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DOI :
10.3791/4031-v
May 24th, 2012
Chapters
0:05
Title
1:24
Packaging of Lentiviral Vectors
3:28
Concentration and Purification of the Vectors
4:50
Titration of the Vectors
6:25
Transduction of Neocortical Cultures
7:20
Transduction Results
8:25
Conclusion
在这个协议中,我们描述了慢病毒载体的生产,提纯和滴定。我们提供了一个在原代培养的神经元和神经胶质细胞的慢病毒载体介导的基因传递的例子。我们的方法也适用于其他类型的细胞在体外和在体内。
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