Name: collection protocol for human pancreas
Uploaded: 2012-05-23T12:00:00
Description: Watch this Scientific Journal Video about Collection Protocol for Human Pancreas at JoVE.com

作者感谢捐助者的家庭和组织的专家协助参与这项研究和玛丽亚·马蒂诺和伊琳娜Kusmartseva器官采购。这项工作是由青少年糖尿病研究基金会（MC-T.，马萨诸塞州）在对糖尿病患者的胰腺器官捐赠者的网络支持。

1。设置<ol><li>石蜡块，用铅笔或自动录像带打印机手动标签的录像带。包括捐赠标识符（CaseID），样本类型，等分数目，以及任何额外的唯一标识（案例表， <a href="http://jove.com/files/ftp_upload/4039/4039appendix1.doc" target="_blank">附录1</a> ）。 </li><li>标签华侨城模具为永久性标记录音带。剪下铝箔5-10厘米的长方形。 </li><li>打印或手工标签永久性标记所有小瓶和管。包括足够瓶碎单元冷冻样品和新鲜出?...

<ol>
	<li>Brissova, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+human+pancreatic+islet+architecture+and+composition+by+laser+scanning+confocal+microscopy.">Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy.</a> J. Histochem. Cytochem. 53, 1087-1097 (2005).</li><li>Rahier, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pancreatic+polypeptide+cells+in+the+human+pancreas:+the+effects+of+age+and+diabetes.">The pancreatic polypeptide cells in the human pancreas: the effects of age and diabetes.</a> J. Clin. Endocrinol. Metab. 56, 441-444 (1983).</li><li>Uchida, T., Takada, T., Ammori, B. J., Suda, K., Takahashi, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+reconstruction+of+the+ventral+and+dorsal+pancreas:+a+new+insight+into+anatomy+and+embryonic+development.">Three-dimensional reconstruction of the ventral and dorsal pancreas: a new insight into anatomy and embryonic development.</a> J. Hepatobiliary Pancreat. Surg. 6, 176-180 (1999).</li><li>Wittingen, J., Frey, C. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Islet+concentration+in+the+head,+body,+tail+and+uncinate+process+of+the+pancreas.">Islet concentration in the head, body, tail and uncinate process of the pancreas.</a> Ann. Surg. 179, 412-414 (1974).</li><li>Rahier, J., Goebbels, R., Henquin, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+composition+of+the+human+diabetic+pancreas.">Cellular composition of the human diabetic pancreas.</a> Diabetologia. 24, 366-371 (1983).</li><li>Vaught, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+NCI+perspective+on+creating+sustainable+biospecimen+resources.">An NCI perspective on creating sustainable biospecimen resources.</a> J. Natl. Cancer Inst. Monogr. 42, 1-7 (2011).</li><li>Gianani, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Initial+results+of+screening+of+nondiabetic+organ+donors+for+expression+of+islet+autoantibodies.">Initial results of screening of nondiabetic organ donors for expression of islet autoantibodies.</a> J. Clin. Endocrinol. Metab. 91, 1855-1861 (2006).</li><li>Tauriainen, S., Salmela, K., Rantala, I., Knip, M., Hy&#246;ty, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Collecting+high-quality+pancreatic+tissue+for+experimental+study+from+organ+donors+with+signs+of+&#946;-cell+autoimmunity.">Collecting high-quality pancreatic tissue for experimental study from organ donors with signs of &#946;-cell autoimmunity.</a> Diabetes Metab. Res. Rev. 26, 585-592 (2010).</li><li>In't Veld, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Screening+for+insulitis+in+adult+autoantibody-positive+organ+donors.">Screening for insulitis in adult autoantibody-positive organ donors.</a> Diabetes. 56, 2400-2404 (2007).</li><li>T&#246;ns, H. A., Terpstra, O. T., Bouwman, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heterogeneity+of+human+pancreata+in+perspective+of+the+isolation+of+the+islets+of+langerhans.">Heterogeneity of human pancreata in perspective of the isolation of the islets of langerhans.</a> Transplant Proc. 40, 367-369 (2008).</li></ol>

