Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

이 작품은 SU Eisenhardt로 &quot;도이치 Forschungsgemeinschaft&quot;(EI 866/1-1)의 교부금에 의해 지원되었다.

1. 마취 및 모니터링 <ol><li> 동물 실험을 수행하기 전에 해당 국가 및 기관의 윤리는 제자리에 있어야합니다. 180g - 120에서 체중과 윤리위원회 마취 수컷 Sprague Dawley 쥐처럼의 승인을 따라. isoflurane 기화기를 통해 플렉시 글라스 상자에 3 시리즈의 % isoflurane과 쥐의 내부 배치 - 2을 제공합니다. </li><li> 마자 마취의 적절한 수준이 달성되면 (발가락이나 꼬리 공략하기 위해 반응의 부족) 쥐가 가중치?...

<ol>
	<li>Cetin, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+fucoidin+(a+neutrophil+rolling+inhibitor)+on+ischemia+reperfusion+injury:+experimental+study+in+rat+epigastric+island+flaps.">Protective effect of fucoidin (a neutrophil rolling inhibitor) on ischemia reperfusion injury: experimental study in rat epigastric island flaps.</a> Ann. Plast. Surg. 47, 540-546 (2001).</li><li>Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+xanthine+oxidase+and+granulocytes+in+ischemia-reperfusion+injury.">Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury.</a> Am. J. Physiol. 255, H1269-H1275 (1988).</li><li>Lazarus, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+mast+cells+in+ischaemia-reperfusion+injury+in+murine+skeletal+muscle.">The role of mast cells in ischaemia-reperfusion injury in murine skeletal muscle.</a> J Pathol. 191, 443-448 (2000).</li><li>van den Heuvel, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Review:+Ischaemia-reperfusion+injury+in+flap+surgery.">Review: Ischaemia-reperfusion injury in flap surgery.</a> J. Plast. Reconstr. Aesthet. Surg. 62, 721-726 (2009).</li><li>Rosen, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+surface+lectins+in+the+immune+system.">Cell surface lectins in the immune system.</a> Semin. Immunol. 5, 237-247 (1993).</li><li>van der Flier, A., Sonnenberg, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Function+and+interactions+of+integrins.">Function and interactions of integrins.</a> Cell Tissue Res. 305, 285-298 (2001).</li><li>Panes, J., Perry, M., Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Leukocyte-endothelial+cell+adhesion:+avenues+for+therapeutic+intervention.">Leukocyte-endothelial cell adhesion: avenues for therapeutic intervention.</a> Br. J. Pharmacol. 126, 537-550 (1999).</li><li>Gavins, F. N., Chatterjee, B. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravital+microscopy+for+the+study+of+mouse+microcirculation+in+anti-inflammatory+drug+research:+focus+on+the+mesentery+and+cremaster+preparations.">Intravital microscopy for the study of mouse microcirculation in anti-inflammatory drug research: focus on the mesentery and cremaster preparations.</a> J. Pharmacol. Toxicol. Methods. 49, 1-14 (2004).</li><li>Sutton, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Injury+of+the+renal+microvascular+endothelium+alters+barrier+function+after+ischemia.">Injury of the renal microvascular endothelium alters barrier function after ischemia.</a> Am. J. Physiol. Renal. Physiol. 285, 191-198 (2003).</li><li>Serracino-Inglott, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+nitric+oxide+synthase+expression+during+hepatic+ischemia-reperfusion.">Differential nitric oxide synthase expression during hepatic ischemia-reperfusion.</a> Am. J. Surg. 185, 589-595 (2003).</li><li>Eppinger, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mediators+of+ischemia-reperfusion+injury+of+rat+lung.">Mediators of ischemia-reperfusion injury of rat lung.</a> Am J Pathol. 150, 1773-1784 (1997).</li><li>Dumont, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+imaging+of+apoptotic+cell-membrane+changes+at+the+single-cell+level+in+the+beating+murine+heart.">Real-time imaging of apoptotic cell-membrane changes at the single-cell level in the beating murine heart.</a> Nat Med. 7, 1352-1355 (2001).</li><li>Baez, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+open+cremaster+muscle+preparation+for+the+study+of+blood+vessels+by+in+vivo+microscopy.">An open cremaster muscle preparation for the study of blood vessels by in vivo microscopy.</a> Microvasc Res. 5, 384-394 (1973).</li><li>Woeste, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Octreotide+attenuates+impaired+microcirculation+in+postischemic+pancreatitis+when+administered+before+induction+of+ischemia.">Octreotide attenuates impaired microcirculation in postischemic pancreatitis when administered before induction of ischemia.</a> Transplantation. 86, 961-967 (2008).</li><li>Schultz, J. E., Hsu, A. K., Gross, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphine+mimics+the+cardioprotective+effect+of+ischemic+preconditioning+via+a+glibenclamide-sensitive+mechanism+in+the+rat+heart.">Morphine mimics the cardioprotective effect of ischemic preconditioning via a glibenclamide-sensitive mechanism in the rat heart.</a> Circ. Res. 78, 1100-1104 (1996).</li><li>Dobschuetz, E. v. o. n. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+intravital+fluorescence+microscopy--a+novel+method+for+the+assessment+of+microvascular+permeability+in+acute+pancreatitis.">Dynamic intravital fluorescence microscopy--a novel method for the assessment of microvascular permeability in acute pancreatitis.