Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Questo lavoro è stato sostenuto da una concessione del &quot;Deutsche Forschungsgemeinschaft&quot; a SU Eisenhardt (EI 866/1-1).

1. Anestesia e monitoraggio <ol><li> Etico competente nazionale e istituzionale dovrebbe essere a posto prima di effettuare esperimenti su animali. Dopo l&#39;approvazione al comitato etico anestetizzare ratti Sprague Dawley maschi con un peso corporeo 120-180 g. Consegna 2 - 3% vol isoflurano a una finestra di plexiglas con vaporizzatore isoflurano e posizionare all&#39;interno ratto. </li><li> Non appena il livello adeguato di anestesia si ottiene (mancanza di reazione a punta o la coda pizzico), il ratto è pondera...

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Leucociti-endotelio, la produzione di specie reattive dell&#39;ossigeno e l&#39;attivazione del sistema del complemento sono le caratteristiche principali di IRI indotta disfunzione tessuto. 26 La microcircolazione del tessuto colpito è considerato come il sito integrale per l&#39;insorgenza infiammatoria. A parte gli esperimenti ex vivo come test camera di flusso 27, 28, è obbligatorio fornire consolidati modelli di imaging intravitale di valutare ulteriormente la rilevanza in vivo....

<table border="1"><tbody><tr><td> Nome del prodotto: </td><td> Società: </td><td> N. di catalogo: </td><td> Commenti: </td></tr><tr><td> Forene 100% (V / V) </td><td> Abate </td><td> B506 </td><td> API isoflurane </td></tr><tr><td> Terylene sutura </td><td> Serag Weissner </td><td> OC108000 </td><td></td></tr><tr><td> Portex Belle Bore tubi in polietilene </td><td> Smiths Medical </td><td> 800/100/100 </td><td> 0,28 mm diametro interno </td></tr><tr><td> 0,9% soluzione salina </td><td> Fresinus Kabi </td><td> 808771 </td><td></td></tr><tr><td> Change-A-tip deluxe kit cautery </td><td> Bovie Medical </td><td> Del1 </td><td></td></tr><tr><td> Abbocath-T 14G </td><td> Venisystems </td><td> G713- A01 </td><td> utilizzato come tubo lente </td></tr><tr><td> Servo Ventilatore 900C </td><td> Maquet </td><td></td><td> utilizzati come ventialtor animale </td></tr><tr><td> Trasduttore di pressione Logical </td><td> Smiths Medical </td><td> MX1960 </td><td></td></tr><tr><td> Sirecust 404 Monitor </td><td> Siemens </td><td></td><td></td></tr><tr><td> ABL 700 da banco Analyzer </td><td> Radiometro </td><td></td><td> per la misurazione del gas sangue </td></tr><tr><td> Riscaldamento pad </td><td> Effenberger </td><td> 8319 </td><td></td></tr><tr><td> Alluminio stage </td><td> ALFUN </td><td> AW7022 </td><td></td></tr><tr><td> Chirurgica microscopio OPMI 6-SDFC </td><td> Carl Zeiss </td><td></td><td></td></tr><tr><td> Microchirurgica strumenti di laboratorio set </td><td> S &amp; T </td><td> 767 </td><td></td></tr><tr><td> Biemer nave Clip </td><td> Diener </td><td> 64,562 </td><td></td></tr><tr><td> L&#39;applicazione di forcipe </td><td> Diener </td><td> 64,568 </td><td> per clip nave Biemer </td></tr><tr><td> Rodamina 6G </td><td> Sigma-Aldrich </td><td> R4127 </td><td></td></tr><tr><td> Vaselina bianca DAB </td><td> Winthrop </td><td> 2726853 </td><td></td></tr><tr><td> Coprire gli occhiali </td><td></td><td></td><td> 32x32 millimetri </td></tr><tr><td></td><td></td><td></td><td> Installazione intravitale </td></tr><tr><td> Zeis Axio Scope A-1 MAT </td><td> Carl Zeis </td><td> 490036 </td><td> microscopio a epifluorescenza </td></tr><tr><td> 470 nm LED </td><td> Carl Zeis </td><td> 423052 </td><td> fluorescenza sorgente luminosa </td></tr><tr><td> Colibri 2 Sistema </td><td> Carl Zeis </td><td> 423052 </td><td></td></tr><tr><td> W Plan-Apochromat 20x / 1,0 DIC </td><td> Carl Zeis </td><td> 421452 </td><td> immersione in acqua obiettivo </td></tr><tr><td> AxioCam MRm Rev. 3 FireWire </td><td> Carl Zeis </td><td> 426509 </td><td> fotocamera digitale ad alta risoluzione </td></tr><tr><td> Axio LE vision software </td><td> Carl Zeis </td><td> 410130 </td><td> utilizzare per l&#39;analisi offline </td></tr></tbody></table>

