Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
20.8K Views
•
11:23 min
May 22nd, 2012
DOI :
10.3791/4045-v
Chapters
0:05
Title
1:39
Cell Preparation
3:18
Harvesting and Fixation
5:09
BrdU and PI Staining
7:01
Flow Cytometry
9:55
Analysis of Cell Cycle Progression: Representative Results
11:07
Conclusion
Summary
在细胞周期各阶段的进展和动力学跟踪微妙的变化,可以实现通过碘化丙啶与BrdU和总DNA染色核酸代谢标记的组合使用。这种方法避免了化学循环细胞同步的需要,从而防止非特异性的DNA损伤,这反过来又影响细胞周期进程的介绍。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved