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DOI :
10.3791/4083-v
•
14:44 min
September 24th, 2012
Chapters
0:05
Title
2:30
Cell Lysis, Crosslinking and Sample Preparation
5:22
Immunoprecipitation
7:06
Sample Preparation for nano-LC-MS/MS
10:50
Results: HSP70B with its Nucleotide Exchange Factor CGE1
14:07
Conclusion
我们提出了一个快速(的定量免疫共沉淀结合击倒)的方法,前面介绍的区分真假蛋白质 - 蛋白质相互作用的变化。我们的做法是根据 15N代谢标记,ATP,免疫沉淀,并定量质谱的存在/不存在的蛋白质 - 蛋白质相互作用的亲和力的调制。
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