Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

この作品は、SU Eisenhardtに &quot;ドイツ学術振興&quot;（EI 866/1-1）の助成金によって支えられている。

1。麻酔および監視<ol><li>動物実験を行う前に、適切な国や機関の倫理が場所でなければなりません。 180グラム - 120から体重の倫理委員会麻酔雄Sprague Dawleyラットからの承認に続く。イソフルラン気化器を介してプレキシグラスボックスに3体積％イソフルランおよびラットの内側に置く - 2を提供します。 </li><li>麻酔の適切なレベルが達成されるとすぐに（つま先またはテールピンチへの?...

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白血球-内皮相互作用、活性酸素種の産生および補体系の活性化IRI-誘発組織障害の主要な特徴である26は、罹患組織の微小循環は、炎症の発症の積分サイトと見なされます。別にそのようなフローチャンバーアッセイとしてex vivoでの実験を27日、28日からは、さらにin vivoでの関連で評価するために生体内イメージングの定評のあるモデルを提供するために?...

<table border="1"><tbody><tr><td> 機器の名前： </td><td> 会社名： </td><td> カタログ番号： </td><td> コメント： </td></tr><tr><td> Forene 100％（V / V） </td><td>アボット</td><td> B506 </td><td>イソフルランAPI </td></tr><tr><td> Terylene縫合</td><td> Serag Weissner </td><td> OC108000 </td><td></td></tr><tr><td> Portexファインボアポリエチレンチューブ</td><td>医療スミス</td><td> 800/100/100 </td><td> 0.28ミリメートル、内径</td></tr><tr><td> 0.9％食塩水</td><td> Fresinusカービ</td><td> 808771 </td><td></td></tr><tr><td>チェンジ·先端デラックス焼灼キット</td><td> Bovie医療</td><td> DEL1 </td><td></td></tr><tr><td> Abbocath-T 14G </td><td> Venisystems </td><td> G713- A01 </td><td>鏡筒として使用</td></tr><tr><td>サーボ人工呼吸器900C </td><td> Maquet </td><td></td><td>動物ventialtorとして使用</td></tr><tr><td>論理的な圧力トランスデューサー</td><td>医療スミス</td><td> MX1960 </td><td></td></tr><tr><td> Sirecust 404モニタ</td><td>ジーメンス</td><td></td><td></td></tr><tr><td> ABL 700ベンチトップ·アナライザ</td><td>放射計</td><td></td><td>血液ガス測定用</td></tr><tr><td>加温パッド</td><td> Effenberger </td><td> 8319 </td><td></td></tr><tr><td>アルミステージ</td><td> Alfun </td><td> AW7022 </td><td></td></tr><tr><td>手術用顕微鏡OPMI 6 SDFC </td><td>カールツァイス</td><td></td><td></td></tr><tr><td>顕微楽器ラボのら</td><td> S＆T </td><td> 767 </td><td></td></tr><tr><td> Biemer血管クリップ</td><td>ディーナー</td><td> 64.562 </td><td></td></tr><tr><td>鉗子を適用する</td><td>ディーナー</td><td> 64.568 </td><td> Biemer容器クリップの</td></tr><tr><td>ローダミン6G </td><td> Sigma-Aldrich社</td><td> R4127 </td><td></td></tr><tr><td>ワセリン白DAB </td><td>ウィンスロップ</td><td> 2726853 </td><td></td></tr><tr><td>メガネをカバー</td><td></td><td></td><td> 32×32ミリメートル</td></tr><tr><td></td><td></td><td></td><td> 生体内のセットアップ </td></tr><tr><td>ツァイスAxioはスコープ1 MAT </td><td>カールツァイス</td><td> 490036 </td><td>落射蛍光顕微鏡</td></tr><tr><td> 470nmのLEDが</td><td>カールツァイス</td><td> 423052 </td><td>蛍光光源</td></tr><tr><td>コリブリ2システム</td><td>カールツァイス</td><td> 423052 </td><td></td></tr><tr><td> Wプラン·アポクロマート20倍/ 1,0 DIC </td><td>カールツァイス</td><td> 421452 </td><td>水浸対物レンズ</td></tr><tr><td> AxioCam MRM牧師3つのFireWire </td><td>カールツァイス</td><td> 426509 </td><td>高解像度デジタルカメラ</td></tr><tr><td>アクシオビジョンLEソフトウェア</td><td>カールツァイス</td><td> 410130 </td><td>オフライン解析に使用</td></tr></tbody></table>

