Name: visualization and analysis of blood flow and oxygen consumption in hepatic microcirculation: application to an acute hepatitis model
Uploaded: 2012-08-04T17:00:00
Description: Watch this Scientific Journal Video about Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model at JoVE.com

著者感谢沙大冢女士提供技术援助和帮助实验。部分支持这项研究是由教育部，科学，体育和文化活动，资助青年科学家（二），2010，22700476，Suzuken 2010年纪念基金会为KT这项研究是由研究和开发的支持援助下一代集成模拟生命物质的发展和使用的文部科学省的下一代超级计算机项目的一部分，在垂询的ERATO Suematsu气体生物学项目的一部分。

1。用异硫氰酸荧光素异构体（FITC标记）标记红细胞我们使用的所有实验协议，由庆应义塾大学动物医学院护理委员会的批准。 <ol><li>捐助国小鼠麻醉，做开腹手术切口后退出下腔静脉全血1毫升含有少量的肝素的注射器。巴比妥钠注射液（100毫克/公斤的IP），鼠标立即被安乐死。 </li><li>洗了两次全血离心5分钟400克摆动转头用PBS（pH值7.4）。 </li><li>溶解在100毫米的Na <...

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组织提供氧气，是微循环的重要作用，和很多文件描述微血管解剖和流变6有关的疾病。肝血流的动脉和静脉血液的组合。肝脏的血流量约30％是提供由门静脉，肝动脉和其他的70％提供的含氧血，脾和消化道7不佳的含氧的血液从静脉流出。肝脏疾病，如脂肪肝，休克，或肿瘤在肝脏微循环的血流量变化的。为了确定组织氧合，需要测量的微循环血流量和氧浓度。市售仪器的缺乏，...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>钯（II）meso-四（4 - 羧基苯基）卟啉</td><td>科学前沿，公司</td><td> PdT790 </td></tr><tr><td>异硫氰酸荧光素异构体的我</td><td> Aldrich公司</td><td> F7250 </td></tr><tr><td>对乙酰氨基酚</td><td> Aldrich公司</td><td> A7085 </td></tr></tbody></table> <table border="1"><tbody><tr><td> 设备名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td></td><td></td><td></td><td> 设备 </td></tr><tr><td>光电倍增管</td><td>滨松光子学株式会社</td><td> H10722-20 </td></tr></tbody></table>

visualization and analysis of blood flow and oxygen consumption in hepatic microcirculation: application to an acute hepatitis model

因为肝脏耗氧量是比较高的，但约有70％的肝脏血液供应不足含氧从胃肠道，脾的门静脉血液，在肝脏中的氧气供应和需求之间有一个相当大的差异。氧气从门静脉的终末支流入通过血窦中央小静脉血液运送到肝，这使得在肝小叶的氧气梯度。氧梯度是一个重要的物理参数，包括酶在肝脏微循环的上游和下游的表达，但在肝脏微循环的氧气消耗测量技术的缺乏，已经推迟了与氧气在肝脏代谢机制的澄清。因此，我们用FITC标记的红细胞，肝脏微循环的可视化和使用激光辅助磷光测量氧气在微血管氧分压。 noncontaCT和可以量化的连续光学测量血流速度，血管直径，有关肝脏的氧气消耗的氧气梯度。在急性肝炎模型中，我们通过管理对乙酰氨基酚对小鼠，我们发现增加了门户网站和中央静脉氧气压力，但在血窦减少氧梯度，表明肝细胞坏死在pericentral区域转移氧气压力和影响酶的表达在汇管区。总之，我们的光学测量肝血流动力学和氧消耗的方法可以揭示有关肝病的机制。

Watch this Scientific Journal Video about Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model at JoVE.com

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model

光学系统的开发，可视化与FITC标记的红细胞肝脏微循环和氧气在微血管局部压力测量激光辅助磷光。这种方法可以用来研究生理和病理机制，通过分析微血管结构，直径，血流速度和氧张力。

可视化和肝脏微循环血流量和耗氧量分析：急性肝炎模型中的应用

There is a considerable discrepancy between oxygen supply and demand in the liver because hepatic oxygen consumption is relatively high but about 70% of ...

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