पोलीमरेज़ चेन रिएक्शन: बेसिक प्रोटोकॉल प्लस समस्या निवारण और अनुकूलन रणनीतियाँ

PCR has emerged as a common technique in many molecular biology laboratories. Provided here is a quick guide to several conventional PCR protocols. Because each reaction is a unique experiment, optimal conditions required to generate a product vary. Understanding the variables in a reaction will greatly enhance troubleshooting efficiency, thereby increasing the chance to obtain the desired result.