此过程的目标是定义一个标准的胰腺处理，可以跨多个收集点统一，从而使不同群体得到的结果进行比较。剥离的方法是基于标准的注释主要胰腺区域内细分区域的额外步骤与手术病理做法。标准化的biospecimen处理方法是必需的，以便收集高品质的最佳做法biospecimen收集出版由国家组织，如国家癌症研究所6 biobanking体现样品。从捐助者代表糖尿病和适当的控制人口的各个阶段的人类胰腺样?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>清扫工具，包括22个刀片的手术刀</td><td>各个</td><td>各个</td><td></td></tr><tr><td>离心管（15和50毫升） </td><td> Fisher Scientific则</td><td> 05-539-5，430828 </td><td>不育</td></tr><tr><td> cryotubes（1.8毫升） </td><td>厂商VWR北美国</td><td> 89004-300 </td><td>无菌，条形码可选</td></tr><tr><td> RNAlater </td><td> Ambion公司</td><td> AM7021 </td><td></td></tr><tr><td>外科病理学油墨</td><td></td><td></td><td></td></tr><tr><td>录音带</td><td>奔驰医学</td><td>中科院ECO109 </td><td></td></tr><tr><td> 10％的中性福尔马林</td><td> Fisher Scientific则</td><td> 23-245-685 </td><td></td></tr><tr><td>华侨城冻结媒体</td><td> TissueTek </td><td> 4583 </td><td></td></tr><tr><td>模具</td><td> ThermoScientific </td><td> 58952 </td><td>各种规格</td></tr><tr><td>异戊烷</td><td> Fisher Scientific则</td><td> O3551-4 </td><td>华侨城低温浴</td></tr><tr><td>干冰</td><td></td><td></td><td>华侨城低温浴</td></tr><tr><td>液氮杜瓦瓶</td><td></td><td></td><td>抢购冷冻冷冻管</td></tr><tr><td>细胞培养皿（100毫米） </td><td>康宁</td><td> 430167 </td><td>不育</td></tr><tr><td>胎牛血清DMEF </td><td> ThermoScientific </td><td> SH30023.01 </td><td>谷氨酰胺包括无菌，储存冷藏， </td></tr><tr> &lt;TD&gt;胎牛血清</td><td> cellgro </td><td> 35-010-CV </td><td>无菌，冷冻分装店</td></tr><tr><td>青霉素/ /真菌</td><td> GIBCO公司</td><td> 15240-062 </td><td>无菌，冷冻分装店</td></tr><tr><td>键入9石蜡</td><td>理查德 - 阿兰科学</td><td> 8337 </td><td></td></tr><tr><td> 1XÐ-PBS </td><td> GIBCO公司</td><td> 14190-144 </td><td>不育</td></tr><tr><td> 16％多聚甲醛</td><td>新兴科学</td><td> 15710 </td><td>新鲜，商店冷藏</td></tr><tr><td> 8％的戊二醛</td><td>新兴科学</td><td> 16019 </td><td>新鲜，商店冷藏</td></tr><tr><td>生物安全等级2罩</td><td>各个</td><td></td><td></td></tr><tr><td>超低冰柜</td><td>各个</td><td></td><td></td></tr><tr><td>樱花纸盒打印机</td><td>樱花</td><td></td><td></td></tr><tr><td>樱花VIP300 </td><td>樱花</td><td></td><td>自动石蜡处理器的</td></tr><tr><td>石蜡包埋站</td><td> ThermoFisher </td><td></td><td></td></tr></tbody></table> 表1。特定的试剂和设备。

