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DOI :
10.3791/4024-v
•
8:51 min
May 8th, 2012
Chapters
0:05
Title
1:34
Coating Surfaces with Matrix Ligand
2:54
Preparation of Cells
3:50
Preparing the Low-intensity Pulsed Ultrasound (LIPUS) Stimulation
5:10
Focal Adhesion Analysis by Immunofluorescence
5:58
Biochemical Assays
6:24
Representative Results
8:18
Conclusion
このプロトコルは、接着斑の形成と膜貫通マトリックス受容体、シンデカン-4の関与を模倣することによってRac1の活性化を駆動する低強度パルス超音波による培養線維芽細胞の刺激を説明しています。このアプローチは、それによって治療法の改良の機会を提供する、細胞レベルで成功した臨床技術の調査を可能にします。
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