, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Octreotide+attenuates+impaired+microcirculation+in+postischemic+pancreatitis+when+administered+before+induction+of+ischemia.">Octreotide attenuates impaired microcirculation in postischemic pancreatitis when administered before induction of ischemia.</a> Transplantation. 86, 961-967 (2008).</li><li>Schultz, J. E., Hsu, A. K., Gross, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphine+mimics+the+cardioprotective+effect+of+ischemic+preconditioning+via+a+glibenclamide-sensitive+mechanism+in+the+rat+heart.">Morphine mimics the cardioprotective effect of ischemic preconditioning via a glibenclamide-sensitive mechanism in the rat heart.</a> Circ. Res. 78, 1100-1104 (1996).</li><li>Dobschuetz, E. v. o. n. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+intravital+fluorescence+microscopy--a+novel+method+for+the+assessment+of+microvascular+permeability+in+acute+pancreatitis.">Dynamic intravital fluorescence microscopy--a novel method for the assessment of microvascular permeability in acute pancreatitis.</a> Microvasc Res. 67, 55-63 (2004).</li><li>Vutskits, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adverse+effects+of+methylene+blue+on+the+central+nervous+system.">Adverse effects of methylene blue on the central nervous system.</a> Anesthesiology. 108, 684-692 (2008).</li><li>Takasu, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+survival+time+with+combined+early+blood+transfusion+and+fluid+administration+in+uncontrolled+hemorrhagic+shock+in+rats.">Improved survival time with combined early blood transfusion and fluid administration in uncontrolled hemorrhagic shock in rats.</a> J. Trauma. 8, 312-316 (2010).</li><li>Proctor, K. G., Busija, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationships+among+arteriolar,+regional,+and+whole+organ+blood+flow+in+cremaster+muscle.">Relationships among arteriolar, regional, and whole organ blood flow in cremaster muscle.</a> Am. J. Physiol. 249, 34-41 (1985).</li><li>Bagher, P., Segal, S. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Mouse+Cremaster+Muscle+Preparation+for+Intravital+Imaging+of+the+Microcirculation.">The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation.</a> J. Vis. Exp. (52), e2874 (2011).</li><li>Kanwar, S., Hickey, M. J., Kubes, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postischemic+inflammation:+a+role+for+mast+cells+in+intestine+but+not+in+skeletal+muscle.">Postischemic inflammation: a role for mast cells in intestine but not in skeletal muscle.</a> Am. J. Physiol. 275, 212-218 (1998).</li><li>Leoni, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflamed+phenotype+of+the+mesenteric+microcirculation+of+melanocortin+type+3+receptor-null+mice+after+ischemia-reperfusion.">Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion.</a> FASEB J. 22, 4228-4238 (2008).</li><li>Simoncini, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interaction+of+oestrogen+receptor+with+the+regulatory+subunit+of+phosphatidylinositol-3-OH+kinase.">Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase.</a> Nature. 407, 538-541 (2000).</li><li>Woollard, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathophysiological+levels+of+soluble+P-selectin+mediate+adhesion+of+leukocytes+to+the+endothelium+through+Mac-1+activation.">Pathophysiological levels of soluble P-selectin mediate adhesion of leukocytes to the endothelium through Mac-1 activation.</a> Circ. Res. 103, 1128-1138 (2008).</li><li>Mori, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ischemia-reperfusion+induced+microvascular+responses+in+LDL-receptor+-/-+mice.">Ischemia-reperfusion induced microvascular responses in LDL-receptor -/- mice.</a> Am. J. Physiol. 276, H1647-H1654 (1999).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+Molecular+Changes+Induced+by+Ischemia/Reperfusion+in+Human+Free+Muscle+Flap+Tissue+Samples.">Monitoring Molecular Changes Induced by Ischemia/Reperfusion in Human Free Muscle Flap Tissue Samples.</a> Ann. Plast. Surg. , (2011).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+activation-specific+human+anti-{alpha}M{beta}2+single-chain+antibodies+as+potential+diagnostic+tools+and+therapeutic+agents.">Generation of activation-specific human anti-{alpha}M{beta}2 single-chain antibodies as potential diagnostic tools and therapeutic agents.</a> Blood. 109, 3521-3528 (2007).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissociation+of+pentameric+to+monomeric+C-reactive+protein+on+activated+platelets+localizes+inflammation+to+atherosclerotic+plaques.">Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.</a> Circ Res. 105, 128-137 (2009).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C-reactive+protein:+how+conformational+changes+influence+inflammatory+properties.">C-reactive protein: how conformational changes influence inflammatory properties.</a> Cell Cycle. 8, 3885-3892 (2009).</li><li>Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Physiology and pathophysiology of leukocyte adhesion. , 520 (1995).</li><li>Baatz, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kinetics+of+white+blood+cell+staining+by+intravascular+administration+of+rhodamine+6G.">Kinetics of white blood cell staining by intravascular administration of rhodamine 6G.</a> Int. J. Microcirc. Clin. Exp. 15, 85-91 (1995).</li><li>Mempel, T. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+imaging+of+leukocyte+trafficking+in+blood+vessels+and+tissues.">In vivo imaging of leukocyte trafficking in blood vessels and tissues.</a> Curr. Opin. Immunol. 16, 406-417 (2004).</li><li>Abbitt, K. B., Rainger, G. E., Nash, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+fluorescent+dyes+on+selectin+and+integrin-mediated+stages+of+adhesion+and+migration+of+flowing+leukocytes.">Effects of fluorescent dyes on selectin and integrin-mediated stages of adhesion and migration of flowing leukocytes.</a> J. Immunol. Methods. 239, 109-119 (2000).</li></ol>

