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DOI :
10.3791/3991-v
•
10:45 min
July 20th, 2012
Chapters
0:05
Title
1:33
Imaging Plate Preparation
2:29
Imaging Parameters for the Zeiss LSM 710 Confocal Microscope
3:37
Optimizing the Imaging Parameters
4:49
Mitochondrial-fusion Assay Set-up
7:36
Analyzing the PA-GFP Signal Intensity
8:17
Results: Representative Mitochondrial-fusion Assays
9:49
Conclusion
线粒体融合是衡量跟踪矩阵针对性photoconverted整个网络随着时间的推移线粒体的GFP平衡的。到目前为止,只有一个细胞可能受到的时间在一个小时长的动力学分析。我们提出了一个方法,同时测量多个细胞,从而加快了数据采集过程。
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