Name: functional assessment of intestinal motility and gut wall inflammation in rodents: analyses in a standardized model of intestinal manipulation
Uploaded: 2012-09-11T12:00:00
Description: Watch this Scientific Journal Video about Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation at JoVE.com

Dette arbejde blev støttet af tilskud fra Deutsche Forschungsgemeinschaft (KA1270/3-1/2) og BONFOR (O-112,0040).

1. Dyr Male C57BL6 / J mus med en gennemsnitlig vægt på 25 g blev opnået fra Harlan Winkelmann (Borchen, Germany). Alle eksperimenter blev udført i overensstemmelse med den føderale lovgivning om beskyttelse af dyr. Principperne for laboratoriedyr pleje blev fulgt. Dyr blev holdt i en 12-timers lys / mørke-cyklus og er forsynet med kommercielt tilgængelige gnaverfoder og ledningsvand ad libitum. Dyr bør anbringes mindst 7 dage efter ankomsten til dit dyr facilitet før eksperi...

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C., Turler, A., Speidel, N., Grandis, J. R., Billiar, T. R., Bauer, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Selective+jejunal+manipulation+causes+postoperative+pan-enteric+inflammation+and+dysmotility.">Selective jejunal manipulation causes postoperative pan-enteric inflammation and dysmotility.</a> Gastroenterology. 26, 159-169 (2004).</li><li>Engel, D. R., Koscielny, A., Wehner, S., Maurer, J., Schiwon, M., Franken, L., Schumak, B., Limmer, A., Sparwasser, T., Hirner, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=T+helper+type+1+memory+cells+disseminate+postoperative+ileus+over+the+entire+intestinal+tract.">T helper type 1 memory cells disseminate postoperative ileus over the entire intestinal tract.</a> Nat. 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En sammenlignende undersøgelse analysere forskellige niveauer af IM (tyndtarmen eventration i 10 minutter versus forsigtigt inspektion af tyndtarmen ved hjælp af to bomuld applikatorer versus IM med evakuering af tyndtarmen indhold i coecum) afslørede en sammenhæng mellem omfanget af kirurgisk manipulation og POI. Sammenlignet med de øvrige grupper IM viste det højeste beløb af leukocytter i ME (PNMs, makrofager og mastceller), hvilket resulterer i forlænget og alvorlig POI. 8. <p class="jove_cont...

<table border="1"><tbody><tr><td> Navn på reagens / udstyr </td><td> Firma </td><td> Katalognummer </td></tr><tr><td> Ethilon 5/0 </td><td> Ethicon </td><td> 1666H </td></tr><tr><td> Bomuld applikatorer </td><td> Lemlæstet </td><td> 71.010 </td></tr><tr><td> arteriekateter </td><td> Vygon </td><td> 115-798 </td></tr><tr><td> Plade med 96 brønde (sort) </td><td> Greiner Bio One </td><td> 655.096 </td></tr><tr><td> glaskugle (2 mm diameter) </td><td> Worf </td><td> rekvireres </td></tr><tr><td> Insect pins </td><td> Fin Science Tools </td><td> 26.000-25 </td></tr><tr><td> Hanker Yates Reagent </td><td> Polyscience </td><td> 560.694 </td></tr><tr><td> Aquatek </td><td> Merck </td><td> HC109850 </td></tr><tr><td> DMEM </td><td> Lonza </td><td> BE12-604 F </td></tr><tr><td> FITC-dextran (MW 70.000) </td><td> Sigma Aldrich </td><td> 46945-500mg-F </td></tr></tbody></table>

functional assessment of intestinal motility and gut wall inflammation in rodents: analyses in a standardized model of intestinal manipulation

Betændelse i mave-tarmkanalen er en almindelig årsag til en række humane sygdomme. Animal forskning modeller er kritisk at undersøge det komplekse cellulære og molekylære af intestinal patologi. Selvom tunica mucosa er ofte orglet af interesse i mange inflammatoriske sygdomme, seneste viste værker, muscularis externa (ME) er også en meget immunokompetente organ, som huser et tæt net af hjemmehørende immunocytter. 1,2 Disse værker blev udført inden for det standardiserede model af intestinal manipulation (IM), der fører til inflammation af tarmvæggen, hovedsageligt begrænset til ME. Klinisk denne inflammation fører til langvarig intestinal dysmotilitet, kendt som postoperativ ileus (POI), som er en hyppig og uundgåelig komplikation efter abdominal kirurgi. 3. inflammation er kendetegnet ved frigivelse af proinflammatoriske mediatorer, såsom IL-6 4 eller IL-1β eller inhibitoriske neurotransmittere ligesom Nitric oxid (NO). 5 Efterfølgende enorme antal immunocytter ekstravasere ind i ME, domineret af polymorfonukleære neutrofiler (PMN) og monocytter og endelig vedligeholde POI. 2 Varig i dagevis, denne intestinal lammelse fører til en øget risiko for aspiration, bakteriel translokation og infektiøse komplikationer op til sepsis og multi organsvigt og forårsager en stor økonomisk belastning. 6 I dette manuskript vi vise den standardiserede model af IM og in vivo vurdering af gastrointestinal transit (GIT) og colon transit. Derudover viser vi en fremgangsmåde til adskillelse af ME fra tunica mucosa efterfulgt af immunologisk analyse, hvilket er afgørende at skelne mellem de inflammatoriske responser i disse både højt immunoaktivt tarmvæggen rum. Alle analyser er let overføres til andre forskningsinstitutioner modeller, der påvirker mave funktion.

Watch this Scientific Journal Video about Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation at JoVE.com

Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation

Postoperativ ileus (POI) er en komplikation af abdominal kirurgi fører til øget sygelighed og en forlænget sygehusophold. Fordi profylaktiske eller terapeutiske strategier mangler intensiveret forskning er nødvendig. Derfor har vi etableret et standardiseret og muligt musemodel til at undersøge patofysiologien af ​​POI og til at studere potentielle terapeutiske muligheder.

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