Name: basic caenorhabditis elegans methods: synchronization and observation
Uploaded: 2012-06-10T14:00:00
Description: Watch this Scientific Journal Video about Basic Caenorhabditis elegans Methods: Synchronization and Observation at JoVE.com

作者想承认，AGAUR（博士奖学金到劳拉Fontrodona），MICINN（PTA项目配套蒙特塞拉特肝门德拉·里瓦），研究所的Salud的卡洛斯三世（米格尔Servet计划支持朱利安Cerón），玛丽·居里表意文字，ISCIII和IDIBELL融资实验室。

1。议定书：11漂白培养蠕虫 C.人口众多线虫可以得到培养他们无论是在液体介质或固体介质中板。它们通常生长在固体NGM的（线虫的生长介质）和美联储与E.大肠杆菌的细菌，它被添加到的板块无论是活着还是死了（由紫外线12死亡，13热或冷14）。最常见的程序使用现场OP50 E。大肠杆菌 ，这是在尿嘧啶合成缺陷，并?...

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线虫同步几个漂白解决方案已被描述。我们尝试不同的食谱（ 见表一 ），在我们的手中，他们没有表现出显着性差异在蠕虫人口同步（ 图1）。然而，我们的实验也表明，参数，如温度（ 图2），比漂白液：虫量（ 图3）和M9的体积与胚胎培养孵化（ 图4）做影响蠕虫的生存与文化的适当通风。在我们的晃动的条件?...

basic caenorhabditis elegans methods: synchronization and observation

到线虫的分子和发育生物学的研究，在七十年代初开始，由悉尼·布雷，它已被广泛用来作为模式生物1。C。线虫具有关键属性，如简单，透明和生命周期短，都使得它一个合适的2多年的基本生物学研究的实验系统。这种线虫的发现具有广泛的影响，因为许多细胞和分子过程控制动物发展进化保守。 通过胚胎阶段和四个幼虫进入前动物达到成年线虫的生命周期。发展可以采取2至4天，根据温度。在每一个阶段，可以观察到几个特征性状。其完整的细胞谱系知识4,5与深annotat的共同其基因组的离子变成一个领域模型这种线虫老化7,8神经生物学，干细胞生物学9和生殖细胞生物学10多元。 一个额外的功能，使三线虫一个有吸引力的工作模式是获得人口在一个特定的阶段同步蠕虫通过一个相对简单的协议的可能性。易于维护和传播这种线虫添加到同步的可能性提供一个功能强大的工具来获得大量的蠕虫病毒，它可以RNAi屏幕，芯片，大规模测序，如小或高通量实验的各种使用，免疫或原位杂交，等等。 由于其透明度，C。线虫结构可以区分使用微分干涉对比显微镜显微镜下，也Nomarski显微镜复制。利用DNA荧光的DAPI（4&#39;,6-diamidino-2-苯基）粘结剂，例如，可能会导致个别细胞的识别和定位，以及与他们相关的亚细胞结构/缺陷。

Watch this Scientific Journal Video about Basic Caenorhabditis elegans Methods: Synchronization and Observation at JoVE.com

Basic Caenorhabditis elegans Methods: Synchronization and Observation

维护和线虫传播的难易<em&gt; C。线虫</em&gt;使一个很好的模式生物工作。同步蠕虫病毒的可能性，使相当数量的科目，在同一发育阶段的工作，促进一个特定的进程，在许多动物的研究。

基本的<em&gt;线虫</em&gt;方法：同步和观察

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

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Biology

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

测量线虫固体介质的寿命

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

科研

生物学

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

分离和<em在体外激活线虫精子

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

实心板为基础的饮食限制在线虫</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

前列腺素的提取与分析<em&gt;线虫</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

观察与端粒的定量和重复序列用荧光<em&gt;原位</em&gt;杂交（FISH）与PNA探针<em&gt;秀丽隐杆线虫</em

Telomere is a ribonucleoprotein structure that protects chromosomal ends from aberrant fusion and degradation. Telomere length is maintained by telomerase ...

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans

方法研究线虫线虫中固有的蛋白质聚集与年龄的变化

In the last decades, the prevalence of neurodegenerative disorders, such as Alzheimer's disease (AD) and Parkinson's disease (PD), has grown. These ...

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

线虫的核苷酸和蛋白质提取联合

A single biological sample holds a plethora of information, and it is now common practice to simultaneously investigate several macromolecules to capture ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

线虫的耗氧率测定

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

卡诺尔哈布炎细胞外囊泡的纯化与分析

The secretion of small membrane-bound vesicles into the external environment is a fundamental physiological process of all cells. These extracellular ...

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

建模年龄相关的神经退行性疾病在 卡诺哈布迪蒂斯埃利根

Battling human neurodegenerative pathologies and managing their pervasive socioeconomic impact is becoming a global priority. Notwithstanding their ...