</a> Microvasc Res. 67, 55-63 (2004).</li><li>Vutskits, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adverse+effects+of+methylene+blue+on+the+central+nervous+system.">Adverse effects of methylene blue on the central nervous system.</a> Anesthesiology. 108, 684-692 (2008).</li><li>Takasu, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+survival+time+with+combined+early+blood+transfusion+and+fluid+administration+in+uncontrolled+hemorrhagic+shock+in+rats.">Improved survival time with combined early blood transfusion and fluid administration in uncontrolled hemorrhagic shock in rats.</a> J. Trauma. 8, 312-316 (2010).</li><li>Proctor, K. G., Busija, D. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationships+among+arteriolar,+regional,+and+whole+organ+blood+flow+in+cremaster+muscle.">Relationships among arteriolar, regional, and whole organ blood flow in cremaster muscle.</a> Am. J. Physiol. 249, 34-41 (1985).</li><li>Bagher, P., Segal, S. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Mouse+Cremaster+Muscle+Preparation+for+Intravital+Imaging+of+the+Microcirculation.">The Mouse Cremaster Muscle Preparation for Intravital Imaging of the Microcirculation.</a> J. Vis. Exp. (52), e2874 (2011).</li><li>Kanwar, S., Hickey, M. J., Kubes, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Postischemic+inflammation:+a+role+for+mast+cells+in+intestine+but+not+in+skeletal+muscle.">Postischemic inflammation: a role for mast cells in intestine but not in skeletal muscle.</a> Am. J. Physiol. 275, 212-218 (1998).</li><li>Leoni, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflamed+phenotype+of+the+mesenteric+microcirculation+of+melanocortin+type+3+receptor-null+mice+after+ischemia-reperfusion.">Inflamed phenotype of the mesenteric microcirculation of melanocortin type 3 receptor-null mice after ischemia-reperfusion.</a> FASEB J. 22, 4228-4238 (2008).</li><li>Simoncini, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interaction+of+oestrogen+receptor+with+the+regulatory+subunit+of+phosphatidylinositol-3-OH+kinase.">Interaction of oestrogen receptor with the regulatory subunit of phosphatidylinositol-3-OH kinase.</a> Nature. 407, 538-541 (2000).</li><li>Woollard, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathophysiological+levels+of+soluble+P-selectin+mediate+adhesion+of+leukocytes+to+the+endothelium+through+Mac-1+activation.">Pathophysiological levels of soluble P-selectin mediate adhesion of leukocytes to the endothelium through Mac-1 activation.</a> Circ. Res. 103, 1128-1138 (2008).</li><li>Mori, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ischemia-reperfusion+induced+microvascular+responses+in+LDL-receptor+-/-+mice.">Ischemia-reperfusion induced microvascular responses in LDL-receptor -/- mice.</a> Am. J. Physiol. 276, H1647-H1654 (1999).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+Molecular+Changes+Induced+by+Ischemia/Reperfusion+in+Human+Free+Muscle+Flap+Tissue+Samples.">Monitoring Molecular Changes Induced by Ischemia/Reperfusion in Human Free Muscle Flap Tissue Samples.</a> Ann. Plast. Surg. , (2011).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+activation-specific+human+anti-{alpha}M{beta}2+single-chain+antibodies+as+potential+diagnostic+tools+and+therapeutic+agents.">Generation of activation-specific human anti-{alpha}M{beta}2 single-chain antibodies as potential diagnostic tools and therapeutic agents.</a> Blood. 109, 3521-3528 (2007).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissociation+of+pentameric+to+monomeric+C-reactive+protein+on+activated+platelets+localizes+inflammation+to+atherosclerotic+plaques.">Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.</a> Circ Res. 105, 128-137 (2009).</li><li>Eisenhardt, S. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=C-reactive+protein:+how+conformational+changes+influence+inflammatory+properties.">C-reactive protein: how conformational changes influence inflammatory properties.</a> Cell Cycle. 8, 3885-3892 (2009).</li><li>Granger, D. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Physiology and pathophysiology of leukocyte adhesion. , 520 (1995).</li><li>Baatz, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Kinetics+of+white+blood+cell+staining+by+intravascular+administration+of+rhodamine+6G.">Kinetics of white blood cell staining by intravascular administration of rhodamine 6G.</a> Int. J. Microcirc. Clin. Exp. 15, 85-91 (1995).</li><li>Mempel, T. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+imaging+of+leukocyte+trafficking+in+blood+vessels+and+tissues.">In vivo imaging of leukocyte trafficking in blood vessels and tissues.</a> Curr. Opin. Immunol. 16, 406-417 (2004).</li><li>Abbitt, K. B., Rainger, G. E., Nash, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+fluorescent+dyes+on+selectin+and+integrin-mediated+stages+of+adhesion+and+migration+of+flowing+leukocytes.">Effects of fluorescent dyes on selectin and integrin-mediated stages of adhesion and migration of flowing leukocytes.</a> J. Immunol. Methods. 239, 109-119 (2000).</li></ol>

