Name: hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing
Uploaded: 2012-06-01T13:00:00
Description: Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

桑迪亚是multiprogram的实验室桑迪亚公司是洛克希德 - 马丁公司，美国能源部下属的国家核安全管理局根据合同DE-AC04-94AL85000经营。作者们承认，资金由美国桑迪亚国家实验室的实验室指导研究和开发（LDRD）计划。

1。微针的制备<ol><li>使用三维造型软件SolidWorks（Dassault Systemes的SA，韦利济，法国），设计一个金字塔形的空心微针阵列（ 图1）。3-5 </li><li>为支持使用魔法的RP 13软件（Materialise的内华达州，比利时鲁汶）微针阵列结构设计。支撑结构，使树脂从设备在制造过程中流失，并提供了一​​个建立基地的微针。一个例子支持结构如图1所示。 </li><li>链接的支持和微?...

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基于这种微针传感器的设计的多个方面被认为是前设备制造。为了使用这种传感器，实时检测传感器的响应时间必须是低的，在这个协议中，每个测试传感器具有响应时间低于十五秒。其选择性体内环境，其中包含可以干扰与电极反应的电化学生物分子内也选择在本协议中使用的浆料。除了粘贴组成，经营潜力的选择，以尽量减少干扰电活性的影响。微针阵列的制造成功，需要选择一个合?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>扁平电缆</td><td>莫仕</td><td> 3302/10SF </td></tr><tr><td> 0.003“侧双面胶带</td><td>聚酯薄膜</td><td></td></tr><tr><td> 0.004“双面胶带</td><td>聚酯薄膜</td><td></td></tr><tr><td>乳酸氧化酶</td><td>西格玛</td><td> L0638 </td></tr><tr><td>葡萄糖氧化酶</td><td>西格玛</td><td> G7141 </td></tr><tr><td>铑碳</td><td>西格玛</td><td> 206164 </td></tr><tr><td>石墨粉末</td><td>西格玛</td><td> 385031000 </td></tr><tr><td>聚（亚胺） </td><td>阿库罗斯福冈</td><td> 178570010 </td></tr><tr><td>矿物油</td><td>西格玛</td><td> M5904 </td></tr><tr><td>葡萄糖</td><td>西格玛&lt;/ TD&gt; <td> G8270 </td></tr><tr><td>乳酸</td><td>西格玛</td><td> L1750 </td></tr><tr><td>快速蓝RR盐</td><td>西格玛</td><td> F0500 </td></tr><tr><td> E-壳牌300 </td><td> EnvisionTEC </td><td></td></tr><tr><td> E-壳牌200 </td><td> EnvisionTEC </td><td></td></tr><tr><td>银/氯化银参比电极</td><td>巴斯</td><td> MF-2052 </td></tr><tr><td>铂丝</td><td>巴斯</td><td></td></tr><tr><td> PGSTAT12 AutolabPotentiostat </td><td> EcoChemie </td><td></td></tr><tr><td> perfactory反相</td><td> EnvisionTEC </td><td></td></tr><tr><td> ottoflash Postcuring系统</td><td> EnvisionTEC </td><td></td></tr><tr><td>磷酸</td><td>费舍尔</td><td> A366-4 </td></tr><tr><td> 60W型号6.75 CO 2的光栅/矢量激光系统</td><td> UNIVERSAL激光系统</td><td> PLS6.75 </td></tr><tr><td> CorelDraw中</td><td> Corel公司</td><td></td></tr><tr><td> Solidworks的</td><td> Dassault Systemes的</td><td> 2009 </td></tr><tr><td>魔法RP13 </td><td> Materialise的</td><td></td></tr></tbody></table>

hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing

一种微创复为有关生物分子进行快速分析监测系统的发展，可以提供个人患有慢性疾病的简便评估其直接的生理状态。此外，它可以作为一个复杂的，多因素的医疗条件的分析研究工具。为了实现这样一个多分析传感器，它必须是微创，组织间液的采样必须出现无疼痛或伤害用户，并分析必须迅速以及选择性。 最初发达，无痛苦的给药微针已被用于提供疫苗和药物制剂（如胰岛素）通过皮肤1-2由于这些设备访问的间隙，用微电极集成的微针可以作为透皮电化学传感器。选择性检测葡萄糖，谷氨酸，乳酸，Hydrogen过氧化氢 ​​和抗坏血酸已被证明使用碳纤维，修饰碳糊，铂金涂层的聚合物微针作为换能元件的集成微针电极的装置。3-7,8 这个微针的传感器技术，使一个新的和先进的分析方法，在原位，同时检测多种分析物。复用提供了复杂的微环境监测，否则很难在一个快速，微创的方式来描述的可能性。例如，这项技术可以被利用外同步监测，血糖，乳酸和pH值，9是重要的代谢疾病状态7,10-14指标（如癌症扩散）和运动诱发性酸中毒15。

Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

本文详细介绍了建设一个复用微针为基础的传感器。该设备正在研制多种分析快速和选择性地在现场采样和电化学分析。我们设想，临床医学和生物医学研究，这些微针为基础的传感器使用。

复透皮电化学传感的空心微针为基础的传感器

The development of a minimally invasive multiplexed monitoring system for rapid analysis of biologically-relevant molecules could offer individuals ...

Research

JoVE Journal

Bioengineering

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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

生物分子检测的干涉反射成像传感器（IRIS）

The sensitive measurement of biomolecular interactions has use in many fields and industries such as basic biology and microbiology, ...

科研

生物工程

Bacterial Detection &amp; Identification Using Electrochemical Sensors

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使用电化学传感器的细菌检测和鉴定

Electrochemical  sensors are widely used for rapid and accurate measurement of blood glucose and  can be adapted for detection of a wide variety of ...

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

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The gastrointestinal tract is an example of barrier tissue that provides a physical barrier against entry of pathogens and toxins, while allowing the ...

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

电动细胞基质阻抗传感的内皮细胞增殖，屏障功能的定量和能动性

Electric Cell-substrate Impedance Sensing (ECIS) is an in vitro impedance measuring system to quantify the behavior of cells within adherent cell layers. ...

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

微流体为基础的电化学生物芯片的无标记的DNA杂交分析

Miniaturization of analytical benchtop procedures into the micro-scale provides significant advantages in regards to reaction time, cost, and integration ...

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

生物膜制作的溶剂辅助脂质双分子层（SALB）方法

In order to mimic cell membranes, the supported lipid bilayer (SLB) is an attractive platform which enables in vitro investigation of membrane-related ...

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

中空纤维生物反应器的<em&gt;在体内</em&gt;般的哺乳动物组织培养

Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage ...

Extraction of Plant-based Capsules for Microencapsulation Applications

基于植物的提取胶囊微胶囊化应用

Microcapsules derived from plant-based spores or pollen provide a robust platform for a diverse range of microencapsulation applications. Sporopollenin ...

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

与复用电子检测的粒子的空间跟踪微流体平台

Microfluidic processing of biological samples typically involves differential manipulations of suspended particles under various force fields in order to ...

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

无交叉反应和无免疫多路复用夹芯芯片

Multiplexed protein analysis has shown superior diagnostic sensitivity and accuracy compared to single proteins. Antibody microarrays allow for thousands ...