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DOI :
10.3791/4003-v
•
11:56 min
November 29th, 2012
Chapters
0:05
Title
2:12
Preparation of Lentivirus
3:24
Derivation of iPSC Lines
4:37
Generation of Tetraploid Blastocysts
7:20
Preparation of iPSCs for Blastocyst Injection
9:20
Results: Reprogramming MEFs to iPSCs
10:55
Conclusion
生成诱导多能干细胞(IPSC)线生产线,不同的发展潜力,即使当他们通过标准测试的多能性。在这里,我们描述了一个协议,全部来自iPS细胞拥有完整的多能性,它定义了IPSC的小鼠 1。
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