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DOI :
10.3791/4029-v
July 16th, 2012
Chapters
0:05
Title
2:02
Induction of Fusion by PEG
5:04
Immunofluorescence to Determine Fusion Efficiency
7:21
Co-culture and Transfection of a Fusogenic PFV Glycoprotein
8:24
Infection Assay
9:33
Visualization of Cell Fusion
10:56
Results: Using Heterokaryon Formation to Assess the Influence of Dominant Restrictions on the HCV Life Cycle
16:23
Conclusion
我々は、条件は、次の2つの方法について説明します。トランス-相補性C型肝炎ウイルス(HCV)は、アセンブリとヘテロカリオンの形成に依存している完全なウイルスのライフサイクルの完了。これらの技術は、感染性HCV子孫の生産を妨げる主要な制限因子を発現する細胞系の画面に適しています。
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