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DOI :
10.3791/4022-v
•
9:36 min
August 29th, 2012
Chapters
0:05
Title
1:06
Isolation of Brain Microvessels
4:35
Immortalizing the Brain Microvascular Endothelial Cells
7:22
Cultivating the Brain Microvascular Endothelial Cells
8:13
Results: Representative Images of cEND Cells
9:14
Conclusion
この方法では、マウスの脳から微小血管内皮細胞を分離し不死化する方法について説明します。我々は、脳組織、消化手順、播種し、細胞の不死化の均質化から始まるステップ·バイ·ステップのプロトコルを記述します。通常、それは均質、不死化血管内皮細胞株を得るために約5週間かかります。
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