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DOI :
10.3791/3995-v
•
13:13 min
December 3rd, 2012
Chapters
0:05
Title
1:24
High Pressure Freezing
2:45
Freeze-substitution
4:27
Infiltration and Polymerization
6:15
PALM Imaging
8:37
SEM Imaging
9:29
Aligning PALM and EM Images
11:51
Representative Nano-fEM Images
12:39
Conclusion
本地化荧光标记的蛋白质在电子显微镜下,我们描述了一种方法。荧光是第一次使用光激活定位显微技术在超薄切片本地化。然后,这些图像被对齐到相同的部分的电子显微镜照片。
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