白细胞-内皮细胞相互作用，生产活性氧和激活补体系统是IRI的诱导组织功能障碍的主要特点。26受影响组织的微循环被视为不可分割的炎症发病网站。除了 ​​如流室检测27，28的 体外实验，它是强制性的，以提供行之有效的活体成像模型，以进一步评估在体内相关。虽然已在不同的器官系统有牵连IRI的，我们在这里描述的方法系统地研究白细胞与内皮细胞相互作用...

<table border="1"><tbody><tr><td> 设备名称： </td><td> 公司名称： </td><td> 目录号： </td><td> 评论： </td></tr><tr><td> forene 100％（V / V） </td><td>住持</td><td> B506 </td><td> API的异氟醚</td></tr><tr><td>涤纶缝合</td><td> serag Weissner </td><td> OC108000 </td><td></td></tr><tr><td> portex精细孔聚乙烯管材</td><td>史密斯医疗</td><td> 800/100/100 </td><td> 0.28毫米内径</td></tr><tr><td> 0.9％的生理盐水溶液</td><td> fresinus卡比</td><td> 808771 </td><td></td></tr><tr><td>变化的一个尖端豪华烧灼套件的</td><td> bovie医学</td><td>警报1动作延迟时间</td><td></td></tr><tr><td> abbocath-14Gţ </td><td> venisystems </td><td> G713- A01 </td><td>作为镜头筒</td></tr><tr><td>伺服呼吸机900C </td><td> MAQUET </td><td></td><td>用作动物ventialtor的</td></tr><tr><td>逻辑压力传感器</td><td>史密斯医疗</td><td> MX1960 </td><td></td></tr><tr><td> sirecust 404显示器</td><td>西门子</td><td></td><td></td></tr><tr><td> ABL的700台式分析仪</td><td>辐射计</td><td></td><td>血液气体测量</td></tr><tr><td>加热垫</td><td> effenberger </td><td> 8319 </td><td></td></tr><tr><td>铝合金舞台</td><td> alfun </td><td> AW7022 </td><td></td></tr><tr><td>手术显微镜OPMI 6 SDFC </td><td>卡尔·蔡司</td><td></td><td></td></tr><tr><td>显微仪器实验室小号等</td><td>科技</td><td> 767 </td><td></td></tr><tr><td> biemer船只夹</td><td>迪纳</td><td> 64.562 </td><td></td></tr><tr><td>应用镊子</td><td>迪纳</td><td> 64.568 </td><td>为Biemer血管夹</td></tr><tr><td>罗丹明6G </td><td> Sigma-Aldrich公司</td><td> R4127 </td><td></td></tr><tr><td>凡士林白民建联</td><td>温斯洛普</td><td> 2726853 </td><td></td></tr><tr><td>盖玻片</td><td></td><td></td><td> 32×32毫米</td></tr><tr><td></td><td></td><td></td><td> 活体设置 </td></tr><tr><td> zeis Axio上范围A-1中太</td><td>卡尔Zeis </td><td> 490036 </td><td>萤光显微镜</td></tr><tr><td> 470 nm的LED </td><td>卡尔Zeis </td><td> 423052 </td><td>荧光光源</td></tr><tr><td>科利柏2系统</td><td>卡尔Zeis </td><td> 423052 </td><td></td></tr><tr><td> W计划的复消色差透镜20X / 1,0迪爱生</td><td>卡尔Zeis </td><td> 421452 </td><td>浸水目标</td></tr><tr><td> AxioCam MRM牧师3火线</td><td>卡尔Zeis </td><td> 426509 </td><td>高清晰度的数码相机</td></tr><tr><td> AXIO视力LE软件</td><td>卡尔Zeis </td><td> 410130 </td><td>用于离线分析</td></tr></tbody></table>