백혈구 - 내피 상호 작용, 반응성 산소 종의 생산과 보완 시스템의 활성화 IRI 유발 조직의 기능 장애의 주요 기능입니다. 영향을받는 조직 중 26 microcirculation가 염증 발병에 대한 통합 사이트로 간주됩니다. 이외에도 이러한 흐름 챔버 assays 27, 28 등 전직 생체내 실험에서 더 나아가 생체내 관련에서 평가하는 intravital 이미징 잘 창업 모델을 제공할 의무이다. IRI는 여러...

<table border="1"><tbody><tr><td> 장비의 명칭 : </td><td> 회사명 : (주) </td><td> 카탈로그 번호 : </td><td> 의견 : </td></tr><tr><td> Forene 백퍼센트 (V / V) </td><td> 대수도 원장 </td><td> B506 </td><td> API isoflurane </td></tr><tr><td> Terylene 봉합사 </td><td> Serag Weissner </td><td> OC108000 </td><td></td></tr><tr><td> Portex 파인 Polythene 튜빙을 내경 </td><td> 의료 스미스 (Smiths) </td><td> 800/100/100 </td><td> 0.28 mm 내경 </td></tr><tr><td> 0,9 % 생리 식염수 </td><td> Fresinus Kabi </td><td> 808,771 </td><td></td></tr><tr><td> 변경 - 어 - 팁 디럭스 소작 키트 </td><td> Bovie 의료 </td><td> DEL1 </td><td></td></tr><tr><td> Abbocath-T 14G </td><td> Venisystems </td><td> G713- A01 </td><td> 렌즈 튜브로 사용 </td></tr><tr><td> 서보 환풍기 900C </td><td> Maquet </td><td></td><td> 동물 ventialtor로 사용 </td></tr><tr><td> 논리 압력 변환기 </td><td> 의료 스미스 (Smiths) </td><td> MX1960 </td><td></td></tr><tr><td> Sirecust 404 모니터 </td><td> 지멘스 </td><td></td><td></td></tr><tr><td> ABL 700 Benchtop 분석기 </td><td> 라디오 미터 </td><td></td><td> 혈액 가스 측정을위한 </td></tr><tr><td> 생리대 </td><td> Effenberger </td><td> 8319 </td><td></td></tr><tr><td> 알루미늄 무대 </td><td> Alfun </td><td> AW7022 </td><td></td></tr><tr><td> 외과 현미경 OPMI 6-SDFC </td><td> 칼 자이스 혈구 </td><td></td><td></td></tr><tr><td> Microsurgical 악기 연구소의동부 표준시 </td><td> S &amp; T </td><td> 767 </td><td></td></tr><tr><td> Biemer 용기 클립 </td><td> Diener </td><td> 64.562 </td><td></td></tr><tr><td> 포셉 적용 </td><td> Diener </td><td> 64.568 </td><td> Biemer 용기 클립을위한 </td></tr><tr><td> Rhodamine 6G </td><td> 시그마 - 올드 리치 </td><td> R4127 </td><td></td></tr><tr><td> 바셀린 하얀 DAB </td><td> Winthrop </td><td> 2,726,853 </td><td></td></tr><tr><td> 안경을 커버 </td><td></td><td></td><td> 32x32 mm </td></tr><tr><td></td><td></td><td></td><td> Intravital 설치 </td></tr><tr><td> Zeis Axio 범위-1 MAT </td><td> 칼 Zeis </td><td> 490,036 </td><td> epifluorescence 현미경 </td></tr><tr><td> 470 nm의은 LED가 </td><td> 칼 Zeis </td><td> 423,052 </td><td> 형광 광원 </td></tr><tr><td> Colibri 2 시스템 </td><td> 칼 Zeis </td><td> 423,052 </td><td></td></tr><tr><td> W 플랜 - 고차 색지움 20x / 1,0 DIC </td><td> 칼 Zeis </td><td> 421,452 </td><td> 물 침지 목표 </td></tr><tr><td> AxioCam MRM 목사 3 파이어 와이어 </td><td> 칼 Zeis </td><td> 426,509 </td><td> 고해상도 디지털 카메라 </td></tr><tr><td> Axio LE 비전 소프트웨어 </td><td> 칼 Zeis </td><td> 410,130 </td><td> 오프라인 분석을 위해 사용 </td></tr></tbody></table>