collection protocol for human pancreas

这解剖和取样程序开发为糖尿病（nPOD）的计划，以规范准备恢复从尸体器官捐献者的胰腺胰腺器官捐赠者的网络。胰腺分为3个主要地区整个横向轴内侧串行的横截面（头，身，尾）。交替部分用于固定石蜡和鲜冻块和残留样品剁碎单元冷冻样品准备，无论有或无RNase抑制剂，DNA，RNA或蛋白质隔离。胰腺的解剖过程的总体目标是保持解剖定位的同时，品尝整个胰腺。 在小岛组成，大小和数字方面的内分泌细胞的异质性是人类胰岛的报告相比，啮齿动物胰岛1。大多数人类胰岛胰头，身体和尾巴地区组成的胰岛素containinGβ-细胞的比例较低，其次是胰高血糖素的α细胞和生长抑素的δ-细胞。胰多肽含PP细胞生长素含小量细胞也存在，但在小数字。相比之下，钩突地区包含主要胰岛胰多肽含PP细胞组成。这些区域的胰岛变化，从发展的差异产生。胰腺发展的前肠和胃和十二指肠，腹侧叶动作和保险丝背3旋转后的腹侧和背侧胰芽。腹侧叶形成的头后部，包括钩突背叶而产生的其他器官。另据报道，尾部区域，具有较高的胰岛细胞密度比其他地区和背叶源性成分，在1型糖尿病的发生选择性萎缩区域胰腺癌的变化<s高达&gt; 4,5。 其他器官和组织往往是恢复从器官捐赠者和包括胰腺淋巴结，脾脏和非胰腺淋巴结。这些样品被收回冻存细胞的分离此外胰腺类似的格式。当近端十二指肠与胰腺，十二指肠粘膜可收集石蜡和冰冻块和碎单元冷冻的筹备工作。

Watch this Scientific Journal Video about Collection Protocol for Human Pancreas at JoVE.com

Collection Protocol for Human Pancreas

该视频演示了加工成多种存储格式的人类胰腺的解剖过程。解剖方向，保持整个胰腺地区允许区域胰岛成分和密度的定义。

人体胰腺的收集协议

This dissection and sampling procedure was developed for the Network for Pancreatic Organ Donors with Diabetes (nPOD) program to standardize preparation ...

Research

JoVE Journal

Medicine

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从手术标本的人类胰岛细胞激光显微切割的改进方法

Laser microdissection (LMD) is a technique that allows the recovery of selected cells and tissues from minute amounts of parenchyma 1,2. The dissected ...

科研

医学

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

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Perspiration monitoring can be utilized for the detection of certain diseases, such as thermoregulation and mental disorders, particularly when the ...

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

应用改良袖套技术的小鼠胰腺移植模型

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the ...

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We have been investigating the pancreas specific transcription factor, 1a cre-recombinase; lox-stop-lox- Kristen rat sarcoma, glycine to aspartic acid at ...

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慢性胰腺炎的机器人侧胰腺肌切除术

Lateral pancreaticojejunostomy (LPJ) has shown good postoperative outcomes in patients with painful, morphine dependent, chronic pancreatitis (CP). The ...

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胰腺癌的腹腔镜根左造影切除术：手术策略与技术视频

Radical resection margins, resection of Gerota&#8217;s (perirenal) fascia, and adequate lymph node dissection are crucial for an adequate oncological ...

Robot-Assisted Kidney Transplantation

机器人辅助肾移植

This paper describes robot-assisted kidney transplantation (RAKT) from a living donor. The robot is docked between the parted legs of the patient, placed ...

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

Stabilized Longitudinal In Vivo Cellular-Level Visualization of the Pancreas in a Murine Model with a Pancreatic Intravital Imaging Window

稳定纵向 在Vivo 细胞级可视化胰腺在穆林模型与胰腺内活性成像窗口

Direct in vivo cellular-resolution imaging of the pancreas in a live small animal model has been technically challenging. A recent intravital imaging ...

Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency

Measurements of cilia function (beat frequency, pattern) have been established as diagnostic tools for respiratory diseases such as primary ciliary ...