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DOI :
10.3791/4022-v
•
9:36 min
August 29th, 2012
Chapters
0:05
Title
1:06
Isolation of Brain Microvessels
4:35
Immortalizing the Brain Microvascular Endothelial Cells
7:22
Cultivating the Brain Microvascular Endothelial Cells
8:13
Results: Representative Images of cEND Cells
9:14
Conclusion
此方法描述了如何从小鼠脑微血管内皮细胞隔离和不朽。我们描述了一个一步一步的协议,从同质化的脑组织,消化步骤,播种和永生化的细胞。通常情况下,大约需要五周获得一个同质的,永生的微血管内皮细胞系。
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