Name: hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing
Uploaded: 2012-06-01T13:00:00
Description: Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

Sandia er multiprogram laboratorium som drives av Sandia Corporation, en Lockheed Martin Company, for United Stated Department of Energys National Nuclear Security Administration under kontrakt DE-AC04-94AL85000. Forfatterne erkjenner midler fra Sandia National Laboratories &#39;Laboratory Regi Research &amp; Development (LDRD) program.

1. Microneedle Fabrication <ol><li> Ved hjelp av tredimensjonale modellering programvare Solidworks (Dassault Systemes SA, Velizy, Frankrike), designe en pyramideformet-formet hul microneedle array (figur 1). 3-5 </li><li> Lag en støtte struktur for microneedle matrisen bruke magi RP 13 programvare (Materialise NV, Leuven, Belgia). Støtten strukturen tillater harpiksen renne fra enheten under fabrikasjon og gir en base som de microneedles er bygget. Et eksempel støtte struktur er vist i ...

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Flere aspekter ved utformingen av dette microneedle-baserte sensor ble vurdert før enheten fabrikasjon. For å bruke denne sensoren for sporing i sanntid, må responstiden på sensoren være lav, i denne protokollen, hver testet sensor viste en responstid under femten sekunder. Pastaer som brukes i denne protokollen ble også valgt for sin selektivitet innenfor in vivo miljøer, som inneholder electroactive biomolekyler som kan forstyrre elektrode respons. I tillegg til å lime sammensetning, ble de opererer p...

<table border="1"><tbody><tr><td> Navn på reagensen </td><td> Firma </td><td> Katalognummer </td></tr><tr><td> Flat fleksibel kabel </td><td> Molex </td><td> 3302/10SF </td></tr><tr><td> 0.003 &quot;Side tape </td><td> Melinex </td><td></td></tr><tr><td> 0.004 &quot;Dobbeltsidig tape </td><td> Melinex </td><td></td></tr><tr><td> Laktat oksidase </td><td> Sigma </td><td> L0638 </td></tr><tr><td> Glukose oksidase </td><td> Sigma </td><td> G7141 </td></tr><tr><td> Rhodium på karbon </td><td> Sigma </td><td> 206164 </td></tr><tr><td> Grafitt pulver </td><td> Sigma </td><td> 385031000 </td></tr><tr><td> poly (ethylenimine) </td><td> ACROS </td><td> 178570010 </td></tr><tr><td> Mineralolje </td><td> Sigma </td><td> M5904 </td></tr><tr><td> Glukose </td><td> Sigma &lt;/ Td&gt; <td> G8270 </td></tr><tr><td> Laktat </td><td> Sigma </td><td> L1750 </td></tr><tr><td> Fast Blå RR salt </td><td> Sigma </td><td> F0500 </td></tr><tr><td> e-Shell 300 </td><td> EnvisionTEC </td><td></td></tr><tr><td> e-Shell 200 </td><td> EnvisionTEC </td><td></td></tr><tr><td> Ag / AgCl referanse elektrode </td><td> Basi </td><td> MF-2052 </td></tr><tr><td> Pt ledning </td><td> Basi </td><td></td></tr><tr><td> PGSTAT12 AutolabPotentiostat </td><td> EcoChemie </td><td></td></tr><tr><td> Perfactory RP </td><td> EnvisionTEC </td><td></td></tr><tr><td> Ottoflash Postcuring system </td><td> EnvisionTEC </td><td></td></tr><tr><td> Fosforsyre </td><td> Fisher </td><td> A366-4 </td></tr><tr><td> 60W Modell 6,75 CO 2 raster / vektor laser system </td><td> Universal Laser Systems </td><td> PLS6.75 </td></tr><tr><td> CorelDraw </td><td> Corel </td><td></td></tr><tr><td> SolidWorks </td><td> Dassault Systemes </td><td> 2009 </td></tr><tr><td> Sjarmen RP13 </td><td> Materialise </td><td></td></tr></tbody></table>

hollow microneedle-based sensor for multiplexed transdermal electrochemical sensing

Utviklingen av en minimalt invasiv multiplekset overvåkingssystem for rask analyse av biologisk relevante molekyler kunne tilby personer som lider av kroniske medisinske tilstander lettvinte vurdering av deres umiddelbare fysiologiske tilstand. Videre kan det tjene som et analyseverktøy for analyse av komplekse, multifaktorielle sykdommer. For et slikt multianalyte sensor skal realiseres, må det være minimalt invasiv, prøvetaking av interstitiell væske må skje uten smerte eller skade på brukeren, og analyse må være rask, så vel som selektiv. Opprinnelig utviklet for smerte-fri levering av legemidler, har microneedles blitt brukt til å levere vaksiner og farmakologiske stoffer (f.eks insulin) gjennom huden. 1-2 Siden disse enhetene tilgang til interstitiell plass, kan microneedles som er integrert med microelectrodes brukes som transdermal elektrokjemiske sensorer. Selektiv påvisning av glukose, glutamat, laktat, hydrogen peroxide, og askorbinsyre har blitt demonstrert ved hjelp av integrerte microneedle-elektrode enheter med karbonfibre, modifiserte karbon pastaer, og platina-belagte polymer microneedles tjenestegjør som transducing elementer. 3-7,8 Dette microneedle sensorteknologi har aktivert en roman og sofistikert analytisk tilnærming for in situ og samtidig påvisning av flere analytter. Multiplexing tilbyr muligheten for å overvåke komplekse microenvironments, som ellers er vanskelig å karakterisere på en rask og minimalt invasiv måte. For eksempel kan denne teknologien brukes for samtidig overvåking av ekstracellulære nivåer av, glukose, laktat og pH, 9 som er viktige metabolske indikatorer på sykdom stater 7,10-14 (f.eks kreft spredning) og anstrengelsesutløst acidose. 15

Watch this Scientific Journal Video about Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing at JoVE.com

Hollow Microneedle-based Sensor for Multiplexed Transdermal Electrochemical Sensing

Denne artikkelen detaljer bygging av en multiplekset microneedle-baserte sensor. Enheten er utviklet for in situ prøvetaking og elektrokjemisk analyse av flere analytter i en rask og selektiv måte. Vi ser for klinisk medisin og biomedisinsk forskning bruker for disse microneedle-baserte sensorer.

Hollow microneedle-baserte Sensor for Multiplexed Transdermal Electrochemical Sensing

The development of a minimally invasive multiplexed monitoring system for rapid analysis of biologically-relevant molecules could offer individuals ...

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