Name: retrograde loading of nerves, tracts, and spinal roots with fluorescent dyes
Uploaded: 2012-04-19T13:00:00
Description: Watch this Scientific Journal Video about Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes at JoVE.com

<p class="jove_content"&gt;この作品は、国立衛生研究所の神経疾患や脳卒中の国立研究所の学内プログラムによってサポートされていました。また、メソッドには、図3のデータへの彼の以前の貢献のために博士ジョージMentisに感謝します。</p

蛍光デキストランは、解剖学的ツールとして、イメージング神経活動1-4使用されています。フィールドら 、（2009）4は、モデル系として脊髄を中心とした軸索路にイオンと電位感受性色素を適用するためのプロトコルを公開しました。ここでは、 インビトロ optophysiologicalおよび形態学的研究のためにカット腹根、後根または脊髄のいずれかの神経管に蛍光及び/?...

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<p class="jove_content"&gt;ここでは神経細胞、神経や脊髄路に色素を導入するための簡単​​かつ費用対効果の高いプロトコルを記述します。このメソッドは、ロードプロセスの間、高濃度の色素溶液に識別解剖学的経路を公開することが含まれます。 microinjections、バス·アプリケーションおよびエレクトロポレーション法に比べて少し背景を持つターゲット·サイトの逆行性標識法で、この結果。しかし、この方?...

<table border="1"&gt;<tbody&gt;<tr&gt;<td colspan="2"<strong&gt;材料名</strong</td&gt;<td<strong&gt;会社概要</strong</td&gt;<td<strong&gt;カタログ番号</strong</td&gt;<td<strong&gt;コメント</strong</td&gt;</tr&gt;<tr&gt;<td rowspan="2"&gt;チューブ</td&gt;<td&gt; PE90（IDxOD：0.034 &quot;x0.050&quot;;壁Thikness：0.008 &quot;）</td&gt;<td&gt;クレイ·アダムスブランドIntermedic</td&gt;<td&gt; 427421</td&gt;<td&gt;タイプIとタイプIIピペット用</td&gt;</tr&gt;<tr&gt;<td&gt; NSF-51（IDxOD：1/16x1/8、壁Thikness：1/32）</td&gt;<td&gt; PharMed BPT、コール·パーマー</td&gt;<td&gt; AY242002</td&gt;<td&gt;フレキシブルチューブ用</td&gt;</tr&gt;<tr&gt;<td&gt;シリンジ</td&gt;<td&gt; 1ミリリットルのインスリンシリンジU-100</td&gt;<td&gt;ベクトン·ディッキンソン</td&gt;<td&gt; 329650</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;ニードル</td&gt;<td&gt; 19G X1-1/2 &quot;メタルハブニードル</td&gt;<td&gt; MONOJECT</td&gt;<td&gt; 200136</td&gt;<td&gt;シリンジにタイプIIのピペットを接続する</td&gt;</tr&gt;<tr&gt;<td&gt;</td&gt;<td&gt;アルコールランプ</td&gt;<td&gt;</td&gt;<td&gt;</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;コック</td&gt;<td男性ルアースリップアダプタを搭載した&gt;三方活栓</td&gt;<td&gt;バクスターヘルスケア（株）</td&gt;<td&gt; 2C6241</td&gt;<td&gt;注射器を使用します。</td&gt;</tr&gt;<tr&gt;<td rowspan="3"&gt;ホルダー</td&gt;<td&gt; H-1電極ホルダー</td&gt;<td&gt;ナリシゲ</td&gt;<td&gt; H-1/12</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;マグネットスタンド</td&gt;<td&gt;ナリシゲ</td&gt;<td&gt; GJ-8</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;マニピュレーター</td&gt;<td&gt;ナリシゲ</td&gt;<td&gt; M-3333</td&gt;<td&gt;</td&gt;</tr&gt;<tr&gt;<td&gt;ピンセット</td&gt;<td&gt; S＆Tデュモンスイス00632から11</td&gt;<td&gt;デュモン</td&gt;<td&gt; JF-5 TC</td&gt;<td&gt;</td&gt;</tr&gt;</tbody&gt;</table&gt;<p class="jove_content"&gt; *これらの提案した材料である。互換性のある任意の製品に置き換えることができます</p

retrograde loading of nerves, tracts, and spinal roots with fluorescent dyes

ニューロンの逆行性標識はまた、ニューロンの3月6日に、カルシウムや電位感受性色素をロードするために使用されている標準的な解剖学的方法1,2です。一般的に、色素が固体結晶として、あるいはガラスピペットを使用してローカルの圧力注入により適用されます。しかし、これはいくつかの時間が染料拡散するために必要な場合は特に、染料と還元の標識強度の希釈になる可能性があります。ここでは、色素溶液を充填したポリエチレン吸引ピペットを用いて神経細胞に蛍光灯やイオン感受性色素を導入するためのシンプルで低コストの手法を示しています。このメソッドは、ロード処理全体の軸索と接触している染料の高濃度を維持するための信頼できる方法を提供しています。

Watch this Scientific Journal Video about Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes at JoVE.com

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

我々は、ニューロンまたはポリエチレン吸引ピペットを使用して、任意の神経管に蛍光カルシウム感受性色素を高濃度に導入するための簡単​​かつ低コストの手法を説明します。

神経、地帯、および蛍光色素で脊髄根の逆行ローディング

Retrograde labeling of neurons is a standard anatomical method1,2 that has also been used to load calcium and voltage-sensitive dyes into neurons3-6. ...

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