Name: functional imaging of brown fat in mice with 18f-fdg micro-pet/ct
Uploaded: 2012-11-23T12:00:00
Description: Watch this Scientific Journal Video about Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT at JoVE.com

作者要感谢劳拉·迪亚斯，凯文·菲利普斯，薇拉薛，王C.李的宝贵意见和技术支持，在发展这种方法。

1。动物的制备和冷处理<ol><li>找到并检查了4℃冷室中，已被批准用于容纳实验室小鼠。 </li><li>预冷动物的笼子，在寒冷的房间里过夜。网箱组装饲料和床上用品，但没有一瓶水。 </li><li>在的实验一天早上，地方小鼠一个由一个以30分钟的间隔到各预冷的笼子。每个单笼的鼠标应该留在寒冷的房间了近4个小时，然后才运到的成像实验室。确保小鼠禁食，但获得水。 </li><li>后4小时冷处理运输动?...

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在这项研究中的一个micro-PET/CT-based成像方法已发展为在成年小鼠中检测BAT活动只需要一个冷处理和注射市售18 F-FDG。整个的过程可以在一天内完成后开始，每30分钟，直到所有的动物处理和成像处理和成像顺序。共10只小鼠（或2组，每组5只小鼠），在实验条件下，可以在同一天进行测试，用一个单一的成像系统。如果2个或更多的动物可以在一个特别设计的动物床同时扫描，因为它先前已?...

<table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td> Micro-PET/CT成像系统</td><td>西门子医疗解决方案（美国）公司</td><td> Inveon专用PET系统和的Inveon多模式CT / SPECT系统（停靠） </td><td></td></tr><tr><td>普萘洛尔</td><td>西格玛</td><td> P0884 </td><td></td></tr><tr><td> BRL 37344 </td><td>西格玛</td><td> B169 </td><td></td></tr><tr><td> 18 F-FDG </td><td> Cyclotope公司</td><td></td><td></td></tr><tr><td> C57BL/6J雄性小鼠</td><td>杰克逊实验室</td><td> 000664 </td><td> 3-4个月</td></tr></tbody></table>

functional imaging of brown fat in mice with 18f-fdg micro-pet/ct

其离散的位置，由于丰富的血管和高密度的线粒体和棕红色，褐色脂肪组织（BAT）与白色脂肪组织（WAT）。在新生哺乳动物的能量消耗和非颤抖性产热以及成人1 BAT发挥了重要作用。 BAT-介导的产热是由交感神经系统高度管制的，主要是通过β肾上腺素能受体2，3。最近的研究显示，英美烟草公司活动呈负相关，与成人的身体质量指数（BMI）和其他糖尿病参数4-6。因此，英美烟草公司提出专注于调制的能量平衡6-8 anti-obesity/anti-diabetes治疗的潜在目标。几个冷的挑战正电子发射断层扫描（PET）方法，建立了用于检测人BAT 9-13，基本上是没有什么标准协议的成像的量化ication的小动物模型，如小鼠BAT。在这里，我们描述了一个强大的PET / CT成像方法在小鼠BAT功能评估。简单地说，成年小鼠冷处理在空腹状态下，持续时间4小时之前，他们收到了一剂18 F-脱氧葡萄糖（FDG）。将小鼠保持在寒冷的一个额外小时后FDG注射，然后一个小的动物专用micro-PET/CT系统与扫描。所收购的PET图像共同登记的的CT图像解剖引用，并分析了FDG摄取在肩胛间BAT区提出BAT活动。这种标准化的冷处理和成像协议已经验证通过测试BAT活动，例如，在药物干预的抑制的BAT活化治疗β-受体阻滞剂心得安，14，15，或增强BAT激活β3受体激动剂BRL37344 16。法德cribed在这里可以应用到屏幕的药物/化合物，调节BAT的活动，或确定基因/ BAT各种临床前研究和基础研究的发展和法规中所涉及的途径。

小鼠BAT micro-PET/CT成像的一个例子示出在图1中。虽然CT成像提供解剖信息，PET成像编码的18 F-FDG摄取的分布和数量在整个身体。这些成像数据可以被分开（图1A和图1B），稠合的（1C），如最大强度投影（MIP，1D）与3D特征或演示。随着三维成像工具，感兴趣的体积（VOI）的帮助下，肩胛间BAT区域（ 图1中箭头所示），在这里上绘制的PET图像和总信号内的VOI％ID可以被?...

Watch this Scientific Journal Video about Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT at JoVE.com

Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT

功能成像的小鼠棕色脂肪组织（BAT）的方法进行了说明，冷刺激BAT摄取18F-脱氧葡萄糖（FDG）的非侵入性评估与标准化的micro-PET/CT协议。这种方法的鲁棒性和敏感的检测BAT活动在小鼠模型中的差异。

功能成像在小鼠体内的棕色脂肪与FDG micro-PET/CT

Brown adipose tissue (BAT) differs from white adipose  tissue (WAT) by its discrete location and a brown-red color due to rich  vascularization and high ...

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Functional Imaging of Brown Fat in Mice with 18F-FDG micro-PET/CT

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