real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

在病理条件下，如脑卒中，心肌梗死，肠缺血，以及移植和心血管手术。1以前缺血组织的再灌注后的大阵，而必要的预防不可逆转的缺血再灌注损伤（IRI）有牵连组织损伤，诱发过度炎症反应受影响的组织。毗邻的活性氧，活化补体系统的生产和增加血管通透性，激活白细胞再灌注过程中的病理级联在炎症组织损伤的原则演员之一。2，3白细胞激活是一个多步过程组成轧制，坚定的粘附和轮回之间复杂的相互作用的粘附分子介导的反应，如补体因子，趋化因子，血小板活化因子的趋化因子。 &lt;虽然在毛细血管静脉的白细胞滚动主要选择素5的相互作用介导，与他们的柜台配体，坚定白细胞粘附血管内皮细胞是通过粘附分子（ICAM）与血管细胞结合的选择控制类p =“的jove_content”&gt;粘附分子（VCAM），6，7 在体内的白细胞-内皮细胞相互作用观察的金标准是活体显微镜技术，首次在1968年8。 虽然各个器官缺血再灌注损伤（缺血再灌注损伤）的各种型号，9-12只有少数是适合微血管床的白细胞招聘的直接可视化的图像质量高的水平上。 在这里，我们促进提睾微循环的毛细血管后微静脉的活体荧光显微镜的数字作为一种方便的方法大鼠IRI的研究，定性和定量分析白细胞招聘横纹肌组织，并为实现该技术提供了一个详细的手册13。我们进一步说明常见的陷阱，应该使读者能够真正体会，安全地执行方法，并提供有用的提示。 在由步步协议，我们描述如何获得足够的监测，保持时间较长的动物坚决麻醉呼吸控制麻醉下开始。然后，我们描述优秀的光学分辨率为薄平板提睾准备和提供已在我们的实验室建立在IRI白细胞成像的协议。

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

数字提睾微循环毛细血管静脉活体荧光显微镜是一种方便的方法获得白细胞 - 内皮细胞相互作用的见解<em&gt;在体内</em在缺血再灌注损伤（IRI）的横纹肌组织。我们在这里提供一份详细的协议，安全地执行技术，并讨论了它的应用和限制。

实时数字成像大鼠提睾肌缺血再灌注损伤（IRI）的白细胞 - 内皮细胞相互作用

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

Research

JoVE Journal

Medicine

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

在原位横向腹直肌肌皮瓣：肌缺血再灌注损伤模型大鼠

Free tissue transfer is the gold standard of reconstructive surgery to repair complex defects not amenable to local options or those requiring composite ...

科研

医学

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

急性肾损伤和伤后纤维化小鼠缺血再灌注模型

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

心肌缺血再灌注损伤的通过左前降支结扎小鼠模型

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

诱导和缺血再灌注损伤评估中的Langendorff灌注鼠心

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

肠缺血再灌注损伤的小鼠模型

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

在鼠耳皮肤的免疫反应的活体多光子成像诱导缺血再灌注损伤

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

大鼠心肌缺血再灌注后冠状动脉流动储备评价

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

重建显微外科缺血再灌注损伤研究的临床前大鼠模型

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

在穆林模型中缺血再灌注损伤后干细胞的心肌内传递延迟

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

器官缺血-输液损伤模拟大鼠肝脏移植模型的血动力学变化

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...