Name: real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle
Uploaded: 2012-08-05T14:00:00
Description: Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Bu çalışma SU Eisenhardt için &quot;Deutsche Forschungsgemeinschaft&quot; (EI 866/1-1) bir hibe ile desteklenmiştir.

1. Anestezi ve İzleme <ol><li> Hayvan deneylerinde gerçekleştirmeden önce uygun ulusal ve kurumsal etik yerde olmalıdır. 180 gr - 120 bir vücut ağırlığı ile etik komite uyutmak erkek Sprague Dawley sıçan onayını takiben. Izofluran vaporizer üzeri pleksiglas kutu 3 vol% izofluran ve sıçan içine yerleştirin - 2 sunun. </li><li> En kısa sürede anestezi uygun düzeyde sağlanır sağlanmaz (parmak veya kuyruk tutam tepki eksikliği) sıçan ağırlıklandırılmış ve ventral servikal alanda traş ...

<ol>
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Lökosit-endotel etkileşimi, reaktif oksijen türlerinin üretimi ve kompleman sisteminin aktivasyonu IRI bağlı doku disfonksiyonunun temel özellikleridir. Etkilenen doku 26 mikrosirkülasyon inflamatuar başlangıcı için ayrılmaz yeri olarak kabul edilir. Dışında bu akış odası deneyleri 27, 28, ex vivo deneyler daha da in vivo alaka içinde değerlendirmek intravital görüntüleme köklü modelleri sağlamak zorunludur. IRI çeşitli organ sistemlerine suç...

<table border="1"><tbody><tr><td> Ekipman Adı: </td><td> Şirket: </td><td> Katalog No: </td><td> Yorumlar: </td></tr><tr><td> Forene% 100 (V / V) </td><td> Başrahip </td><td> B506 </td><td> API izofluran </td></tr><tr><td> Terylen Sütür </td><td> Serag Weissner </td><td> OC108000 </td><td></td></tr><tr><td> Portex Güzel Polietilen Boru Bore </td><td> Tıp Smiths </td><td> 800/100/100 </td><td> 0.28 mm iç çapı </td></tr><tr><td> % 0,9 tuz çözeltisi </td><td> Fresinus Kabi </td><td> 808771 </td><td></td></tr><tr><td> Değişim-A-ucu deluxe koter kiti </td><td> Bovie Tıp </td><td> DEL1 </td><td></td></tr><tr><td> Abbocath-T 14G </td><td> Venisystems </td><td> G713- A01 </td><td> objektif tüpü olarak kullanılan </td></tr><tr><td> Servo Vantilatör 900C </td><td> Maquet </td><td></td><td> hayvan ventialtor olarak kullanılan </td></tr><tr><td> Mantıksal basınç dönüştürücü </td><td> Tıp Smiths </td><td> MX1960 </td><td></td></tr><tr><td> Sirecust 404 Monitör </td><td> Siemens </td><td></td><td></td></tr><tr><td> ABL 700 Masaüstü Analyzer </td><td> Radyometre </td><td></td><td> kan gazı ölçümü için </td></tr><tr><td> Isıtma pedi </td><td> Effenberger </td><td> 8319 </td><td></td></tr><tr><td> Alüminyum sahne </td><td> Alfun </td><td> AW7022 </td><td></td></tr><tr><td> Cerrahi mikroskop OPMI 6-SDFC </td><td> Carl Zeiss </td><td></td><td></td></tr><tr><td> Mikro cerrahi aletlerin laboratuvar set </td><td> S &amp; T </td><td> 767 </td><td></td></tr><tr><td> Biemer damar klip </td><td> Diener </td><td> 64.562 </td><td></td></tr><tr><td> Forseps uygulanması </td><td> Diener </td><td> 64.568 </td><td> Biemer damar klip için </td></tr><tr><td> Rodamin 6G </td><td> Sigma-Aldrich </td><td> R4127 </td><td></td></tr><tr><td> Vazelin beyaz DAB </td><td> Winthrop </td><td> 2726853 </td><td></td></tr><tr><td> Gözlük Kapak </td><td></td><td></td><td> 32x32 mm </td></tr><tr><td></td><td></td><td></td><td> Intravital kurulum </td></tr><tr><td> Zeiss Axio Kapsam A-1 MAT </td><td> Carl Zeiss </td><td> 490036 </td><td> Epifloresans mikroskop </td></tr><tr><td> 470 nm LED </td><td> Carl Zeiss </td><td> 423052 </td><td> floresan ışık kaynağı </td></tr><tr><td> Colibri 2 Sistem </td><td> Carl Zeiss </td><td> 423052 </td><td></td></tr><tr><td> B Planı-Apochromat 20x / 1,0 DIC </td><td> Carl Zeiss </td><td> 421452 </td><td> suya daldırma hedefi </td></tr><tr><td> AxioCam MRM Rev 3 FireWire </td><td> Carl Zeiss </td><td> 426509 </td><td> yüksek çözünürlüklü dijital kamera </td></tr><tr><td> Axio vizyon LE yazılımı </td><td> Carl Zeiss </td><td> 410130 </td><td> çevrimdışı analiz için kullanmak </td></tr></tbody></table>

real-time digital imaging of leukocyte-endothelial interaction in ischemia-reperfusion injury (iri) of the rat cremaster muscle

