Name: hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing
Uploaded: 2012-06-01T13:00:00
Description: Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

Sandia er multiprogram laboratorium, der drives af Sandia Corporation, en Lockheed Martin selskab, for Det Forenede Sagt på Department of Energy &#39;s nationale nukleare Security Administration i henhold til kontrakt DE-AC04-94AL85000. Forfatterne anerkender støtte fra Sandia National Laboratories &#39;Laboratory Directed Research &amp; Development (LDRD) program.

1. Mikro Fabrication <ol><li> Brug tre-dimensionel modellering software Solidworks (Dassault Systemes SA, Velizy, Frankrig), designe en pyramideformet-formet hul mikronålarray (figur 1). 3-5 </li><li> Design en støtte struktur for mikronålarray bruge magi RP 13 software (Materialise NV, Leuven, Belgien). Bærestrukturen tillader harpiksen at dræne fra indretningen under fremstilling og tilvejebringer en base, hvor mikronålene er bygget. Et eksempel bærestruktur er vist i figur...

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Flere aspekter af udformningen af ​​denne mikronålen-baserede sensor blev anset før enhed fabrikation. For at kunne bruge denne sensor for real-time detektering, skal responstiden for sensoren være lav, i denne protokol, udstillet hver testet sensor en responstid under femten sekunder. Indsætter benyttes i denne protokol blev også valgt for deres selektivitet i in vivo-miljøer, som indeholder elektroaktive biomolekyler, der kan forstyrre elektrode svar. Ud over pastasammensætning blev drift potential...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td></tr><tr><td> Flad fleksibelt kabel </td><td> Molex </td><td> 3302/10SF </td></tr><tr><td> 0,003 &quot;Side klæbebånd </td><td> Melinex </td><td></td></tr><tr><td> 0,004 &quot;dobbeltklæbende tape </td><td> Melinex </td><td></td></tr><tr><td> Lactatoxidase </td><td> Sigma </td><td> L0638 </td></tr><tr><td> Glucoseoxidase </td><td> Sigma </td><td> G7141 </td></tr><tr><td> Rhodium på carbon </td><td> Sigma </td><td> 206164 </td></tr><tr><td> Grafitpulver </td><td> Sigma </td><td> 385031000 </td></tr><tr><td> poly (ethylenimin) </td><td> Acros </td><td> 178570010 </td></tr><tr><td> Mineralolie </td><td> Sigma </td><td> M5904 </td></tr><tr><td> Glucose </td><td> Sigma &lt;/ Td&gt; <td> G8270 </td></tr><tr><td> Laktat </td><td> Sigma </td><td> L1750 </td></tr><tr><td> Fast Blue RR salt </td><td> Sigma </td><td> F0500 </td></tr><tr><td> e-Shell 300 </td><td> EnvisionTEC </td><td></td></tr><tr><td> e-Shell 200 </td><td> EnvisionTEC </td><td></td></tr><tr><td> Ag / AgCI referenceelektrode </td><td> Basi </td><td> MF-2052 </td></tr><tr><td> Pt-tråd </td><td> Basi </td><td></td></tr><tr><td> PGSTAT12 AutolabPotentiostat </td><td> EcoChemie </td><td></td></tr><tr><td> Perfactory RP </td><td> EnvisionTEC </td><td></td></tr><tr><td> Ottoflash Postcuring systemet </td><td> EnvisionTEC </td><td></td></tr><tr><td> Phosphorsyre </td><td> Fisher </td><td> A366-4 </td></tr><tr><td> 60W Model 6,75 CO 2 raster / vektor lasersystem </td><td> Universitetsal Laser Systems </td><td> PLS6.75 </td></tr><tr><td> CorelDraw </td><td> Corel </td><td></td></tr><tr><td> Solidworks </td><td> Dassault Systemes </td><td> 2009 </td></tr><tr><td> Magi RP13 </td><td> Materialise </td><td></td></tr></tbody></table>

hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing

Udviklingen af ​​en minimalt invasiv multiplex overvågningssystem for hurtig analyse af biologisk relevante molekyler kunne tilbyde personer, der lider af kroniske medicinske tilstande facile vurdering af deres umiddelbare fysiologiske tilstand. Desuden kunne det tjene som et forskningsværktøj til undersøgelse af komplekse, multifaktoriel medicinske tilstande. For en sådan multianalyte sensor skal udnyttes, skal det være minimalt invasiv, prøveudtagning af interstitielt fluid skal forekomme uden smerte eller skade på brugeren, og analyse skal være hurtig og selektiv. Oprindeligt udviklet til smertefri lægemiddeladministration, er mikronåle blevet anvendt til at levere vacciner og farmakologiske midler (fx insulin) gennem huden. 1-2 Da disse indretninger åbne interstitielle rum kan mikronåle, der er integreret med mikroelektroder anvendes som transdermal elektrokemiske sensorer. Selektiv detektering af glucose, glutamat, lactat, hydrogen peroxid og ascorbinsyre er blevet demonstreret ved hjælp af integrerede mikronål-elektrodeanordninger med carbonfibre, modificerede carbon pastaer og platin-coatede polymer mikronåle, der tjener som transducerende elementer. 3-7,8 Dette mikro-sensor-teknologi har muliggjort en ny og sofistikeret analytisk tilgang til in situ og samtidig påvisning af flere analytter. Multipleksing giver mulighed for at overvåge komplekse mikromiljøer, som ellers er vanskelige at karakterisere en hurtig og minimalt invasiv måde. For eksempel kunne denne teknik anvendes til samtidig monitorering af ekstracellulære niveauer af glucose, lactat og pH 9, som er vigtige metaboliske indikatorer af sygdomstilstande 7,10-14 (f.eks cancerproliferation) og anstrengelsesudløst acidose. 15

Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Denne artikel indeholder oplysninger om opførelse af en multiplex mikronål-baserede sensor. Indretningen bliver udviklet til in situ prøveudtagning og elektrokemisk analyse af flere analytter i en hurtig og selektiv måde. Vi forestiller klinisk medicin og biomedicinsk forskning anvender for disse mikro-baserede sensorer.

Hollow mikro-baserede sensor for Multiplexed Transdermal Elektrokemisk Sensing

The development of a minimally invasive multiplexed monitoring system for rapid analysis of biologically-relevant molecules could offer individuals ...

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