real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

虚血再灌流障害（IRI）は、このような脳卒中、心筋梗塞、腸管虚血などの移植や心臓血管手術後などの病的状態の大きな配列に関与しています。以前は虚血組織の1再灌流、不可逆的な予防のために不可欠ながら、組織損傷は、罹患組織の過度の炎症を誘発する。活性酸素種の産生、補体系の活性化と増加した血管透過性に隣接し、白血球の活性化は、再灌流時の炎症性組織損傷の病理学的カスケードの原則俳優の一人です。2、3、白血球の活性化は成る多段階のプロセスです。ローリング、強固な接着と輪廻のような補因子、ケモカイン、または血小板活性化因子としての化学誘引物質に応答して、接着分子との間の複雑な相互作用によって媒介される。4 &lt;Pクラスは= &quot;jove_content&quot;&gt;毛細血管細静脈における白血球のローリングは、主に彼らのカウンターリガンドとセレクチン5の相互作用によって媒介されていますが、内皮への白血球の強固な接着は細胞間接着分子（ICAM）、血管の細胞への結合を介してセレクチン制御です。接着分子（VCAM）。6、7 白血球-内皮相互作用の in vivo観察のためのゴールドスタンダードは、1968年に記載され生体内顕微鏡の技術である8。 IRI（虚血再灌流障害）の様々なモデルが様々な器官のために記載されているものの、9月12日わずか数は、画像品質の高いレベルでの微小血管床における白血球動員の直接可視化に適しています。8 ここでは精巣挙筋微小循環の後毛細血管細静脈のデジタル生体蛍光顕微鏡を促進するラット13の定性的かつ定量的に横紋筋組織中のIRI-研究のための白血球動員を分析し、技術を達成するための詳細なマニュアルを提供する便利な方法として。我々はさらに一般的な落とし穴を説明し、読者が本当に感謝することができ、かつ安全にする方法を実行する必要があり有用なヒントを提供しています。 ステップのプロトコル·バイ·ステップでは、より長い期間の動物はしっかりと麻酔を維持するのに十分な監視の下で呼吸制御された麻酔の使用を開始する方法を表しています。その後、卓越した光学分割のための薄い平らなシートとして挙準備を説明し、よく私たちの研究室で確立されているIRIで白血球イメージングのためのプロトコルを提供しています。

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

精巣挙筋微小循環における毛細血管細静脈のデジタル生体蛍光顕微鏡では、白血球 - 内皮相互作用への洞察を得るために便利な方法です。<emin vivoでの&gt;</em&gt;横紋筋組織の虚血再灌流障害（IRI）であった。ここでは、安全技術を実行し、その応用と限界を議論するための詳細なプロトコルを提供しています。

ラットの精巣挙筋の虚血再灌流障害における白血球 - 内皮相互作用のリアルタイムデジタルイメージング（IRI）

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

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In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

筋皮虚血再灌流傷害のラットモデル： 現場横腹直筋皮フラップで

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Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

マウスにおける急性腎障害とポスト傷害線維の虚血再灌流モデル

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

左前下の動脈の結紮を通して心筋虚血再灌流傷害のマウスモデル

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

ランゲンドルフ灌流ラット心臓における虚血再灌流傷害の誘導と評価

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

腸管虚血 - 再灌流傷害のマウスモデル

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

免疫応答の生体内多光子イメージングのためのマウスの耳の皮膚に虚血再灌流傷害を誘導します

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

ラットにおける心筋虚血再灌流後の冠動脈流量の評価

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

再建微小外科における虚血再灌流損傷の研究のための前臨床ラットモデル

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

マウスモデルにおける虚血再灌流損傷後の幹細胞の筋内内出産の遅延

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

ラット肝移植モデルにおける血球力学的変化をシミュレートすることによる臓器虚血再灌流損傷

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...