real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

돌이킬 방지에 필수적인 반면 국소 빈혈 - reperfusion 상해 (IRI)는 같은 대뇌 뇌졸중, 심근 경색, 장내 국소 빈혈뿐만 아니라 이전에 허혈성 조직 이식과 심장 혈관 수술 1. Reperfusion 다음과 같은 병적인 조건의 큰 배열에 연루되어 조직 부상이 영향을받는 조직의 과도한 염증을 elicits. 반응성 산소 종, 보완 시스템의 활성화의 생산 및 microvascular 투자율 증가 인접, leukocytes의 활성화가 reperfusion 동안 염증성 조직 손상의 병적인 폭포의 주요 배우 중 하나이다. 2, 3 백혈구 활성화 구성된 다단계 프로세스입니다 , 압연 확고한 접착력과 윤회와 같은 보완 요인, chemokines, 또는 혈소판 - 활성 요소로 chemoattractants에 대한 응답으로 유착 분자 사이의 복잡한 상호 작용에 의해 매개된다. 4 &lt;P 클래스 = &quot;jove_content&quot;&gt; postcapillary venules에서 백혈구 구름은 주로 자신의 카운터 리간드, 내피로 leukocytes의 확고한 유착 세포 접착 분자 (ICAM)과 혈관 세포에 바인딩을 통해 selectin 조절과 함께 selectins 5의 상호 작용에 의해 매개되는 동안 유착 분자 (VCAM). 6, 7 백혈구 - 내피 상호 작용의 생체내 관찰에 대한 골드 표준 먼저 1968 년 설명한 intravital 현미경의 기법이다 8. IRI (국소 빈혈 reperfusion-상해)의 다양한 모델이 여러 장기에 대한 설명되어 있지만, 9-12 오직 소수 이미지 품질을 높은 수준에서 microvascular 침대에 백혈구 모집의 직접적인 시각화에 적합 8. 우리는 여기서 cremasteric microcirculation에 postcapillary venule의 디지털 intravital epifluorescence 현미경을 촉진질적 및 양적 줄무늬가 근육 조직에서 IRI - 연구 백혈구 모집을 분석하고 기술을 달성을위한 상세한 매뉴얼을 제공하는 편리한 방법으로 쥐 13. 우리는 더 이상 일반적인 함정을 설명하며 독자가 진정으로 감사 있도록하고, 안전하게 방법을 수행해야합니다 유용한 팁을 제공합니다. 단계 프로토콜에 의해 단계에서 우리는 시간의 긴 기간 동안 동물이 단단히 anesthetized 유지하는 데 충분한 감시하에 호흡 조절 마취를 시작하는 방법을 묘사. 그러면 뛰어난 광학 해상도 얇은 평면 시트로 cremasteric 준비를 설명하고 잘 우리의 실험실에 설립되었습니다 IRI에서 백혈구 이미징을위한 프로토콜을 제공합니다.

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

cremasteric microcirculation의 postcapillary venules 디지털 intravital epifluorescence 현미경은 백혈구 - 내피 상호 작용에 대한 통찰력을 얻을 수있는 편리한 방법입니다<em&gt; 생체내에서</em&gt; 평형으로 뻗은 줄이있는 근육 조직의 국소 빈혈 reperfusion - 상해 (IRI) 인치 우리는 여기서 안전하게 기술을 수행하고 응​​용 프로그램과 한계를 논의하기 위해 상세한 프로토콜을 제공합니다.

쥐 Cremaster 근육의 국소 빈혈 reperfusion의 - 상해에서 백혈구 - 내피 상호 작용의 실시간 디지털 이미징 (IRI)

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

Myocutaneous 국소 빈혈 reperfusion 상해의 쥐 모델 : 현장 가로 직근 abdominis의 Myocutaneous 플랩의

Free tissue transfer is the gold standard of reconstructive surgery to repair complex defects not amenable to local options or those requiring composite ...

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Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

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The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

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Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

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면역 반응의 생체 내에 다중 광자 이미징을위한 마우스 귀 피부에 유도 허혈 - 재관류 손상

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

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쥐의 심근 허혈 재관류 후 관상 동맥 흐름 예비의 평가

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

재건 미세 수술에서 허혈 재관류 손상의 연구를위한 전 임상 쥐 모델

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

뮤린 모델에서 허혈혈 재관류 부상 후 줄기 세포의 지연 된 자궁 내 납품

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

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쥐 간 이식 모형에 있는 혈역학적인 변경을 시뮬레이션하여 기관 허혈-재관류 상해

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...