Name: intraductal injection of lps as a mouse model of mastitis: signaling visualized via an nf-&kappa;b reporter transgenic
Uploaded: 2012-09-04T12:00:00
Description: Watch this Scientific Journal Video about Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-ÃŽÂºB Reporter Transgenic at JoVE.com

这项工作是由美国国立卫生研究院资助CA113734到F Yull和退伍军人事务部（TS布莱克韦尔）获得。

所有的动物实验，范德比尔特大学实验动物护理和使用委员会的批准。 1。准备转基因小鼠<ol><li> NGL（N F-κB-G FP-L uciferase）记者转基因（前面描述1）阳性的雌性小鼠，用于此过程。当女性的年龄至少6周，交配与FVB野生型男性。后宫养殖可以使用（在一个笼子里，男1例，女4例）。 （请注意：FVB应变小鼠用于目前的研究中，也可以使用较深的菌株如C57BL6?...

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在这里，我们利用转基因NF-κB记者的鼠标和优化注射技术模型乳腺炎，乳房组织的炎症最常见的原因是细菌感染。在此过程中最关键的一步是导管内注射本身。 在实践中，这通常也是显而易见注射导管内是否已经成功。注射剂（LPS或PBS）应该感到流体，应该有很少或几乎没有阻力的注射器被清空。最常见的陷阱是导致阻塞通道进入腺体，以乳头的损伤。这可以是可视化的寻?...

<table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> 大肠杆菌脂多糖 （LPS） </td><td>西格玛</td><td> ł2880 </td><td>血清型055：B5 </td></tr><tr><td> PBS </td><td>敏达公司</td><td> 46-013-CM </td><td></td></tr><tr><td> 31计胰岛素注射器</td><td> Becton Dickinson公司</td><td> 328438 </td><td>短针</td></tr><tr><td>镊子</td><td>世界精密仪器</td><td> 15908 </td><td></td></tr><tr><td>解剖范围</td><td>徕卡</td><td> M3Z </td><td></td></tr><tr><td>特雷尔异氟醚，USP </td><td>接收精英MINRAD INC。 </td><td> NDC 66794-011-25 </td><td></td></tr><tr><td> IVIS 200成像系统&lt;/ TD&gt; <td>显卡尺（原精诺真） </td><td> N / A </td><td></td></tr><tr><td>萤光素注射的注射器</td><td> Becton Dickinson公司</td><td> 309597 </td><td> 1CC 26G5 / 8; </td></tr><tr><td>萤火虫，D-荧光素钠盐一水合物 （荧光素基底） </td><td> Biosynth化学和生物</td><td> L-8240 </td><td>稀释至10毫克/毫升</td></tr></tbody></table>

intraductal injection of lps as a mouse model of mastitis: signaling visualized via an nf-&kappa;b reporter transgenic

疾病进展和相关的机制，严格学习阶段，最终的临床前模型测试干预是必要的，人类疾病的动物模型。在这些方法中，我们描述了一种技术，其中脂多糖（LPS）被注入到通过乳头，有效建模乳腺炎，或炎症，腺体泌乳小鼠乳腺。增加核因子kappa B（NF-κB）信号，这个模拟感染的结果，显示通过生物 ​​发光成像的NF-κB荧光素酶报告基因小鼠1。 我们的最终目标是研究开发这些方法有关的炎症，乳腺炎在哺乳期腺，通常包括发红，肿胀，免疫细胞浸润2,3。因此，我们敏锐地意识到，切口或任何类型的受伤的皮肤，乳头，或腺体介绍LPS可以在我们的方法中不能使用，因为该方法可能会混淆的读出的炎症。我们也想要一个直接的方法，并不需要特制的手绘移液器或利用显微操作器来保存这些专门的工具。因此，我们确定了一个完整的乳头进入乳腺导管，使用市售的胰岛素注射器和注射剂。此方法是成功的，并允许我们研究与LPS注射相关的炎症，由注射的过程中没有任何额外的效果重叠。此外，该方法还利用NF-κB的荧光素酶报告基因的转基因小鼠和生物发光成像技术的视觉和定量显示增加的NF-κB信号在LPS注射压盖4。 谁愿意在哺乳期乳腺疾病模型，因为归根结底，这项技术的许多学科的研究人员感兴趣的是这些方法这里描述的可用于注射的物质的数量，并不限定于仅LPS。

Watch this Scientific Journal Video about Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-ÃŽÂºB Reporter Transgenic at JoVE.com

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-ÃƒÅ½Ã‚ÂºB Reporter Transgenic

这里描述的是一种技术，其中脂多糖注入泌乳小鼠乳腺通过乳头模拟乳腺炎，通常由细菌感染引起的一个条件。脂多糖（LPS）的结果中增加核因子kappa B（NF-κB）信号，通过NF-κB荧光素酶报告基因小鼠的生物发光成像的可视化。

导管内可视化通过NF-κB记者的转基因小鼠模型的乳腺炎：信号注射LPS

Animal models of human disease are necessary in order to rigorously study stages of disease progression and associated mechanisms, and ultimately, as ...

Research

JoVE Journal

Medicine

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-&kappa;B Reporter Transgenic

Mouse Bladder Wall Injection

鼠标膀胱壁注射液

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. ...

科研

医学

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

采用实时定量PCR检测供体细胞植入一个具有竞争力的小鼠骨髓移植模型

Murine bone marrow transplantation models provide an important tool in measuring hematopoietic stem cell (HSC) functions and determining genes/molecules ...

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke

脑Microslices的兴奋性刺激作为<em&gt;在体外</em&gt;中风模型

Examining molecular mechanisms involved in neuropathological conditions, such as ischemic stroke, can be difficult when using whole animal systems. As ...

A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys

A Possible Zebrafish Model of Polycystic Kidney Disease: Knockdown of wnt5a Causes Cysts in Zebrafish Kidneys

多囊肾一种可能的斑马鱼型号：击倒<em&gt; Wnt5a的</em&gt;原因在斑马鱼肾囊肿

Polycystic kidney disease (PKD) is one of the most common causes of end-stage kidney disease, a devastating disease for which there is no cure. The ...

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

通过重组腺相关病毒载体注射立体的α-突触核蛋白基于大鼠模型为帕金森氏病的发展

In order to study the molecular pathways of Parkinson&#39;s disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on ...

Induction and Micro-CT Imaging of Cerebral Cavernous Malformations in Mouse Model

小鼠脑海绵体畸形的诱导及显微 CT 成像

Mutations in the CCM1 (aka KRIT1), CCM2, or CCM3 (aka PDCD10) gene cause cerebral cavernous malformation (CCM) in humans. Mouse models of CCM disease have ...

Puncture-Induced Iris Neovascularization as a Mouse Model of Rubeosis Iridis

穿刺诱导的虹膜新生血管虹膜虹膜小鼠模型的建立

We describe a model of puncture-induced iris neovascularization as a general model for noninvasive evaluation of angiogenesis. The model is also relevant ...

Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome

油酸注射液在猪急性呼吸窘迫综合征模型中的研究

The acute respiratory distress syndrome is a relevant intensive care disease with an incidence ranging between 2.2% and 19% of intensive care unit ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

用线 Myography 测定小鼠肠系膜动脉的等轴收缩力

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

毛利精密切肝片作为肝脏生物学的Ex Vivo模型

Understanding the mechanisms of liver injury, hepatic fibrosis, and cirrhosis that underlie chronic liver diseases (i.e., viral hepatitis, non-alcoholic ...