real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

L&#39;ischemia-riperfusione (IRI) è stata implicata in una vasta gamma di condizioni patologiche come ictus cerebrale, infarto del miocardio, ischemia intestinale così come a seguito di trapianto e chirurgia cardiovascolare. 1 riperfusione di tessuto precedentemente ischemico, mentre essenziale per la prevenzione di irreversibile lesione dei tessuti, provoca infiammazione eccessiva del tessuto colpito. Adiacente alla produzione di specie reattive dell&#39;ossigeno, l&#39;attivazione del sistema del complemento e aumento della permeabilità microvascolare, l&#39;attivazione di leucociti è uno degli attori principali nella cascata patologica del danno tissutale infiammatorio durante la riperfusione. 2, 3 attivazione dei leucociti è un processo multifasico che consiste di laminazione, adesione e la ferma ed è mediata da una complessa interazione tra molecole di adesione in risposta a fattori chemiotattici quali fattori del complemento, chemochine, o fattore attivante le piastrine 4. &lt;p class = &quot;jove_content&quot;&gt; Mentre rolling leucociti in venule postcapillari è mediato principalmente dall&#39;interazione di selectine 5 con loro ligandi contatore, ferma adesione dei leucociti all&#39;endotelio selectina è controllato tramite legame a molecole di adesione intercellulare (ICAM) e vascolare cellulari molecole di adesione (VCAM) 6, 7. Gold standard per l&#39;osservazione in vivo di leucociti-endotelio è la tecnica della microscopia intravitale, descritta per la prima nel 1968 8. Benché vari modelli di IRI (ischemia-riperfusione) sono stati descritti per vari organi, 9-12 solo pochi sono adatti per la visualizzazione diretta del reclutamento di leucociti nel microcircolo a un alto livello di qualità dell&#39;immagine. 8 Abbiamo qui promuovere la microscopia digitale intravitale epifluorescenza del venule postcapillari del microcircolo cremastericodel ratto 13 come un metodo conveniente per analizzare qualitativamente e quantitativamente il reclutamento dei leucociti per la ricerca IRI-nel tessuto muscolare striato e fornire un manuale dettagliato per realizzare la tecnica. Abbiamo inoltre illustrare le insidie ​​più comuni e fornire consigli utili che dovrebbe consentire al lettore di apprezzare veramente, e sicuro di eseguire il metodo. In un passo dal protocollo passo che raffigurano come iniziare con l&#39;anestesia respirazione controllata sotto il controllo sufficiente a mantenere l&#39;animale saldamente anestetizzato per lunghi periodi di tempo. Abbiamo poi descrivere la preparazione cremasterico come un sottile foglio appartamento in eccellente risoluzione ottica e fornisce un protocollo per l&#39;imaging dei leucociti in IRI che è stato ben stabilito nei nostri laboratori.

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

Digital microscopia intravitale epifluorescenza delle venule postcapillari nella microcircolazione cremasterico è un metodo comodo per acquisire conoscenze in leucociti-endotelio<em&gt; In vivo</em&gt; In ischemia-riperfusione (IRI) del tessuto muscolare striato. Noi qui forniamo un protocollo dettagliato per eseguire in sicurezza la tecnica e discutere le sue applicazioni e limitazioni.

In tempo reale Imaging digitale di leucociti-endotelio in ischemia-riperfusione (IRI) del muscolo Cremaster Rat

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

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Medicine

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ trasversale retto addominale miocutaneo Flap: un modello di ratto di miocutaneo ischemia riperfusione

Free tissue transfer is the gold standard of reconstructive surgery to repair complex defects not amenable to local options or those requiring composite ...

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Medicina

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Ischemia-riperfusione Modello di danno renale acuta e post Infortunio fibrosi nei topi

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

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Un modello murino di ischemia miocardica e riperfusione lesioni da legatura della arteria discendente anteriore sinistra

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

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Induzione e valutazione di ischemia-riperfusione Injury in Langendorff-perfusi Hearts Rat

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

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Modello murino di ischemia-riperfusione intestinale Injury

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

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Inducendo ischemia-riperfusione lesioni in Ear pelle mouse per intravitale Multiphoton Imaging delle risposte immunitarie

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

Valutazione della riserva del flusso coronario dopo la miaocardia Ischemia Reperfusione nei ratti

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Un modello di ratto preclinico per lo studio delle lesioni ischemia-reperfusione in microchirurgia ricostruttiva

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Consegna intramiocardica ritardata di cellule staminali dopo l'infortunio di ricottamento ischemia in un modello Murine

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Lesione da ischemia-riperfusione dell'organo simulando cambiamenti emodinamici nel modello di trapianto di fegato di ratto

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...