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DOI :
10.3791/4024-v
•
8:51 min
May 8th, 2012
Chapters
0:05
Title
1:34
Coating Surfaces with Matrix Ligand
2:54
Preparation of Cells
3:50
Preparing the Low-intensity Pulsed Ultrasound (LIPUS) Stimulation
5:10
Focal Adhesion Analysis by Immunofluorescence
5:58
Biochemical Assays
6:24
Representative Results
8:18
Conclusion
本协议描述成纤维细胞与低强度脉冲超声波的刺激,从而推动粘着形成和Rac1激活通过模仿跨膜基质受体,降解syndecan-4参与。这种方法允许在细胞水平上一个成功的临床技术的调查,从而提供了完善的治疗机会。
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