Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Dette arbeidet ble støttet av et stipend av &quot;Deutsche Forschungsgemeinschaft&quot; til SU Eisenhardt (EI 866/1-1).

1. Anestesi og overvåking <ol><li> Egnede nasjonale og institusjonelle etikk bør være på plass før du utfører dyreforsøk. Etter godkjenning fra etisk komité anesthetize mannlige Sprague Dawley rotter med en kroppsvekt 120-180 gram. Lever 2-3 vol% isofluran til en pleksiglass boks via isofluran vaporizer og plassere rotte inni. </li><li> Så snart det riktige nivået av anestesi oppnås (mangel på reaksjon til tå eller hale knipe) rotta vektes og barbert på ventrale cervical området. </li><li> Plasser rotte...

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Leukocytt-endotelial interaksjon, produksjon av reaktive oksygenforbindelser og aktivering av komplementsystemet er de viktigste funksjonene i IRI-indusert vev dysfunksjon. 26 mikrosirkulasjonen i det berørte vevet er regnet som den integrerende nettstedet for inflammatorisk utbruddet. Bortsett fra ex vivo eksperimenter som flyt kammerkor analyser 27, 28 er det obligatorisk å gi veletablerte modeller av intravital bildebehandling for å ytterligere evaluere in vivo relevans. Sel...

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real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

Iskemi-reperfusjon skade (IRI) har vært innblandet i et stort utvalg av patologiske tilstander som hjerneslag, hjerteinfarkt, intestinal iskemi samt følge transplantasjon og kardiovaskulær kirurgi. En reperfusjon av tidligere iskemisk vev, mens avgjørende for forebygging av irreversibel vevsskade, utløser overdreven betennelse i vev. I tilknytning til produksjon av reaktive oksygenforbindelser, aktivering av komplementsystemet og økt mikrovaskulær permeabilitet, er aktivering av leukocytter en av de fremste aktørene i patologisk kaskade av inflammatoriske vevsskader under reperfusjon. 2, 3 leukocyttantigener aktivisering en multistep prosess bestående av 4 rullende, fast heft og sjelevandring og formidles av et komplekst samspill mellom adhesjonsmolekyler i respons til chemoattractants som komplement faktorer, chemokines eller blodplate-aktiverende faktor. &lt;p class = &quot;jove_content&quot;&gt; Mens leukocytt rullende i postcapillary venules hovedsakelig skjer av et samspill av selectins 5 med sine telleren ligander, fast adhesjon av leukocytter til endotel er selektin-kontrollert via binding til intercellulær adhesjonsmolekyler (ICAM) og vaskulær cellulære adhesjonsmolekyler (VCAM). 6, 7 Gullstandard for in vivo observasjon av leukocytt-endotelial interaksjon er teknikken for intravital mikroskopi, først beskrevet i 1968. 8 Selv om ulike modeller for IRI (iskemi-reperfusjon skade) har blitt beskrevet for ulike organer, 9-12 bare få er egnet for direkte visualisering av leukocytt rekruttering i mikrovaskulær sengen på et høyt nivå av bildekvalitet. 8 Vi her fremme digital intravital epifluorescence mikroskopi av postcapillary venule i cremasteric mikrosirkulasjonenav rotte 13 som en praktisk metode for å kvalitativt og kvantitativt analysere leukocytt rekruttering for IRI-forskning i tverrstripet muskelvev og gir en detaljert manual for å utføre teknikken. Vi ytterligere illustrere vanlige fallgruvene og gir nyttige tips som bør sette leseren virkelig setter pris på, og sikkert utføre metoden. I en trinnvis protokollen skildre hvordan vi skal komme i gang med åndedrett kontrollert anestesi henhold tilstrekkelig overvåking for å holde dyret fast bedøvet for lengre perioder av gangen. Så beskriver cremasteric forberedelse som en tynn flat ark for fremragende optisk oppløsning og gi en protokoll for leukocytt bildebehandling i IRI som er godt etablert i våre laboratorier.

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

Digital intravital epifluorescence mikroskopi av postcapillary venules i cremasteric mikrosirkulasjonen er en praktisk metode for å få innsikt i leukocytt-endotelial interaksjon<em&gt; In vivo</em&gt; I iskemi-reperfusjon skade (IRI) av tverrstripet muskelvev. Vi her gi en detaljert protokoll trygt utføre teknikken og diskutere sine applikasjoner og begrensninger.

Real-time Digital Imaging av leukocyttantigener-endotelial Interaksjon i iskemi-reperfusjon Injury (IRI) av Rat Cremaster Muscle

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

In situ Tverrgående rectus abdominis myocutaneous Flap: A Rat Modell av myocutaneous Ischemia reperfusjonsskade

Free tissue transfer is the gold standard of reconstructive surgery to repair complex defects not amenable to local options or those requiring composite ...

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Iskemi-reperfusjon Modell av akutt nyreskade og Post Injury fibrose i Mus

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

En murine modell av myokardiskemi-reperfusjon Injury gjennom Ligation av Left Anterior Synkende Artery

Acute or chronic myocardial infarction (MI) are cardiovascular events resulting in high morbidity and mortality. Establishing the pathological mechanisms ...

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The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

Murine modell av intestinal iskemi-reperfusjon Injury

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Induserende iskemi-reperfusjon Injury i Mouse Ear Skin for intra multiphoton avbildning av immunrespons

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

Evaluering av koronar Flow Reserve etter hjerteinfarkt ischemia reperfusion i rotter

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

En pre-klinisk rotte modell for studier av ischemia-reperfusion skade i rekonstruktiv mikrokirurgi

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Forsinket intramyokarell levering av stamceller etter iskemi reperfusjonsskade i en Murine-modell

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Organ iskemi-reperfusjon skade ved å simulere hemoodynamiske endringer i rotte levertransplantasjon modell

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...