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DOI :
10.3791/3982-v
May 1st, 2012
Chapters
0:05
Title
1:21
Sample Preparation: Preparing Cover Slips
2:27
Sample Preparation: Preparing Bacillus subtilis Cells
3:58
Sample Preparation: Preparing Saccharomyces cerevisiae Cells
5:03
Image Acquisition
9:44
Results: Visualization of Cortex Organization and Dynamics
13:41
Conclusion
총 내부 반사 형광 (TIRF) 현미경 가까이 높은 콘트라스트와 시간적 해상도의 세포 표면 구조를 관찰하기위한 강력한 방법입니다. 우리는 TIRF은 세포 벽 - 동봉된 세균과 곰팡이 세포의 피질에서 단백질 역학을 공부 고용 수있는 방법을 보여줍니다.
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