Tersinmez önlenmesi için gerekli süre işemi-reperfüzyon yaralanması (IRI), serebral inme, miyokard enfarktüsü, bağırsak iskemi yanı sıra, daha önce iskemik doku transplantasyonu ve kardiyovasküler cerrahi. 1 Reperfüzyon aşağıdaki gibi patolojik durumların büyük bir dizi rolü de ortaya konmuştur doku hasarı, etkilenen doku aşırı enflamasyon ortaya çıkarır. Reaktif oksijen türlerinin, tamamlayıcı sisteminin aktivasyonu üretimine ve mikrovasküler geçirgenliğin artış bitişik, lökositlerin aktivasyonu reperfüzyon esnasında inflamatuar doku hasarının patolojik basamağında prensibi oyuncular biridir. 2, 3 Leukocyte aktivasyonu kapsayan çok aşamalı bir işlemdir , haddeleme firma adezyon ve göçü ve bu tür tamamlayıcı faktörleri, kemokinleri veya trombosit aktive edici faktör olarak chemoattractants yanıt olarak adezyon molekülleri arasındaki karmaşık bir etkileşim aracılık eder. 4 &lt;p class = &quot;jove_content&quot;&gt; postkapiller venlerde lökosit yuvarlanmasını ağırlıklı olarak kendi sayacı ligandlar, endotele lökositlerin sıkı adezyon adezyon molekülleri (ICAM) ve vasküler hücresel bağlama yoluyla selektin-kontrollü ile selektinler 5 etkileşimi aracılık ederken yapışma molekülleri (VCAM). 6, 7 Lökosit-endotel etkileşimi in vivo gözlem için altın standart olarak ilk kez 1968 yılında tanımlanan intravital mikroskopi tekniğidir. 8 IRI (iskemi-reperfüzyon) çeşitli modelleri çeşitli organları için tarif edilmiş olsa da, 9-12 sadece birkaç görüntü kalitesi yüksek bir seviyede mikrovasküler yatakta lökosit göçünün doğrudan görüntülenmesi için uygundur. 8 Biz burada kremasterik mikrosirkülasyonda postkapiller venül sayısal intravital Epifloresans mikroskopi teşvikkalitatif ve kantitatif olarak çizgili kas dokusunda IRI-araştırma için lökosit göçünün analiz ve tekniği gerçekleştirmek için ayrıntılı bir el kitabı sunmak için uygun bir yöntem olarak sıçan 13. Biz daha fazla ortak tuzaklar göstermek ve okuyucu gerçekten takdir sağlamak ve güvenli bir yöntem yapmalıdır yararlı ipuçları sunmaktadır. Adım protokol tarafından bir adımda biz daha uzun süre için hayvan sıkıca anestezi altında tutmak için yeterli gözetim altında solunum kontrollü anestezi ile başlamak nasıl betimliyor. Daha sonra üstün optik çözünürlük için bir ince düz bir levha olarak kremasterik hazırlık tanımlamak ve iyi laboratuarımızda kurulmuştur IRI lökosit görüntüleme için bir protokol sağlar.

Watch this Scientific Journal Video about Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle at JoVE.com

Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury IRI of the Rat Cremaster Muscle

Kremasterik mikrosirkülasyonda postkapiller venüllerde Dijital intravital Epifloresans mikroskopi lökosit-endotel etkileşimi içgörü kazanmak için uygun bir yöntemdir<em&gt; In vivo</em&gt; Çizgili kas dokusunda iskemi-reperfüzyon (IRI) içinde. Biz burada güvenle tekniği gerçekleştirmek ve uygulamaları ve sınırlamaları tartışmak için ayrıntılı bir protokol sağlar.

Sıçan Cremaster&#39;ın kas iskemi-reperfüzyon Hasarı içinde Lökosit-endotelyal Etkileşim Gerçek zamanlı Dijital Görüntüleme (IRI)

Ischemia-reperfusion injury (IRI) has been implicated in a large array of pathological conditions such as cerebral stroke, myocardial infarction, ...

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Real-time Digital Imaging of Leukocyte-endothelial Interaction in Ischemia-reperfusion Injury (IRI) of the Rat Cremaster Muscle

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Bağırsak İskemi-reperfüzyon Hasarı Murin Modeli

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Bağışıklık Yanıtları intravital multiphoton Görüntüleme Fare Kulak Skin uyaran İskemi-reperfüzyon yaralanması

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Evaluation of Coronary Flow Reserve After Myocardial Ischemia Reperfusion in Rats

Farelerde miyokardiyal Iskemi reperfüzyon sonrası koroner akış rezervi değerlendirilmesi

Coronary artery disease is the leading cause of death worldwide. After an acute myocardial infarction, early and successful myocardial intervention via ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Rekonstrüktif Mikrocerrahide İskemi-Reperfüzyon Hasarı Nın İnceleştirilmesinde Klinik Öncesi Sıçan Modeli

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Bir Murine Modelinde İskemi Reperfüzyon Yaralanması Sonrası Kök Hücrelerin Gecikmiş İntramiyokardiyal Teslimi

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Sıçan Karaciğer Nakli Modelinde Hemodinamik Değişiklikleri Simüle Ederek Organ İskemi-Reperfüzyon Yaralanması

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...